A comparative evaluation of antimicrobial efficacy of novel surfactant-based endodontic irrigant Regimen's on Enterococcus faecalis

Ravinanthanan, Manikandan; Hegde, Mithra N; Shetty, Veena; Kumari, Suchetha; Qahtani, Fahd Nasser Al

doi:10.4103/ccd.ccd_360_20

Cited by 2 publications

(6 citation statements)

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“…This method measures the effect of direct and close contact between a fixed volume of the antimicrobial agent and a fixed volume of a microbial suspension for a particular time [ 2 ]. The antimicrobial activities of the used irrigants in this study were assessed after 5 min because MTAD, according to the manufacturer's instructions, requires a contact time of 5 min [ 1 ].…”

Section: Discussionmentioning

confidence: 99%

“…Isolated colonies of these microorganisms were suspended in a sterile saline (0.9% NaCl) solution after being grown aerobically in BHI broth for 24 hr. The cell suspension was adjusted spectrophotometrically at 600 nm to match the turbidity of 0.5 McFarland scale (1.5 × 10 8 colony-forming units (CFU)/mL) [ 1 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…This in vitro study was carried out at the educational laboratories of the College of Health and Medical Technologies, Baghdad. One milliliter of each microbial suspension was obtained and added to a tube containing 1 mL of the irrigant solutions (2% CHX, 0.2% CPC + 2% CHX, and 100% MTAD) for the experimental time of 5 min [ 1 , 21 ]. After the indicated exposure time, 100 µ L samples were obtained, added to 900 µ L of sterile saline contained in Eppendorf tubes, and serially diluted.…”

Section: Methodsmentioning

confidence: 99%

“…Finally, droplets of 10 µ L from the diluted and undiluted tubes were cultured on Mueller–Hinton agar plates and incubated at 37°C for 24 hr for colony counting under magnification using a colony-counting device (Suntex, Taiwan). The procedure was performed ten times for each subgroup [ 1 , 21 , 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…The main goal of the endodontic therapy is to provide a biologically suitable condition inside the root canal system that permits healing and ongoing maintenance of the periradicular tissue's health [ 1 ]. Therefore, complete debridement and effective elimination of microbiological irritants, including microorganisms and their byproducts, followed by obturation of the entire root canal system, are necessary for successful endodontic therapy [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Addition of Cetylpyridinium Chloride Cationic Surfactant on the Antimicrobial Activity of Chlorhexidine Endodontic Irrigant

Al-Sada,

Al-Gharrawi

2024

International Journal of Dentistry

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Background. Endodontic irrigants are essential for disinfecting the root canal system. None of the currently available irrigants perfume sufficiently. However, most products contain surfactants, which enhance the antimicrobial properties of the irrigants. Objectives. To evaluate the effect of cetylpyridinium chloride (CPC) surfactant on the antimicrobial activity of chlorhexidine and compare it with that of chlorhexidine (CHX) and Biopure MTAD against Enterococcus faecalis, Staphylococcus aureus, and Candida albicans. Materials and Methods. In this in vitro study, three microorganisms were used (E. faecalis, S. aureus, and C. albicans), and each organism was treated with three different irrigants: 2% CHX, 2% CHX + 0.2% CPC, and 100% Biopure MTAD. The antimicrobial activity was evaluated by direct contact assay for 5 min of contact time. The colony-forming unit per mL was calculated after antimicrobial treatment and 24 hr of incubation at 37°C. The data were statistically analyzed using Statistical Package for Social Science (SPSS) version 22. The Kruskal–Wallis and the multiple Wilcoxon sum rank tests were used. The level of significance was set at 0.05. Results. The result showed a nonsignificant difference between the different irrigants against E. faecalis. Among S. aureus subgroups, 2% CHX was statistically significant and more efficient than MTAD. Among C. albicans subgroups, 2% CHX and combined irrigant (2% CHX + 0.2% CPC) were statistically more efficient than MTAD. The 2% CHX and combined irrigants were equally effective against all the tested microorganisms. Conclusions. All the used irrigants have comparable effects against E. faecalis after 5 min. CHX have a comparable effects to that of the combined irrigant and more efficient against S. aureus than MTAD. CHX and the combined irrigant have potent antimicrobial activity against C. albicans superior to MTAD. CPC surfactant can be used with CHX to overcome its clinical drawbacks or limitations without altering or reducing its antimicrobial activity.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%