A comparative investigation of biochemical and histopathological effects of thiamine and thiamine pyrophosphate on ischemia–reperfusion induced oxidative damage in rat ovarian tissue

Abstract

In this study, the biochemical and histopathological effects of thiamine and thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat ovarian tissue were investigated. Animals were divided into four groups of six rat each, ovarian ischemia-reperfusion (IR), 25 mg/kg thiamine + ovarian ischemia-reperfusion (TIR), 25 mg/kg thiamine pyrophosphate + ovarian ischemia-reperfusion (TPIR) and Sham group (SG). The results of the biochemical experiments have shown that the rat ovarian tissue with thiamine treatment, the level of MDA, GSH and the 8-hydroxyguanine are almost the same as the IR group; while in the group with thiamine pyrophosphate treatment, the level of MDA, GSH and the 8-hydroxyguanine are almost the same as the SG. Ovarian tissue of rats in the IR group were congested and dilated vessels, edema, hemorrhage, necrotic and apoptotic cells. In this group, the migration and the adhesion of the polymorphonuclear leucocytes to the endothelium were observed. Both ovaries in TPIR group, there was no difference according to the SG. Histopathology of ovarian tissues in the TIR group was almost the same with the IR group. Our results indicate that thiamine pyrophosphate significantly prevents the ischemia-reperfusion induced oxidative damage in ovarian tissue, whereas thiamine has no effect. In conclusion, we have found that thiamine pyrophosphate prevents oxidative damage due to ischemia-reperfusion injury, whereas thiamine does not have this effect. Furthermore, we have confirmed that the results of our biochemical analyses are in concordance with the histopathological findings.

Effect of Kineret® on ovarian ischemia reperfusion injury in a rat model

“…3,23 Demiryilmaz et al reported a significantly increased MDA level in rat ovarian tissue subjected to IR. 24 In the present study, a significant increase was also noted in MPO activity in the ovarian tissue of the IRG group, in addition to an increase in MDA levels. Similiarly, previous studies have reported high MPO activity and MDA levels in the ovarian tissues of rat groups subjected to IR.…”

Effect of thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat kidney

“…In a study, it was found that IR induced oxidative damage in rat ovary was not prevented by thiamine, but it was prevented by thiamine pyrophosphate. [8] In addition, unlike thiamine, thiamine pyrophosphate is a cofactor for both pyruvate-2-oxoglutarate dehydrogenase complex that is required for mitochondrial adenosine triphosphate (ATP) synthesis and transketolase enzyme plays an important role in maintaining cellular redox status. [9] Therefore, thiamine pyrophosphate is important for cellular energy production and preservation.…”

Biochemical Investigation of the Protective Effect of Thiamine Pyrophosphate on Methotrexate-Induced Gastrotoxicity in Rats

“…6 Thiamine, although known to have antioxidant properties, 7 couldn't prevent the oxidative damage generated by the ischemia-reperfusion injury in rat ovaries, however thiamine pyrophosphate was found to prevent it. 8 At a scan of literature any research for the prevention of gastric damage due to methotrexate by thiamine pyrophosphate couldn't be found. Therefore, the aim of our study is to investigate the effect of thiamine pyrophosphate on the oxidative damage made by methotrexate in rat stomach biochemically and compare it with thiamine.…”