A comparative morphological, electrophysiological and functional analysis of axon regeneration through peripheral nerve autografts genetically modified to overexpress BDNF, CNTF, GDNF, NGF, NT3 or VEGF

Hoyng, Stefan A.; Winter, Fred de; Gnavi, Sara; Boer, Ralph de; Boon, Lennard I.; Korvers, Laura M.; Tannemaat, Martijn R.; Malessy, Martijn J. A.; Verhaagen, Joost

doi:10.1016/j.expneurol.2014.08.002

Cited by 87 publications

(97 citation statements)

References 76 publications

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“…Increase in the expression of GNDF from the GNDF family and IL-6 from the neuropoeitic cytokine and decrease in the expression of CNTF (ciliary neurotrophic factor) have also been reported. While NGF mRNA can hardly be detected in intact nerve normally, it has been reported to increase 10 times in the distal part within the first 12 hours following damage and to decrease to 5 times of the normal level on the 72nd hour and stay at this level for about three weeks (25,(33)(34)(35). The BDNF mRNA increase, which occurs in the distal part following the damage, is quite slow when compared with NGF.…”

Section: Interactıons Between Neurotrophıc Factorsmentioning

confidence: 96%

“…However, the increase in BDNF mRNA expression reaches a detectable level on the 7th day following the damage and continues to increase until the 28th day. The maximum level of BDNF mRNA is about ten times more than that of NGF mRNA (25,35,36). The expression of NT-3, one of the members of neurotrophin family, can be easily detected in healthy nerves with NT-3 mRNA and its expression within 12 hours following the damage has been reported to decrease rapidly and return to basal level within 2 weeks.…”

Section: Interactıons Between Neurotrophıc Factorsmentioning

confidence: 99%

“…One of the most important factors of poor recovery in motor functions following peripheral nerve injury is the decrease in the axonal regeneration abilities of motor neurons. During regeneration, a time-dependent decrease is seen in the total number of regenerated axons and reinnervation of muscle fibers (25,47). However, it is suggested that low dose and long term BDNF application increases both axonal regeneration and neural cell repair and thus it is very effective in reversing the negative effects caused by chronic axotomy (38).…”

Section: Neurotrophinsmentioning

confidence: 99%

“…CNTF and neurotrophins are known to play an important role separately or in combination in axonal outgrowth and functional survival after damage (25). Studies have reported that the combinations of CNTF / BDNF treatment has neuroprotective role in the retina (60).…”

Section: Neuropoietic Cytokinesmentioning

confidence: 99%

See 3 more Smart Citations

The role of growth factors in nerve regeneration

Önger

Delibaş

Türkmen

et al. 2016

DD&T

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Section: Interactıons Between Neurotrophıc Factorsmentioning

confidence: 96%

Section: Interactıons Between Neurotrophıc Factorsmentioning

confidence: 99%

Section: Neurotrophinsmentioning

confidence: 99%

Section: Neuropoietic Cytokinesmentioning

confidence: 99%

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The role of growth factors in nerve regeneration

Önger

Delibaş

Türkmen

et al. 2016

DD&T

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“…42,43 In the present study, magnetic membranes via MFs were capable of increasing the expression and secretion of BDNF, GDNF, NT-3, and VEGF in SCs, which was further enhanced when SCs were seeded within scaffolds, and moreover, the gene expression and protein secretion of these neurotrophic factors through a time-course assay were reversible, which has shown that high prolonged expression of these factors is not necessarily positive in peripheral nerve repair. 44 All these findings indicated that magnetic nanocomposites via MFs are capable of regulating the expression and secretion of multiple NTs in a synergistic way in SCs.…”

mentioning

confidence: 92%

Activation of Schwann cells in vitro by magnetic nanocomposites via applied magnetic field

Liu¹,

Huang²,

Liu³

et al. 2014

IJN

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Schwann cells (SCs) are attractive seed cells in neural tissue engineering, but their application is limited by attenuated biological activities and impaired functions with aging. Therefore, it is important to explore an approach to enhance the viability and biological properties of SCs. In the present study, a magnetic composite made of magnetically responsive magnetic nanoparticles (MNPs) and a biodegradable chitosan–glycerophosphate polymer were prepared and characterized. It was further explored whether such magnetic nanocomposites via applied magnetic fields would regulate SC biological activities. The magnetization of the magnetic nanocomposite was measured by a vibrating sample magnetometer. The compositional characterization of the magnetic nanocomposite was examined by Fourier-transform infrared and X-ray diffraction. The tolerance of SCs to the magnetic fields was tested by flow-cytometry assay. The proliferation of cells was examined by a 5-ethynyl-2-deoxyuridine-labeling assay, a PrestoBlue assay, and a Live/Dead assay. Messenger ribonucleic acid of BDNF, GDNF, NT-3, and VEGF in SCs was assayed by quantitative real-time polymerase chain reaction. The amount of BDNF, GDNF, NT-3, and VEGF secreted from SCs was determined by enzyme-linked immunosorbent assay. It was found that magnetic nanocomposites containing 10% MNPs showed a cross-section diameter of 32.33±1.81 µm, porosity of 80.41%±0.72%, and magnetization of 5.691 emu/g at 8 kOe. The 10% MNP magnetic nanocomposites were able to support cell adhesion and spreading and further promote proliferation of SCs under magnetic field exposure. Interestingly, a magnetic field applied through the 10% MNP magnetic scaffold significantly increased the gene expression and protein secretion of BDNF, GDNF, NT-3, and VEGF. This work is the first stage in our understanding of how to precisely regulate the viability and biological properties of SCs in tissue-engineering grafts, which combined with additional molecular factors may lead to the development of new nerve grafts.

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3D Printed Personalized Nerve Guide Conduits for Precision Repair of Peripheral Nerve Defects

Yan

et al. 2022

Advanced Science

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The treatment of peripheral nerve defects has always been one of the most challenging clinical practices in neurosurgery. Currently, nerve autograft is the preferred treatment modality for peripheral nerve defects, while the therapy is constantly plagued by the limited donor, loss of donor function, formation of neuroma, nerve distortion or dislocation, and nerve diameter mismatch. To address these clinical issues, the emerged nerve guide conduits (NGCs) are expected to offer effective platforms to repair peripheral nerve defects, especially those with large or complex topological structures. Up to now, numerous technologies are developed for preparing diverse NGCs, such as solvent casting, gas foaming, phase separation, freeze‐drying, melt molding, electrospinning, and three‐dimensional (3D) printing. 3D printing shows great potential and advantages because it can quickly and accurately manufacture the required NGCs from various natural and synthetic materials. This review introduces the application of personalized 3D printed NGCs for the precision repair of peripheral nerve defects and predicts their future directions.

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A comparative morphological, electrophysiological and functional analysis of axon regeneration through peripheral nerve autografts genetically modified to overexpress BDNF, CNTF, GDNF, NGF, NT3 or VEGF

Cited by 87 publications

References 76 publications

The role of growth factors in nerve regeneration

The role of growth factors in nerve regeneration

Activation of Schwann cells in vitro by magnetic nanocomposites via applied magnetic field

3D Printed Personalized Nerve Guide Conduits for Precision Repair of Peripheral Nerve Defects

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