A comparative proteomic analysis of Desulfovibrio vulgaris Hildenborough in response to the antimicrobial agent free nitrous acid

Gao, Shuhong; Ho, Jun Yuan; Lü, Fan; Nouwens, Amanda; Hoelzle, Robert D.; Schulz, Benjamin L.; Guo, Jianhua; Zhou, Jizhong; Yuan, Zhiguo; Bond, Philip L.

doi:10.1016/j.scitotenv.2019.03.442

Cited by 14 publications

(4 citation statements)

References 49 publications

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“…An exposure time of 6 h to the pharmaceuticals was applied for the proteomic investigations. This enables enough time to detect changes in the levels of proteins expressed in response to an external stress [ 38 ]. Bacterial total protein was extracted after exposure to 5 mg/L of ibuprofen, naproxen, gemfibrozil, diclofenac, propranolol or iopromide.…”

Section: Methodsmentioning

confidence: 99%

Non-antibiotic pharmaceuticals enhance the transmission of exogenous antibiotic resistance genes through bacterial transformation

et al. 2020

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Antibiotic resistance is a serious global threat for public health. Considering the high abundance of cell-free DNA encoding antibiotic resistance genes (ARGs) in both clinical and environmental settings, natural transformation is an important horizontal gene transfer pathway to transmit antibiotic resistance. It is acknowledged that antibiotics are key drivers for disseminating antibiotic resistance, yet the contributions of non-antibiotic pharmaceuticals on transformation of ARGs are overlooked. In this study, we report that some commonly consumed non-antibiotic pharmaceuticals, at clinically and environmentally relevant concentrations, significantly facilitated the spread of antibiotic resistance through the uptake of exogenous ARGs. This included nonsteroidal anti-inflammatories, ibuprofen, naproxen, diclofenac, the lipid-lowering drug, gemfibrozil, and the β-blocker propranolol. Based on the results of flow cytometry, whole-genome RNA sequencing and proteomic analysis, the enhanced transformation of ARGs was affiliated with promoted bacterial competence, enhanced stress levels, over-produced reactive oxygen species and increased cell membrane permeability. In addition, a mathematical model was proposed and calibrated to predict the dynamics of transformation during exposure to non-antibiotic pharmaceuticals. Given the high consumption of non-antibiotic pharmaceuticals, these findings reveal new concerns regarding antibiotic resistance dissemination exacerbated by non-antibiotic pharmaceuticals.

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Section: Methodsmentioning

confidence: 99%

Non-antibiotic pharmaceuticals enhance the transmission of exogenous antibiotic resistance genes through bacterial transformation

et al. 2020

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“…More recently, the HCP was shown to act as highaffinity nitric oxide (NO)-reductase, detoxifying NO into nitrous oxide N 2 O at physiological concentrations, and its overexpression protects cells against nitrosative stress (Kim et al, 2003;Wang et al, 2016;Hagen, 2019). Accordingly, HCP production depends on a NO-inducible promotor in some organisms, in agreement with a possible role in the nitrate/nitrite reduction pathway as the nitrite reduction is a source of NO (Filenko et al, 2007;Spiro, 2007;Wang et al, 2016;Bulot et al, 2019;Gao et al, 2019;Yu et al, 2020). The similar effects/co-occurrence of reactive oxygen species, NO/ nitrosylative stress, and the promiscuity of NO for oxidative stress sensors would explain the production of HCP during oxidative stress (Filenko et al, 2007).…”

Section: Introductionmentioning

confidence: 91%

Structural and biochemical elucidation of class I hybrid cluster protein natively extracted from a marine methanogenic archaeon

Lemaire,

Belhamri,

Wagner

2023

Front. Microbiol.

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Whilst widespread in the microbial world, the hybrid cluster protein (HCP) has been paradoxically a long-time riddle for microbiologists. During three decades, numerous studies on a few model organisms unravelled its structure and dissected its metal-containing catalyst, but the physiological function of the enzyme remained elusive. Recent studies on bacteria point towards a nitric oxide reductase activity involved in resistance during nitrate and nitrite reduction as well as host infection. In this study, we isolated and characterised a naturally highly produced HCP class I from a marine methanogenic archaeon grown on ammonia. The crystal structures of the enzyme in a reduced and partially oxidised state, obtained at a resolution of 1.45 and 1.36-Å, respectively, offered a precise picture of the archaeal enzyme intimacy. There are striking similarities with the well-studied enzymes from Desulfovibrio species regarding sequence, kinetic parameters, structure, catalyst conformations, and internal channelling systems. The close phylogenetic relationship between the enzymes from Methanococcales and many Bacteria corroborates this similarity. Indeed, Methanococcales HCPs are closer to these bacterial homologues than to any other archaeal enzymes. The relatively high constitutive production of HCP in M. thermolithotrophicus, in the absence of a notable nitric oxide source, questions the physiological function of the enzyme in these ancient anaerobes.

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“…Potential QS mechanisms as FNA is able to passively pass through bacterial cytoplasmic membranes and interact with enzymes that contain thiols, heme groups, iron sulfur clusters, phenolic or aromatic amino acid residues, tyrosyl radicals, and amines ( Zhou et al, 2008 ; Gao et al, 2019 ), the activity of AHL synthase can be easily inhibited, thus influencing the synthesis of AHL. Similar effects might occur on LuxR and receptor protein of AHL, therefore, hinder the regulation of QS on nitrifying bacteria community.…”

Section: Function Of Fna On Pn Establishmentmentioning

confidence: 99%

Achieving Partial Nitritation by Treating Sludge With Free Nitrous Acid: The Potential Role of Quorum Sensing

Jiang

Wang

et al. 2022

Front. Microbiol.

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Partial nitritation is increasingly regarded as a promising biological nitrogen removal process owing to lower energy consumption and better nitrogen removal performance compared to the traditional nitrification process, especially for the treatment of low carbon wastewater. Regulating microbial community structure and function in sewage treatment systems, which are mainly determined by quorum sensing (QS), by free nitrous acid (FNA) to establish a partial nitritation process is an efficient and stable method. Plenty of research papers reported that QS systems ubiquitously existed in ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB), and various novel nitrogen removal processes based on partial nitritation were successfully established using FNA. Although the probability that partial nitritation process might be achieved by the regulation of FNA on microbial community structure and function through the QS system was widely recognized and discussed, the potential role of QS in partial nitritation achievement by FNA and the regulation mechanism of FNA on QS system have not been reviewed. This article systematically reviewed the potential role of QS in the establishment of partial nitritation using FNA to regulate activated sludge flora based on the summary and analysis of the published literature for the first time, and future research directions were also proposed.

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A comparative proteomic analysis of Desulfovibrio vulgaris Hildenborough in response to the antimicrobial agent free nitrous acid

Cited by 14 publications

References 49 publications

Non-antibiotic pharmaceuticals enhance the transmission of exogenous antibiotic resistance genes through bacterial transformation

Non-antibiotic pharmaceuticals enhance the transmission of exogenous antibiotic resistance genes through bacterial transformation

Structural and biochemical elucidation of class I hybrid cluster protein natively extracted from a marine methanogenic archaeon

Achieving Partial Nitritation by Treating Sludge With Free Nitrous Acid: The Potential Role of Quorum Sensing

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