A comparative GC–MS analysis of bioactive secondary metabolites produced by halotolerant Bacillus spp. isolated from the Great Sebkha of Oran

Nas, Fatima; Aissaoui, Nadia; Mahjoubi, Mouna; Mosbah, Ahmed; Arab, Mounia; Abdelwahed, Soukaina; Khrouf, Rim; Masmoudi, Ahmed-Salaheddine; Chérif, Ameur; Klouche-Khelil, Nihel

doi:10.1007/s10123-021-00185-x

Cited by 19 publications

(11 citation statements)

References 61 publications

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“…According to a recent study, probiotics, particularly lactic acid bacteria (LAB), have drawn much interest from the food and pharmaceutical industries because of their many advantages for human health and well-being. − Several studies have shown that LAB provides biological protection against microbial infections. , As a result, it would be one of the novel approaches to using LAB as an environmentally friendly method of synthesizing biocompatible NPs that can be used to treat a wide variety of microbial infections. , Thus, in the current study, we sought to isolate and characterize the LAB strains from the Kimchi sample as a biological source for the synthesis of AuNPs and AgNPs. In addition, gas chromatography-mass spectroscopy was used to investigate the identified LAB strain C1 for the production of secondary metabolites to determine their various biological functions …”

Section: Introductionmentioning

confidence: 99%

Inhibition of Biofilm and Virulence Properties of Pathogenic Bacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus sp. Strain C1

et al. 2023

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The emergence of antibiotic resistance in microbial pathogens necessitates the development of alternative ways to combat the infections that arise. The current study used nanotechnology as an alternate technique to control virulence characteristics and biofilm development in Pseudomonas aeruginosa and Staphylococcus aureus. Furthermore, based on the acceptance and biocompatibility of the probiotic bacteria, we chose a lactic acid bacteria (LAB) for synthesizing two types of metallic nanoparticles (NPs) in this study. Using molecular techniques, the LAB strain C1 was isolated from Kimchi food samples and identified as Lactiplantibacillus sp. strain C1. The prepared supernatant from strain C1 was used to produce gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs). C1-AuNPs and C1-AgNPs were characterized physiochemically using a variety of instruments. C1-AuNPs and C1-AgNPs had spherical shapes and sizes of 100.54 ± 14.07 nm (AuNPs) and 129.51 ± 12.31 nm (AgNPs), respectively. C1-AuNPs and C1-AgNPs were discovered to have high zeta potentials of −23.29 ± 1.17 and −30.57 ± 0.29 mV, respectively. These nanoparticles have antibacterial properties against several bacterial pathogens. C1-AuNPs and C1-AgNPs significantly inhibited the initial stage biofilm formation and effectively eradicated established mature biofilms of P. aeruginosa and S. aureus. Furthermore, when P. aeruginosa was treated with sub-MIC levels of C1-AuNPs and C1-AgNPs, their different virulence features were significantly reduced. Both NPs greatly inhibited the hemolytic activity of S. aureus. The inhibition of P. aeruginosa and S. aureus biofilms and virulence features by C1-AuNPs and C1-AgNPs can be regarded as viable therapeutic strategies for preventing infections caused by these bacteria.

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Section: Introductionmentioning

confidence: 99%

Inhibition of Biofilm and Virulence Properties of Pathogenic Bacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus sp. Strain C1

et al. 2023

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“…It has been originally isolated from marine Streptomyces sp. VITVSK1 and was also detected in low amounts in Bacillus spp (0.512%; [ 99 ]) as well as orchids B. echinolabium (2.65%; [ 36 ]) and Maxillariella vulcanica (F. Lehm. & Kraenzl.)…”

