A Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter pylori Isolates from Southern Russia

Сорокин, В. М.; Pisanov, R. V.; Голубкина, Е. В.; Березняк, Елена Александровна; Prozorova, Lubov Aleksandrovna

doi:10.9734/bpi/rpmb/v7/3230f

Cited by 2 publications

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“…MLVA technique used in the current study determined a set of similarities in some A. baumannii strains isolated from animal meat and human clinical samples. This technique has been used for molecular typing of different pathogenic agents, especially Salmonella enterica [34], Escherichia coli [35], Staphylococcus aureus [36], Helicobacter pylori [37] and Listeria monocytogenes [38]. Hauck et al [39] reported the MLVA-based molecular typing of A. baumannii strains isolated from French hospital clinical samples.…”

Section: Discussionmentioning

confidence: 99%

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Multiple locus variable number tandem repeat analysis of Acinetobacter baumannii strains isolated from human clinical and animal meat samples

Karimi

Momtaz

Fazel

2021

Reviews in Medical Microbiology

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The current research was done to study the prevalence rate and molecular typing of Acinetobacter baumannii strains isolated from human and animal samples. Onehundred and seventy-four animal meat and 128 human clinical samples were collected and subjected to bacterial culture. A. baumannii isolates were confirmed using the Loop-mediated isothermal amplification. Approved strains were subjected to molecular typing using the multiple-locus variable number tandem repeat analysis method. Fortyfour out of 174 (25.28%) raw meat and 64 out of 128 (50%) human samples were positive for A. baumannii strains. Ovine meat (39.28%) and urine (56.06%) samples had the highest prevalence of A. baumannii strains. Eighteen human isolates were located in eight separate profiles, whereas 18 animal isolates were located in six separate profiles. The highest similarities were found between human-based A. baumannii isolates nos 6, 7 and 18 with isolates nos 5, 11, 13 and 15 (85.6% similarity). The highest similarities were found between animal-based A. baumannii isolates nos 10, 11 and 17 (99.8% similarity). From a total of 10 studied variable copy numbers of tandem repeats (VNTR) loci, 0845, 0826 and 3406 were detected in all animal-based A. baumannii isolates. Moreover, 3406 VNTR loci was only detected in all 18 human-based A. baumannii isolates. A. baumannii isolate no 17 (harbored all 10 VNTR loci) and A. baumannii isolates nos 6, 7 and 18 (harbored 9 VNTR loci) were the most pathogenic human and animal-based strains. Multiple-locus variable number tandem repeat analysis was considered as an accurate and practical method for molecular typing of A. baumannii strains.

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Section: Discussionmentioning

confidence: 99%

“…Staphylococcus aureus [36], Helicobacter pylori [37] and Listeria monocytogenes [38]. Hauck et al [39] reported the MLVA-based molecular typing of A. baumannii strains isolated from French hospital clinical samples.…”

Section: Discussionmentioning

confidence: 99%

Multiple locus variable number tandem repeat analysis of Acinetobacter baumannii strains isolated from human clinical and animal meat samples

Karimi

Momtaz

Fazel

2021

Reviews in Medical Microbiology

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“…Следует упомянуть, что начиная с Маастрихт 5 появились рекомендации о 14-дневном курсе антихеликобактерной терапии, и эти сроки сохраняются для основных схем эрадикации первой линии в рекомендациях Маастрихт 6 [18]). Совершенно [19].…”

Section: глава 2 отдалённые результаты антихеликобактерной терапии -э...unclassified

<i>Helicobacter pylori</i> Persistence in Patients with Peptic Ulcer Disease and Chronic Gastritis in the Treatment with Antibiotics and Acid-Suppressive Agents

Golubkina

2023

A&Ch

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Background. Chronic gastritides still have no division into forms according to the data of the genotyping of Helicobacter pylori isolates. Monitoring the results of anti-Helicobacter pylori therapy cannot be limited to eradication, by taking into account the data on Helicobacter pylori detection in healthy individuals. Is increased acid production associated with the presence of virulent or nonvirulent strains?Objective. To search for the predominance of cagA or any allelic combination of vacA in the isolates from patients with mild and severe chronic gastritis and from those with peptic ulcer disease. To track the time course of changes in the disappearance and repeated gastric colonization with different Helicobacter pylori strains in patients with peptic ulcer disease immediately after anti-Helicobacter pylori therapy and 1.5–2 months later. To confirm or refute the fact that the increased acid production is associated with the presence of virulent Helicobacter pylori strains rather than with the persistence of nonvirulent strains.Methods. The Helicobacter pylori isolates from the gastric biopsy specimens taken from patients with chronic gastritis and peptic ulcer disease were genotyped for cagA and allelic combinations of vacA. The strains from patients who had received anti-Helicobacter pylori therapy were genotyped using the variable number tandem repeat (VNTR) method. Patients with an exacerbation of peptic ulcer disease and those wth an exacerbation of chronic pancreatitis with concomitant gastritis were compared from the level of acid production and from the belonging of Helicobacter pylori to virulent strains.Results. Patients with mild chronic gastritis were observed to have an abundance of nonvirulent allelic combination of vacAs2m2, which differed significantly from its frequency in both the patients with severe chronic gastritis and those with peptic ulcer disease. In the latter patients, the differences between the background bacteriological pattern and the pattern observed after 1.5–2 months had a high degree of significance; in these follow-up periods, in contrast to the control immediately after therapy, the number of eradications increased and the number of cagA-containing strains decreased due to the change of strains. Acid production was significantly higher in the patients with peptic ulcer disease than in those with chronic pancreatits and concomitant gastritis; the former patients show a statistically significant predominance of virulent strains; the latter patients had nonvirulent strains (at the same rate of Helicobacter pylori detection).Conclusion. Filling the blank spots in the study of helicobacteriosis remains relevant due to the progress of genetic methods in bacteriology and to the introduction of novel approaches to assessing the results of anti-Helicobacter pylori therapy.

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A Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter pylori Isolates from Southern Russia

Cited by 2 publications

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Multiple locus variable number tandem repeat analysis of Acinetobacter baumannii strains isolated from human clinical and animal meat samples

Multiple locus variable number tandem repeat analysis of Acinetobacter baumannii strains isolated from human clinical and animal meat samples

<i>Helicobacter pylori</i> Persistence in Patients with Peptic Ulcer Disease and Chronic Gastritis in the Treatment with Antibiotics and Acid-Suppressive Agents

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