A comparative assessment of morphological and molecular diversity among Sechium edule (Jacq.) Sw. accessions in India

Abstract

Genetic variation of 36 Sechium edule accessions collected across 12 states in India was assessed using morphological traits and DAMD markers. Eighteen fruit morphological traits (both qualitative and quantitative) were evaluated to confirm the variations in the present collection. Quantitative traits showed major variations with respect to fruit weight (7.85-498.33 g/fruit), fruit length (5.8-15 cm/fruit), fruit diameter (6-28 cm/fruit) and length of the spine (0-5 cm). Qualitative traits were also diverse in fruit colour, shape, spine density, reticulation, flexibility of spine and furrow depth. The first six principle components showed 82.88% variation in the principal component analysis. The principal component analysis revealed that fruit weight, fruit width, fruit diameter, fruit shape, length of spine, spine density and furrow depth had a significant contribution to the total variation. The DNA analysis performed using DAMD primers were used for deducing the diversity at DNA level. The collection produced 102 bands out of which 97 were polymorphic and the percentage polymorphism ranged between 66.66 and 100 per primer. Discrete pattern of clustering was obtained using UPGMA method of complete linkage percent disagreement revealing high diversity among the collected accessions. Thus, the present study indicates that molecular and morphological marker map would improve our knowledge of S.edule and would facilitate efforts to breed improved S. edule cultivars.

Genetic diversity among melon ( Cucumis melo L.) accessions revealed by morphological traits and ISSR markers

“…We also believe that the sharing of breeding materials could be the main reason for low diversity between genotypes collected from distant geographic locations in our experiment. On the other hand, ISSR markers did not show a significant correlation with agromorphological data, which can be explained by the environmental factors as reported for several other crops (Kumar et al, 2014;Jain et al, 2017). However, overall, our results displayed a sufficient amount of morphological and genetic variability within the local melon germplasm (Figure 6).…”

Partial characterization of novel inulin‐like prebiotic fructooligosaccharides of Sechium edule (Jacq.) Sw. (Cucurbitaceae) tuberous roots

“…Swartz (Family Cucurbitaceae), commonly known as Chayote or Squash, a herbaceous perennial climbing plant with tuberous roots, is being cultivated since the pre‐Colombian time a wide range of climatic conditions in different continents (Lim, 2012). In India, the plant parts such as fruits, stems, and tender leaves are used as common cook vegetables in Nepal, Eastern Himalayas, Darjeeling district, and Tarai and Dooars regions of West Bengal, India (Jain et al, 2017; Lim, 2012). Most of the studies on the nutritional composition and the health benefits of this plant have mainly focused on the aerial parts but little on the tuberous roots (Hernandez Uribe et al., 2011; Hidalgo et al., 2016; Lombardo‐Earl et al, 2014; Shiga et al., 2015; Vieira et al., 2018).…”

“…Lalthansanga and Samanta (2015), in a recent study, reported that the whole plant feeding meal for 90 days in indigenous pig had improved the health status and Jain et al. (2017) documented the comparative assessment of morphological and molecular diversity among S. edule (Jacq.) Sw. accessions in India.…”

Analysis of genetic diversity among different Rose (Rosa) genotypes using morphological and molecular markers (ISSR)

“…These include morphological and molecular characterization, morphological traits are also essential for preliminary evaluation and for assessment of genetic diversity. Morphological characterization sets the basis for key traits of interest present in a germplasm collection as it is the simple way to assess variability in a collection (Khan et al, 2015;Jain et al, 2017).…”