Section: Discussionmentioning

confidence: 99%

Floral attractants in the black orchid Brasiliorchis schunkeana (Orchidaceae, Maxillariinae): clues for presumed sapromyophily and potential antimicrobial activity

et al. 2022

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Background Orchids have evolved various strategies that aim to ensure their reproduction success. These may include the production of rewards for pollinators, or on the contrary, deception. Specific sets of features such as flower morphology, color, nectar, and odor presence (or lack thereof) are considered to determine suitability for pollination by different groups of animals. Stingless bees are thought to be the primary pollinators of the orchids of the Neotropical subtribe Maxillariinae. However, almost black flowered Brasiliorchis schunkeana at first glance presents floral adaptations that may suggest another pollination syndrome—sapromyophily. Results A few traces of secretion were noticed on the glabrous lip callus and lip apex built by conical to villiform papillae (SEM analysis). Histochemical studies revealed huge amounts of lipids in the epidermis, subepidermis, and some parenchyma cells (SBB test) with various stages of lipids accumulation between cells. Further TEM analysis showed a heterogeneous (lipoid and phenolic) nature of secretion. The dense osmiophilic cytoplasm contained organelles (RER, free ribosomes, dictyosomes, plastids with plastoglobuli, nucleus) and vesicles migrating to plasmalemma. The vesicles, osmiophilic globules, and flocculent material were visible in periplasmic space. The central vacuole possessed osmiophilic phenolic content and flocculent material. GC–MS analysis revealed in floral extract the presence of 7,9-di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione (77.06%) and 2,5-di-tert-butyl-1,4-benzoquinone (16.65%). Both compounds are known for their biological activity. Conclusions The juxtaposition of results led us to the conclusion that the labellar tissue produces lipoid and phenolic material, which is responsible for the glossiness and rotten herring scent. This type of secretion could be classified as a phenolic resin. The chemical analysis revealed the presence of five semiochemicals that are known to be attractants for some Diptera, which together with the rest of the results constitutes a strong premise that representatives of this order could be potential pollinators of B. schunkeana. Field observations however are still needed to confirm this pollination syndrome.

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“…One approach is to discover novel sources for antimicrobial compounds that can be developed into future treatments. In recent years, more and more studies have reported volatiles with antimicrobial activity which indicates that volatiles might play an important role in countering the antimicrobial resistance crisis [ 18 , 19 , 20 , 21 ]. For example, the new volatile antibiotics albaflavenone and pentalenolactone produced by Streptomyces coelicolor and Streptomyces avermitilis , respectively were discovered [ 22 ].…”

Section: Antimicrobial Resistance: a Global Crisismentioning

confidence: 99%

Air Ambulance: Antimicrobial Power of Bacterial Volatiles

2022

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We are currently facing an antimicrobial resistance crisis, which means that a lot of bacterial pathogens have developed resistance to common antibiotics. Hence, novel and innovative solutions are urgently needed to combat resistant human pathogens. A new source of antimicrobial compounds could be bacterial volatiles. Volatiles are ubiquitous produced, chemically divers and playing essential roles in intra- and interspecies interactions like communication and antimicrobial defense. In the last years, an increasing number of studies showed bioactivities of bacterial volatiles, including antibacterial, antifungal and anti-oomycete activities, indicating bacterial volatiles as an exciting source for novel antimicrobial compounds. In this review we introduce the chemical diversity of bacterial volatiles, their antimicrobial activities and methods for testing this activity. Concluding, we discuss the possibility of using antimicrobial volatiles to antagonize the antimicrobial resistance crisis.

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A comparative GC–MS analysis of bioactive secondary metabolites produced by halotolerant Bacillus spp. isolated from the Great Sebkha of Oran

Cited by 19 publications

References 61 publications

Inhibition of Biofilm and Virulence Properties of Pathogenic Bacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus sp. Strain C1

Inhibition of Biofilm and Virulence Properties of Pathogenic Bacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus sp. Strain C1

Floral attractants in the black orchid Brasiliorchis schunkeana (Orchidaceae, Maxillariinae): clues for presumed sapromyophily and potential antimicrobial activity

Air Ambulance: Antimicrobial Power of Bacterial Volatiles

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