A comparative cytogenetic study of miscarriages after IVF and natural conception in women aged under and over 35 years

Pendina, Anna A.; Efimova, Olga A.; Chiryaeva, Olga G.; Tikhonov, Andrei V.; Petrova, L; Dudkina, Vera S.; Sadik, N. Al; Федорова, И. М.; Galembo, Ilona A.; Kuznetzova, T. V.; Gzgzyan, Alexander M.; Baranov, V. S.

doi:10.1007/s10815-013-0148-1

Cited by 29 publications

(22 citation statements)

References 32 publications

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“…Невынашивание беременности -одно из частых ос-ложнений вспомогательных репродуктивных технологий, по результатам проведенного исследования составляет 43%, что соответствует данным публикаций других иссле-дователей [23]. Спонтанный аборт можно рассматривать как естественный способ отбора жизнеспособного потом-ства, который тем не менее является небезопасным для женщины и приводит к существенным финансовым за-тратам и эмоциональному стрессу пациентки.…”

Section: Discussionunclassified

Cytogenetic causes of miscarriage induced in an in vitro fertilization program

Sinkov¹,

Motyreva²,

Serkova³

et al. 2016

Ross. vestn. akush.-ginekol.

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Section: Discussionunclassified

Cytogenetic causes of miscarriage induced in an in vitro fertilization program

Sinkov¹,

Motyreva²,

Serkova³

et al. 2016

Ross. vestn. akush.-ginekol.

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“…Metaphase chromosomes and interphase nuclei from the chorionic villi and embryonic lung samples were prepared by the direct technique (without culturing) according to the protocol described previously [Baranov et al, 1990] with modifications [Pendina et al, 2014]. Briefly, the tissue samples were dissected into small fragments and treated with hypotonic solution (0.9% sodium citrate) supplemented with colchicine (final concentration 2.5 μg/ mL) for 60-90 min at room temperature.…”

Section: Slide Preparation and Immunodetection Of 5hmc And 5mcmentioning

confidence: 99%

Inter-Cell and Inter-Chromosome Variability of 5-Hydroxymethylcytosine Patterns in Noncultured Human Embryonic and Extraembryonic Cells

Efimova

Pendina

Krapivin

et al. 2018

Cytogenet Genome Res

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5-hydroxymethylcytosine (5hmC) is an oxidative derivative of 5-methylcytosine (5mC). Recent studies have revealed a sharp difference in the levels of 5hmC in 2 opposite DNA strands of a given chromosome and a chromosome-wide cell-to-cell variability in mammalian cells. This asymmetric 5hmC distribution was found in cultured cells, which may not fully mimic in vivo epigenetic processes. We have checked whether inter-chromosome and inter-cell variability of 5hmC patterns is typical for noncultured human cells. Using indirect immunofluorescence, we analyzed the localization of 5hmC and its co-distribution with 5mC on direct preparations of mitotically active cells from human embryonic lung and chorionic cytotrophoblast samples. We demonstrated 3 types of chromosomes according to the 5hmC accumulation pattern: hydroxymethylated (5hmC in both sister chromatids), hemihydroxymethylated (5hmC in only 1 sister chromatid), and nonhydroxymethylated ones. Each accumulation type was not specific to any particular chromosome, resulting in different 5hmC patterns between homologous chromosomes, among chromosomes within each metaphase plate, among metaphases in one tissue, and between the tissues. The 5mC distribution was stable: chromosomes were methylated in R-bands and, especially in embryonic lung cells, in the heterochromatic regions 1q12, 9q12, and 16q11.2. Our results provide the first evidence of inter-cell and inter-chromosome variability of 5hmC patterns in human noncultured embryonic and extraembryonic cells.

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“…In general, the earlier in gestation the pregnancy is lost, the more likely chromosomal abnormality will be detected . However, in recurrent pre‐embryonic pregnancy loss, especially in subfertile young women (under 35 years), genetic abnormalities are signifficantly less frequent (9.1–29.8%), which indicates a causative role of other factors …”

Section: Genetic Abnormalities In First Trimester Pregnancy Lossmentioning

confidence: 99%

First trimester pregnancy loss: Clinical implications of genetic testing

Massalska

Zimowski

Bijok

et al. 2016

J of Obstet and Gynaecol

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Around 10-15% of pregnancies result in a spontaneous first trimester miscarriage, which is most frequently caused by chromosomal abnormalities, mainly aneuploidies. Genetic analysis of pregnancy loss includes conventional G-banding karyotyping and various molecular methods. Apart from variable methodological limitations, the effectiveness of genetic analysis depends on the type and quality of the tested sample. To improve the reliability of genetic testing, we present methods of appropriate collection and pre-laboratory preparation of chorionic villi from first trimester miscarriage. We also discuss issues of maternal cell contamination, placental mosaicism and reciprocal and Robertsonian translocations in the context of interpretation of the results and genetic counseling.

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A comparative cytogenetic study of miscarriages after IVF and natural conception in women aged under and over 35 years

Cited by 29 publications

References 32 publications

Cytogenetic causes of miscarriage induced in an in vitro fertilization program

Cytogenetic causes of miscarriage induced in an in vitro fertilization program

Inter-Cell and Inter-Chromosome Variability of 5-Hydroxymethylcytosine Patterns in Noncultured Human Embryonic and Extraembryonic Cells

First trimester pregnancy loss: Clinical implications of genetic testing

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