A comparative evaluation of photo-toxic effect of fractionated melanin and chlorpromazine hydrochloride on human (dermal fibroblasts and epidermal keratinocytes) and mouse cell line/s (fibroblast Balb/c 3T3)

Rai, Vishwas; Dayan, Nava; Michniak‐Kohn, Bozena

doi:10.1016/j.tiv.2010.11.017

Cited by 12 publications

(11 citation statements)

References 18 publications

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“…However, there has been no confirmation that these results can be reproduced in a human cell line using the same protocols. In addition, Rai et al proposed that human cell lines are preferable to the mouse fibroblast cell line when evaluating phototoxicity. Assay sensitivity and behavior may therefore differ between mouse and human cells.…”

Section: Introductionmentioning

confidence: 99%

Safety and efficacy of tyrosinase inhibition of Paeonia suffruticosa Andrews extracts on human melanoma cells

Lin

Wang

Song

et al. 2019

J of Cosmetic Dermatology

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Introduction The development of tyrosinase inhibitors is a hot research topic. Recently, the Chinese herb Paeonia suffruticosa Andrews, commonly named as Cortex Moutan (CM), was reported as being capable of reducing melanogenesis. We developed an A2058 human melanoma cell model to test the safety and efficacy of tyrosinase inhibition. The aim was to further clarify the bioactivities of CM extracts and paeonol for the purpose of skin whitening. Methods The 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) scavenging activity, total polyphenol and flavonoid contents, and in vitro tyrosinase inhibitory effects of water and ethanol CM extracts were determined. Cellular inhibitions of tyrosinase and melanin production were also evaluated. Results Water and ethanol CM extracts were both shown to have strong DPPH scavenging abilities in a dose‐dependent manner. The polyphenol content was higher in the ethanol CM extract compared to the water extract, while the flavanone content was comparable. Kinetic analyses revealed that the ethanol CM extract and paeonol are noncompetitive tyrosinase inhibitors. The cellular melanin content and l‐DOPA oxidation assays demonstrated that the ethanol CM extract was an appropriate alternative whitening agent to paeonol and arbutin in ultraviolet‐induced A2058 human melanoma cells. Conclusion The results of this study suggest that a human cell model is more suitable for determining tyrosinase activity than mouse cell models for determining cellular tyrosinase activity and melanin production. The ethanol CM extract was also confirmed as a promising ingredient in sun protection and skin whitening cosmetics. Future work should focus on melanogenesis‐related gene expressions.

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Section: Introductionmentioning

confidence: 99%

Safety and efficacy of tyrosinase inhibition of Paeonia suffruticosa Andrews extracts on human melanoma cells

Lin

Wang

Song

et al. 2019

J of Cosmetic Dermatology

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“…Determination of Phototoxicity. Phototoxicity was evaluated using RGO and CPZ on the mouse fibroblast cell line Balb/c 3T3 using a previously described method [21]. Cells were seeded in two 96-well plates with (+UV) and without (−UV) UV light for RGO and CPZ at a density of 1 × 10 4 cells/well.…”

Section: Measurement Of Serum Markers For Oxidative Stressmentioning

confidence: 99%

Red Ginseng Oil Attenuates Oxidative Stress and Offers Protection against Ultraviolet‐Induced Photo Toxicity

Ullah

Lee

Kim

et al. 2021

Oxidative Medicine and Cellular Longevity

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Ginseng (Panax ginseng Meyer) is a well-known herbal medicine that has been used for a long time in Korea to treat various diseases. This study investigated the in vitro and in vivo protective effects of red ginseng extract (RGE) and red ginseng oil (RGO). Liver injury was produced in BALB/c mice by 400 mg/kg of acetaminophen intraperitoneal injection. The antioxidant effects of RGE and RGO on the free radicals 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2 ′ -azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) were measured. In addition, the hepatoprotective activities of RGE and RGO on liver markers, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), and oxidative stress markers, including superoxide dismutase (SOD), catalase (CAT) enzyme activity, and 8-hydroxy-2-deoxyguanosine (8-OHdG) in serum and histopathological analysis, were evaluated. The protective effect of RGO on UV-induced phototoxicity was also evaluated in Balb/c 3T3 mouse fibroblast cell line. RGE and RGO effectively inhibited the radicals DPPH and ABTS compared with ascorbic acid and trolox, respectively. Moreover, RGE and RGO significantly decreased the liver enzyme (ALT and AST) levels, increased the antioxidant enzyme (SOD and CAT) levels, and decreased the DNA oxidation product (8-OHdG) content in mice serum. RGO also exhibited protective effect against UV irradiation compared with chlorpromazine hydrochloride, a known phototoxic drug, in Balb/c 3T3 cell line. RGE and RGO possess antioxidant and hepatoprotective properties in mice, and RGO exerts nonphototoxic activity in Balb/c 3T3 cells.

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“…The term High-Energy Visible (HEV) light is sometimes used in lieu of the blue light hazard but tends to refer to the same wavebands. HEV ranges fluctuate from 380 to 500 nm [23], 400-500 nm [24], and others stating 430-510 nm [25] depending on the author's particular field. This is different from the "Retinal Hazard Region" often defined as 400-1400 nm [26].…”

Section: Spectral Band Terminologymentioning

confidence: 99%

Photobiomodulation of the Visual System and Human Health

Buch

Hammond

2020

IJMS

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Humans express an expansive and detailed response to wavelength differences within the electromagnetic (EM) spectrum. This is most clearly manifest, and most studied, with respect to a relatively small range of electromagnetic radiation that includes the visible wavelengths with abutting ultraviolet and infrared, and mostly with respect to the visual system. Many aspects of our biology, however, respond to wavelength differences over a wide range of the EM spectrum. Further, humans are now exposed to a variety of modern lighting situations that has, effectively, increased our exposure to wavelengths that were once likely minimal (e.g., “blue” light from devices at night). This paper reviews some of those biological effects with a focus on visual function and to a lesser extent, other body systems.

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A comparative evaluation of photo-toxic effect of fractionated melanin and chlorpromazine hydrochloride on human (dermal fibroblasts and epidermal keratinocytes) and mouse cell line/s (fibroblast Balb/c 3T3)

Cited by 12 publications

References 18 publications

Safety and efficacy of tyrosinase inhibition of Paeonia suffruticosa Andrews extracts on human melanoma cells

Safety and efficacy of tyrosinase inhibition of Paeonia suffruticosa Andrews extracts on human melanoma cells

Red Ginseng Oil Attenuates Oxidative Stress and Offers Protection against Ultraviolet‐Induced Photo Toxicity

Photobiomodulation of the Visual System and Human Health

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