A Comparative Evaluation of the Antibacterial Efficacy of Honey In Vitro and Antiplaque Efficacy in a 4‐Day Plaque Regrowth Model In Vivo: Preliminary Results

Aparna, S; Sridharan, Srirangarajan; Malgi, Veena; Setlur, Krishnanand P.; Shashidhar, Ravindranath; Setty, Swati; Thakur, Srinath

doi:10.1902/jop.2012.110461

Cited by 27 publications

(22 citation statements)

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“…A few clinical studies have reported reduction in plaque accumulation and gingival bleeding [26, 30] and decreased proportion of mutans streptococci [31] with application of honey intraorally, supporting the use of honey as an oral antimicrobial agent. Thus, the present preclinical study assessed the potential for subgingival application of manuka honey in the treatment of periodontal diseases.…”

Section: Discussionmentioning

confidence: 99%

Periodontal Application of Manuka Honey: Antimicrobial and Demineralising Effects In Vitro

Safii

Tompkins

Duncan

2017

International Journal of Dentistry

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Background. Topical application of manuka honey is effective in the treatment of burns and soft-tissue infections. The aim of this study was to assess the antibacterial activity of manuka honey against plaque-associated bacteria in vitro in order to evaluate the potential application as an adjunct to periodontal treatment. Materials and Methods. The minimum bacteriostatic and bactericidal concentrations (MIC and MBC) of manuka honey were compared to those of white clover honey against a variety of plaque-associated bacteria, at the natural and neutral pH. Dissolved calcium was measured following incubation of honeys with hydroxyapatite (HA) beads to assess their potential to demineralise oral hard tissues. Results. Both honeys inhibited most tested oral bacteria at similar MIC/MBC, but Streptococcus mutans was comparatively resistant. The honeys at pH neutral had little effect on antimicrobial activity. Incubation of HA beads in honey solutions resulted in pH-dependent calcium dissolution, and inoculation with S. mutans promoted further demineralisation by both types of honey. Conclusion. Manuka honey is antimicrobial towards representative oral bacteria. However, the relative resistance of S. mutans in association with the high concentrations of fermentable carbohydrates in honey and the direct demineralising effect at natural pH mitigate against the application of honey as an adjunct in the treatment of periodontal disease.

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Section: Discussionmentioning

confidence: 99%

Periodontal Application of Manuka Honey: Antimicrobial and Demineralising Effects In Vitro

Safii

Tompkins

Duncan

2017

International Journal of Dentistry

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“…In an in vitro study it was shown that chlorhexidine acts more antibacterial than honey [48]. However, the addition of honey or its components as natural products to mouth rinses, tooth pastes may be discussed as a beneficial option in prevention and therapy of periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Honey – a potential agent against Porphyromonas gingivalis: an in vitro study

et al. 2014

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BackgroundHoney has been discussed as a therapeutic option in wound healing since ancient time. It might be also an alternative to the commonly used antimicrobials in periodontitis treatment. The in-vitro study was aimed to determine the antimicrobial efficacy against Porphyromonas gingivalis as a major periodontopathogen.MethodsOne Manuka and one domestic beekeeper honey have been selected for the study. As a screening, MICs of the honeys against 20 P. gingivalis strains were determined. Contents of methylglyoxal and hydrogen peroxide as the potential antimicrobial compounds were determined. These components (up to 100 mg/l), propolis (up to 200 mg/l) as well as the two honeys (up to 10% w/v) were tested against four P. gingivalis strains in planktonic growth and in a single-species biofilm.Results2% of Manuka honey inhibited the growth of 50% of the planktonic P. gingivalis, the respective MIC50 of the German beekeeper honey was 5%. Manuka honey contained 1.87 mg/kg hydrogen peroxide and the domestic honey 3.74 mg/kg. The amount of methylglyoxal was found to be 2 mg/kg in the domestic honey and 982 mg/kg in the Manuka honey. MICs for hydrogen peroxide were 10 mg/l - 100 mg/l, for methylglyoxal 5 – 20 mg/l, and for propolis 20 mg/l – 200 mg/l. 10% of both types of honey inhibited the formation of P. gingivalis biofilms and reduced the numbers of viable bacteria within 42 h-old biofilms. Neither a total prevention of biofilm formation nor a complete eradication of a 42 h-old biofilm by any of the tested compounds and the honeys were found.ConclusionsHoney acts antibacterial against P. gingivalis. The observed pronounced effects of Manuka honey against planktonic bacteria but not within biofilm can be attributed to methylglyoxal as the characteristic antimicrobial component.

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“…We used a non-brushing model that allowed for dental plaque accumulation that has been widely used to evaluate the effects of various mouthrinses [ 19 – 22 , 26 – 28 ]. Conducted in the absence of mechanical oral hygiene, the method provides direct evidence of the chemical action of formulations against plaque growth [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of a mouthrinse containing rice peptide CL(14-25) on early dental plaque regrowth: a randomized crossover pilot study

et al. 2015

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BackgroundWe aimed to evaluate clinically the effect of mouthrinse containing a rice peptide on early dental plaque regrowth.MethodsThe study was designed as a double-masked, two-group crossover randomized pilot trial, involving 10 periodontally healthy volunteers. After receiving a professional tooth cleaning at baseline, over the next 3 days each participant refrained from all oral hygiene measures and had two daily rinses with 20 ml of the test mouthrinse containing 0.4 % rice peptide CL(14-25) or placebo rinse. At the end of each experimental period, plaque score was assessed using the modified Volpe’s method, and the participants filled out a questionnaire. Each participant underwent a 7-day washout period followed by a second allocation. The plaque score was the primary outcome of the study and participant perception was the secondary outcome.ResultsNo adverse effects were observed in the participants during the study. Clinically, the mean plaque score of the examined teeth was significantly lower in the test group (2.44 ± 0.74, CI: 1.91–2.96) than the placebo group (2.65 ± 0.63, CI: 2.20–3.10) (P < 0.05). When analyzed according to the type of teeth, a significantly lower score of the premolars/molars was observed in the test group (2.39 ± 0.68, CI: 2.08–2.71) than that in the placebo group (2.66 ± 0.58, CI: 2.39–2.93) (P < 0.05).ConclusionsThe mouthrinse containing 0.4 % rice peptide CL(14-25) was effective in reducing the early regrowth of dental plaque. However, clinical relevance of this efficacy needs to be validated in a future large-scale study.Trial registration: UMIN Clinical Trials Registry (UMIN-CTR) R000014000. Date of formal registration: November 1, 2013.

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A Comparative Evaluation of the Antibacterial Efficacy of Honey In Vitro and Antiplaque Efficacy in a 4‐Day Plaque Regrowth Model In Vivo: Preliminary Results

Abstract: Honey has antibacterial action against tested oral microorganisms and also has antiplaque action.

Cited by 27 publications

References 44 publications

Periodontal Application of Manuka Honey: Antimicrobial and Demineralising Effects In Vitro

Periodontal Application of Manuka Honey: Antimicrobial and Demineralising Effects In Vitro

Honey – a potential agent against Porphyromonas gingivalis: an in vitro study

Effect of a mouthrinse containing rice peptide CL(14-25) on early dental plaque regrowth: a randomized crossover pilot study

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