A comparative evaluation of layer‐by‐layer assembly techniques for surface modification of microcarriers used in human mesenchymal stromal cell manufacturing

Abstract

The demand for large quantities of highly potent human mesenchymal stromal cells (hMSCs) is growing given their therapeutic potential. To meet high production needs, suspension‐based cell cultures using microcarriers are commonly used. Microcarriers are commonly made of or coated with extracellular matrix proteins or charged compounds to promote cell adhesion and proliferation. In this work, a simple method (draining filter) to perform layer by layer (LbL) assembly on microcarriers to create multilayers of heparin and collagen and further demonstrate that these multilayers have a positive effect on hMSC viability after 48 h of culture was demonstrated. The draining filter method is evaluated against two other methods found in literature—centrifugation and fluidized bed, showing that the draining filter method can perform the surface modification with greater efficiency and with less materials and steps needed in the coating process.

Identification of critical process parameters for expansion of clinical grade human Wharton's jelly‐derived mesenchymal stromal cells in stirred‐tank bioreactors

“…Seeding therefore requires optimization when transitioning from 2D to microcarrier-based cultures, considering options such as refinements to the medium composition and microcarrier coating. [28,29] The majority of studies conducted to date refer to large-scale expansion of MSCs from bone marrow and adipose tissue sources, [30] and only a few have reported the expansion of WJ-MSCs on microcarriers in spinner flasks [17,18,22,[31][32][33] or STRs [17,18,20,31,34] using different experimental approaches based mainly on the use of hPL and SCM.…”