A comparative evaluation of disinfection effect of exposures to ultra-violet light and direct current glow discharge on Candida Albicans colonies coated over elastomeric impression material: An in vitro study

Abstract

Aim:The aim of our study is to compare the efficacy of ultra-violet light (U-V light) and direct current glow discharge in disinfecting Candida Albicans coated elastomeric impression material.Materials and Methods:Two hundred and forty samples of addition silicone material in the form of circular discs measuring (diameter-30 mm, thickness-3 mm) were prepared. Samples were divided into four groups namely A, B, C, D, with each group containing 60 samples. All samples in each group were sub grouped as follows for exposure time 15, 30, 60, 90, 120 and 180 s respectively. Group A samples were exposed to U-V light with 8 watts. Group B samples were exposed to U-V light with 16 watts. Group C samples were exposed to U-V light with 24 watts. Group D samples were exposed to direct current glow discharge. After exposure, the impression material was swabbed on sabourauds dextrose agar (SDA) plates and incubated at 37°C for 48 h. The total number of colonies indicating the number of C. Albicans that survived the direct current glow discharge and U-V light treatment was then determined using a microscope.Results and Conclusion:Group A samples exhibited proportionate decrease in the number of colonies with each greater time of exposure. Group B samples exhibited proportionate decrease in the number of colonies with each greater time of exposure. Group C samples exhibited total absence of C. Albicans colonies at 90 s exposure. In Group D samples there was a proportionate decrease in number of C. Albicans colonies with exposure to direct current glow discharge for more seconds. Hence, this study reveals that exposure to U-V light drastically reduced the C. Albicans colonies compared with exposure to direct current glow discharge. It was observed that with greater wattage of U-V light tube in U-V light unit chamber, greater decrease in colony count was observed in lesser time of exposure.

Comprehensive Review and Comparison of the Disinfection Techniques Currently Available in the Literature

“…The direct‐current glow discharge sustains the discharges in the range of milliamps to tens of milliamps. Direct‐current glow discharge has been shown to decrease the count of C. albicans colonies, but UV light could be a better disinfection method than direct‐current glow discharge …”

Radiofrequency Glow Discharge as a Mode of Disinfection for Elastomeric Impression Materials

“…Energy is generated during the glow discharge/plasma cleaning procedure, which could clean the surface being exposed. 32,33 This has been proved to be useful in the sterilization of implants and various studies supporting the same have been reported. Radiofrequency glow discharge is much handy and convenient procedure than immersion disinfection and spray atomization, which are the most commonly used modes of disinfection for impression materials.…”

Prosthetic impressions disinfectants and evaluation of their effectiveness – review of the literature

“…Możliwość zarażenia chorobą zakaźną pracowników stomatologicznych wszystkich szczebli, a także pacjentów, wymogła konieczność stosowania skutecznych środków i metod dezynfekcji, tak aby spełniona była jedna z fundamentalnych zasad medycyny: primum non nocere. 1,2 Wyniki najnowszych badań dowodzą, że 67% materiałów przekazywanych do laboratorium protetycznego jest zainfekowanych mikroorganizmami. Najczęściej izolowanymi patogenami są: Streptococcus sp; Staphylococcus sp; Escherichia coli sp; Actinomyces sp; Antitratus sp; Pseudomonas sp; Enterobacter sp; Klebsiella pneumoniae i Candida sp.…”

“…Promieniowanie to jest niewidzialne dla człowieka, a jego nazwa pochodzi z łacińskiego słowa ultra (ponad, poza) oraz od słowa "fiolet", oznaczającego barwę o najmniejszej długości fali w świetle widzialnym. Ze względu na skutki działania na organizmy żywe, promieniowanie ultrafioletowe dzieli się na trzy typy: UV-A, UV-B oraz UV-C. 1,25,28,31 W stomatologii ultrafiolet może być stosowany jako metoda dezynfekcji wycisków. Coraz liczniejsze badania naukowe dowodzą jego skuteczności w tym zakresie.…”