A comparative proteomic analysis of Gluconacetobacter diazotrophicus PAL5 at exponential and stationary phases of cultures in the presence of high and low levels of inorganic nitrogen compound

Lery, Letícia Miranda Santos; Krüger, Wanda M. A. von; Viana, Fernanda Carvalho; Teixeira, K. R. dos S.; Bisch, Paulo Mascarello

doi:10.1016/j.bbapap.2008.06.020

Cited by 15 publications

(10 citation statements)

References 120 publications

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“…The reason for differential regulation of this protein upon exposure to plant macerate is currently unknown. Interestingly, RpsA expression has also been shown to be regulated in other plant-bacteria associations; for instance, in the sugar cane associated bacterium Gluconacetobacter diazotrophicus (dos Santos et al 2010;Lery et al 2008) Elongation factor (EF)-Tu is one of the most abundant proteins in bacteria and it plays an essential role in the elongation phase of protein biosynthesis. However EF-Tu, just like flagellin, is also recognized by the plant as a PAMP, activating the plant innate immune response (Kunze et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Plant-Influenced Gene Expression in the Rice Endophyte Burkholderia kururiensis M130

Coutinho¹,

Licastro²,

Mendonça-Previato³

et al. 2015

MPMI

111

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Burkholderia kururiensis M130 is one of the few rice endophytic diazotrophic bacteria identified thus far which is able to enhance growth of rice. To date, very little is known of how strain M130 and other endophytes enter and colonize plants. Here, we identified genes of strain M130 that are differentially regulated in the presence of rice plant extract. A genetic screening of a promoter probe transposon mutant genome bank and RNAseq analysis were performed. The screening of 10,100 insertions of the genomic transposon reporter library resulted in the isolation of 61 insertions displaying differential expression in response to rice macerate. The RNAseq results validated this screen and indicated that this endophytic bacterium undergoes major changes in the presence of plant extract regulating 27.7% of its open reading frames. A large number of differentially expressed genes encode membrane transporters and secretion systems, indicating that the exchange of molecules is an important aspect of bacterial endophytic growth. Genes related to motility, chemotaxis, and adhesion were also overrepresented, further suggesting plant–bacteria interaction. This work highlights the potential close signaling taking place between plants and bacteria and helps us to begin to understand the adaptation of an endophyte in planta.

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Section: Discussionmentioning

confidence: 99%

Plant-Influenced Gene Expression in the Rice Endophyte Burkholderia kururiensis M130

Coutinho¹,

Licastro²,

Mendonça-Previato³

et al. 2015

MPMI

111

View full text Add to dashboard Cite

show abstract

“…The genome size is in the average of others AAB genome already published . Further studies of this microorganism functional genomics have been underway by several researchers group and are leading to a comprehension of the role of its gene contents to its general metabolism, nitrogen fixation, and others plant growth mechanisms . In addition, several mutants have already been obtained and used for functional genomic characterization .…”

Section: The Gluconacetobacter Genusmentioning

confidence: 99%

Nitrogen fixing bacteria in the family Acetobacteraceae and their role in agriculture

Reis

Teixeira

2015

J. Basic Microbiol.

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For centuries, the Acetobacteraceae is known as a family that harbors many species of organisms of biotechnological importance for industry. Nonetheless, since 1988 representatives of this family have also been described as nitrogen fixing bacteria able to plant growth promotion by a variety of mechanisms. Nitrogen fixation is a biological process that guarantees that the atmospheric N2 is incorporated into organic matter by several bacterial groups. Most representatives of this group, also known as diazotrophic, are generally associated with soil rhizosphere of many plants and also establishing a more specific association living inside roots, leaves, and others plants tissues as endophyte. Their roles as plant growth-promoting microorganisms are generally related to increase in plant biomass, phosphate and other mineral solubilization, and plant pathogen control. Here, we report many of these plant growth-promoting processes related to nitrogen fixing species already described in Acetobacteraceae family, especially Gluconacetobacter diazotrophicus and their importance to agriculture. In addition, a brief review of the state of art of the phylogenetics, main physiological and biochemical characteristics, molecular and functional genomic data of this group of Acetobacteraceae is presented.

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“…Protein identification was performed by liquid chromatography tandem mass spectrometry (LC-MS/MS) using a Q-TOF Ultima API mass spectrometer (Micromass, Manchester, UK) to analyze tryptic peptides according to Lery et al (2008). A nanoflow ESI source was used with a lock spray source for lock mass measurements during all the chromatographic runs.…”

Section: Secretome Analysesmentioning

confidence: 99%

Genomic and proteomic analyses of the coral pathogen Vibrio coralliilyticus reveal a diverse virulence repertoire

et al. 2011

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Vibrio coralliilyticus has been implicated as an important pathogen of coral species worldwide. In this study, the nearly complete genome of Vibrio coralliilyticus strain P1 (LMG23696) was sequenced and proteases implicated in virulence of the strain were specifically investigated. The genome sequence of P1 (5 513 256 bp in size) consisted of 5222 coding sequences and 58 RNA genes (53 tRNAs and at least 5 rRNAs). Seventeen metalloprotease and effector (vgrG, hlyA and hcp) genes were identified in the genome and expressed proteases were also detected in the secretome of P1. As the VcpA zinc-metalloprotease has been considered an important virulence factor of V. coralliilyticus, a vcpA deletion mutant was constructed to evaluate the effect of this gene in animal pathogenesis. Both wild-type and mutant (DvcpA) strains exhibited similar virulence characteristics that resulted in high mortality in Artemia and Drosophila pathogenicity bioassays and strong photosystem II inactivation of the coral dinoflagellate endosymbiont (Symbiodinium). In contrast, the DvcpA mutant demonstrated higher hemolytic activity and secreted 18 proteins not secreted by the wild type. These proteins included four types of metalloproteases, a chitinase, a hemolysin-related protein RbmC, the Hcp protein and 12 hypothetical proteins. Overall, the results of this study indicate that V. coralliilyticus strain P1 has a diverse virulence repertoire that possibly enables this bacterium to be an efficient animal pathogen.

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A comparative proteomic analysis of Gluconacetobacter diazotrophicus PAL5 at exponential and stationary phases of cultures in the presence of high and low levels of inorganic nitrogen compound

Cited by 15 publications

References 120 publications

Plant-Influenced Gene Expression in the Rice Endophyte Burkholderia kururiensis M130

Plant-Influenced Gene Expression in the Rice Endophyte Burkholderia kururiensis M130

Nitrogen fixing bacteria in the family Acetobacteraceae and their role in agriculture

Genomic and proteomic analyses of the coral pathogen Vibrio coralliilyticus reveal a diverse virulence repertoire

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