A comparative evaluation of phenotypic and molecular methods in the identification of members of the Staphylococcus sciuri group

Stepanović, Srdjan; Dakić, Ivana; Martel, An; Vaneechoutte, Mario; Morrison, Donald A.; Shittu, Adebayo; Ježek, Petr; Decostere, Annemie; Devriese, Luc; Haesebrouck, Freddy

doi:10.1016/j.syapm.2005.02.001

Cited by 22 publications

(13 citation statements)

References 36 publications

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“…2004) was used for the identification of methicillin‐resistant strains as members of the genus Staphylococcus and their identification to the species level, but only for S. aureus , Staphylococcus epidermidis , Staphylococcus saprophyticus and Staphylococcus xylosus . Identification of Staphylococcus sciuiri isolates was based upon phenotypic traits (Stepanovic et al . 2005, 2007).…”

Section: Susceptibility Of Methicillin‐resistant Coagulase‐negative mentioning

confidence: 99%

Public transport as a reservoir of methicillin-resistant staphylococci

Stepanović¹,

Ćirković²,

Djukić³

et al. 2008

Letters in Applied Microbiology

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Section: Susceptibility Of Methicillin‐resistant Coagulase‐negative mentioning

confidence: 99%

Public transport as a reservoir of methicillin-resistant staphylococci

Stepanović¹,

Ćirković²,

Djukić³

et al. 2008

Letters in Applied Microbiology

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“…Identification of the isolates to the species level by phenotypic methods was achieved by a previously described protocol. 31 …”

Section: Bacterial Strainsmentioning

confidence: 99%

Aminoglycoside Resistance in Members of theStaphylococcus sciuriGroup

Hauschild

Vuković

Dakić

et al. 2007

Microbial Drug Resistance

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This study investigated the prevalence of aminoglycoside resistance and genes encoding aminoglycoside-modifying enzymes in members of the Staphylococcus sciuri group. A total of 304 S. sciuri group member isolates (284 S. sciuri, 12 S. lentus, and 8 S. vitulinus) from humans (n = 34), animals (n = 133), and environmental sources (n = 137; out-hospital and hospital environment, food) were examined for their susceptibility to amikacin, gentamicin, isepamicin, kanamycin, neomycin, netilmicin, sisomicin, streptomycin, and tobramycin. The overall prevalence of resistance to aminoglycosides was low at 12.1%. Resistance to single aminoglycosides ranged from 0% to 7.2%. The aac(6')-Ie/aph(2"), ant(4')-Ia, and aph(3')-IIIa genes, either alone or in combination, were found in 16 out of 19 isolates showing resistance to nonstreptomycin aminoglycosides. Among the 22 isolates that showed resistance to streptomycin, the genes str and ant(6)-Ia were identified in 18 and 4 isolates, respectively.

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“…rodentium , Staphylococcus sciuri subsp. sciuri , Staphylococcus lentus , and Staphylococcus vitulinus [37], [38], more efforts will be required to elucidate the genotype of this isolate.…”

Section: Discussionmentioning

confidence: 99%

A Highly Pathogenic Strain of Staphylococcus sciuri Caused Fatal Exudative Epidermitis in Piglets

et al. 2007

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Staphylococcus sciuri are important human pathogens responsible for endocarditis, peritonitis, septic shock, urinary tract infection, pelvic inflammatory disease and wound infections. However, little information is known regarding the pathogenicity of S. sciuri to animals. From the pericardial fluid of a diseased piglet with exudative epidermitis (EE), we isolated a strain of Staphylococcus in pure culture. Surprisingly, this isolate was a member of S. sciuri rather than S. hyicus as identified by its biochemical traits and also by analysis of 23S ribosomal DNA using Internal Transcribed Spacer PCR. In addition, inoculation of newborn piglets with 1×1010 CFU of the isolate by oral feeding or intra-muscular injection successfully reproduced EE in piglets, which suggested that the oral intake of the pathogen by the animals is one of the major routes of exposure. These unexpected findings prioritized S. sciuri as important zoonotic agents, which may have ramifications for human medicine.

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A comparative evaluation of phenotypic and molecular methods in the identification of members of the Staphylococcus sciuri group

Cited by 22 publications

References 36 publications

Public transport as a reservoir of methicillin-resistant staphylococci

Public transport as a reservoir of methicillin-resistant staphylococci

Aminoglycoside Resistance in Members of theStaphylococcus sciuriGroup

A Highly Pathogenic Strain of Staphylococcus sciuri Caused Fatal Exudative Epidermitis in Piglets

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