A Comparative Evaluation of Concentrated Growth Factor and Platelet-rich Fibrin on the Proliferation, Migration, and Differentiation of Human Stem Cells of the Apical Papilla

Hong, Shebin; Chen, Weiting; Jiang, Beizhan

doi:10.1016/j.joen.2018.03.006

Cited by 58 publications

(55 citation statements)

References 37 publications

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“…Previous studies have evaluated the biological effects of CGF and PRF on the SCAPs, and the results revealed that both CGF and PRF can promote the proliferation, migration and differentiation of SCAPs (Hong et al . ). Although the data did not reveal significant differences between CGF and PRF, the improved CGF procedure could optimize the amount of growth factors (Rodella et al .…”

Section: Discussionmentioning

confidence: 97%

“…Though previous studies have compared the biological differences between CGF and PRF (Hong et al . ), the structure and cytokines in CGF have not been well investigated.…”

Section: Discussionmentioning

confidence: 99%

“…To achieve successful pulp regeneration through cell homing, a suitable combination of scaffold and growth factors should be selected. Though previous studies have compared the biological differences between CGF and PRF (Hong et al 2018), the structure and cytokines in CGF have not been well investigated. In this study, the histologic staining and SEM evaluation revealed that abundant erythrocytes, leucocytes and platelets were concentrated in the lower part of the CGF clot, and the acellular component, including the cross-linked fibrin and the trapped platelets, was in the upper region.…”

Section: Discussionmentioning

confidence: 99%

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The potential application of concentrated growth factor in regenerative endodontics

Hong

Cai

et al. 2018

Int Endodontic J

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Aim To investigate the potential application of concentrated growth factor (CGF) to promote pulp regeneration within immature teeth. Methodology Concentrated growth factor clots produced from peripheral blood samples were investigated histologically by haematoxylin–eosin (HE) staining and evaluated morphologically by scanning electron microscope (SEM). The cytokines were extracted from the CGF, and representative cytokines were quantified by enzyme‐linked immunosorbent assay (ELISA). The biological effects of the CGF on human stem cells from the apical papilla (SCAPs) were then investigated and quantified, including cell proliferation, cell migration, mineralized nodule formation, and the gene expression of alkaline phosphatase (ALP), dentine sialophosphoprotein (DSPP) and dentine matrix protein (DMP)‐1. The results were analysed statistically using one‐way analysis of variance (anova). Results Concentrated growth factor had a complex three‐dimensional structure with a high density of platelets and nucleated cells. Representative growth factors including PDGF‐BB, IGF‐1, TGF‐β1, bFGF and VEGF were detected. The growth rate and migratory cell numbers of the CGF groups were significantly greater than those in the control groups (P < 0.05). The mineralization areas in the CGF groups were significantly larger than those in the control groups (P < 0.05). The expression levels of ALP, DSPP and DMP‐1 were significantly up‐regulated after induction by CGF (P < 0.05). Conclusions Concentrated growth factor promoted the proliferation, migration and differentiation of SCAPs and could be a promising biomaterial applied in regenerative endodontics.

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Section: Discussionmentioning

confidence: 97%

“…Though previous studies have compared the biological differences between CGF and PRF (Hong et al . ), the structure and cytokines in CGF have not been well investigated.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The potential application of concentrated growth factor in regenerative endodontics

Hong

Cai

et al. 2018

Int Endodontic J

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“…Although under treatment with CGF extracts, higher cells proliferation in a dose‐dependent manner was approved by Jin et al, enhanced cell migration, and differentiation in the low doses (<50%) of CGF was noticeable (Jin et al, ). Two studies compared effect of CGF with PRF, and the results show that PRF is more efficient in osteogenic differentiation of stem cells, while CGF is more efficient in vascular differentiation (Hong et al, ; Hu et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…However, four studies showed that a high dose of CGF does not have a positive effect. In more efficient in vascular differentiation (Hong et al, 2018;Hu et al, 2018).…”

Section: Effect Of Cgf On Stem Cellsmentioning

confidence: 99%

In vitro and in vivo effects of concentrated growth factor on cells and tissues

Tabatabaei

Aghamohammadi

Tayebi

2020

J Biomedical Materials Res

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This article reviews the biological outcome of the concentrated growth factor (CGF), a new platelet derivative used for tissue regeneration, in published articles related to the use of this product in basic and clinical studies. An electronic literature research using PubMed and SCOPUS was performed using combination of keywords: “concentrated growth factor” (OR “CGF”), AND “stem cells,” AND “cells” OR “cell proliferation” OR “cell migration” OR “cell differentiation,” AND “repair” OR “survival” OR “revitalization,” AND “tissue” OR “bone.” Forty‐five articles that were published between 2012 and 2020 met the inclusion criteria. These studies have used CGF as fresh solid form, freeze‐dried, membrane, extract, or exudate. Most studies demonstrate the positive effects of CGF in a dose‐dependent manner under certain concentrations. Studies comparing CGF with other platelet concentrates, report lower efficiency, no statistically significant differences, or better results for CGF. Combination of CGF with stem cells and biomaterials significantly improves bone regeneration and the effect of allograft or collagen membrane is better than CGF alone. For a better examination of the biological outcomes of CGF, the standardization of CGF preparation regarding the choice of the test tube material for blood collection, the required volume of blood, the necessary count of platelets in CGF, and the most appropriate type of CGF are recommended.

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Sodium fluoride regulates the osteo/odontogenic differentiation of stem cells from apical papilla by modulating autophagy

Pan

Wang

et al. 2019

Journal Cellular Physiology

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Fluoride (sodium fluoride) is thought to be essential in the development of tooth, and research shows that fluoride can modulate the differentiation of dental stem cells. However, the effects of fluoride on the committed differentiation of stem cells from apical papilla (SCAPs) and the underlying mechanisms remain unclear. Here, SCAPs were isolated from healthy extracted human third molars with immature roots and then were cultured with NaF conditioned media. Cell Counting Kit‐8, EdU staining, and flow cytometry were performed to detected the proliferation activity. Alkaline phosphatase (ALP) activity, Alizarin Red staining, Western blot assay, and real‐time reverse‐transcription polymerase chain reaction were applied to assess the osteo/odontogenic differentiation NaF‐treated SCAPs. Western blot assay and transmission electron microscope were used to evaluate the autophagy involved in the differentiation of SCAPs. ALP activity, ALP protein, and messenger RNA (mRNA) expression showed that 0.5 mM was the optimal concentration for the induction of SCAPs by NaF. 0.5 mM NaF‐treated SCAPs induced more mineralized nodules as compared with untreated cells. Moreover, the osteo/odontogenic markers (RUNX2, OSX, DSP, and OCN) in mRNA levels were upregulated while the protein levels of these markers increased considerably in 0.5 mM NaF‐treated SCAPs. Furthermore, the autophagy‐related proteins (LC3, ATG5, and Beclin1) increased in NaF‐treated SCAPs, and the osteo/odontogenic makers significantly decreased while silencing ATG5 to block autophagy. In all, sodium fluoride can regulate the osteo/odontogenic differentiation of SCAPs by modulating autophagy.

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A Comparative Evaluation of Concentrated Growth Factor and Platelet-rich Fibrin on the Proliferation, Migration, and Differentiation of Human Stem Cells of the Apical Papilla

Cited by 58 publications

References 37 publications

The potential application of concentrated growth factor in regenerative endodontics

The potential application of concentrated growth factor in regenerative endodontics

In vitro and in vivo effects of concentrated growth factor on cells and tissues

Sodium fluoride regulates the osteo/odontogenic differentiation of stem cells from apical papilla by modulating autophagy

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