A comparative pharmacokinetic study of three flavonoids and three anthraquinones in normal and gastrointestinal motility disorders rat plasma after the oral administration of Wei‐Chang‐Shu tablet using high‐performance liquid chromatography–tandem mass spectrometry

Abstract

A simple, fast and reliable high-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification and pharmacokinetic study of three flavonoids (liquiritigenin, isoliquiritigenin and formononetin) and three anthraquinones (emodin, rhein and aloe-emodin), which are the bioactive ingredients of Wei-Chang-Shu tablet found in rat plasma. After extraction by liquid-liquid extraction with ethyl acetate, chromatographic separation was achieved on an Agilent Zorbax SB-C column (4.6 × 150 mm, 5 μm) at a flow rate of 1 mL/min by gradient elution using 0.1% aqueous acetic acid and acetonitrile. The detection was performed using a triple quadrupole mass spectrometer equipped with electrospray ionization source in the negative ionization and selected reaction monitoring mode. Method validation was performed in terms of specificity, carryover, linearity (r > 0.99), intra-/inter-day precision (1.0-10.1%), accuracy (relative error, <7.6%), stability (0.6-13.2%), extract recovery (74.9-91.9%) and matrix effect (89.1-109%). The lower limits of quantification of the six analytes varied from 0.92 to 10.4 ng/mL. The validated method was successfully applied to compare the pharmacokinetic properties of Wei-Chang-Shu tablet in normal rats and in rats with gastrointestinal motility disorders. The results indicated that there were obvious differences in the pharmacokinetic behavior between normal and model rats. This study will be helpful in the clinical application of Wei-Chang-Shu tablet.

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“…Emodin has liver protective properties ( 19 ), anti-inflammatory ( 20 , 21 ), and anti-viral effects ( 22 ). It is also associated with immune regulation ( 23 ), promotion of gastrointestinal motility ( 24 ), as well as antioxidant properties ( 25 ).…”

Integrated pharmacokinetics of Huangqi Jianzhong Tang in normal and chronic atrophic gastritis rats

“…This research revealed that the plasma contents of 12 prototypical components and one metabolite in the plasma of HQJZ were decreased under the disease condition, while the retention time of flavonoids and flavonoid glycosides became longer in vivo . The pharmacokinetic comparison of three flavonoids (liquiritigenin, isoquiritigenin and formononetin) in WeiChangShu Tablets in normal and gastrointestinal peristaltic rats was consistent with our results, where the AUC (0– t ) , AUC (0–∞) and C max decreased and the T 1/2 increased significantly (Ren et al, 2017). Fang et al found that the AUC (0– t ) and C max decreased and the T 1/2 and MRT (0– t ) increased significantly by comparing the pharmacokinetic parameters of puerarin in healthy and functional dyspepsia patients (Qin et al, 2009).…”

“…Traditional Chinese medicine holds the characteristics of being multicomponent and multitarget, and so a PK study of single components cannot reflect its integrity. Wang Guangji et al proposed the T A B L E 1 Comparison of pharmacokinetic parameters of Huangqi Jianzhong Tang (HQJZ) in control and model rats testinal peristaltic rats was consistent with our results, where the AUC (0-t) , AUC (0-∞) and C max decreased and the T 1/2 increased significantly (Ren et al, 2017). Fang et al found that the AUC (0-t) and C max decreased and the T 1/2 and MRT (0-t) increased significantly by comparing the pharmacokinetic parameters of puerarin in healthy and functional dyspepsia patients (Qin et al, 2009).…”

Metabolomics Analysis of L-Arginine Induced Gastrointestinal Motility Disorder in Rats Using UPLC-MS After Magnolol Treatment

“…The pathogenic factors of gastrointestinal motility disorder are complex. At present, there is no objective criterion for gastrointestinal motility disorder model, but atropine and L-arginine are commonly selected drugs for this model (Ren et al, 2017; Wang et al, 2018). The results of gastric emptying and intestinal propulsion also indicate that L-arginine had inhibited gastric emptying and intestinal propulsion, which indicated that the model of gastrointestinal motility disorder was successful.…”