A comparative assessment of DNA fingerprinting assays of ISSR and RAPD markers for molecular diversity of Saffron and other Crocus spp. in Iran

Zarini, Hamid Najafi; Jafari, Hoda; Ramandi, Hadi Darzi; Bolandi, A R; Karimishahri, Mahmoud Reza

doi:10.1007/s13237-018-0261-8

Cited by 14 publications

(2 citation statements)

References 31 publications

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“…Therefore, the DNA fingerprint variation using RAPD and ISSR markers has revealed that there exists a reasonable genetic difference among Crocus sativus L. ‘Kashmirianus’ samples collected from Jammu and Kashmir. The existence of genetic variation among Crocus sativus could be because of appropriate climatic conditions for its growth and development ( Larsen et al, 2015 , Najafi Zarini et al, 2019 , Nemati et al, 2014 ). The current research attempted to study molecular diversity of Crocus Sativus L. as an effort to contribute information to the branch of saffronology about the existence of molecular diversity within saffron cultivars of Jammu and Kashmir (India) along with selection of appropriate molecular characterization markers.…”

Section: Discussionmentioning

confidence: 99%

Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers

Mir

Mansoor

Sugapriya

et al. 2021

Saudi Journal of Biological Sciences

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The existence of genetic diversity in Crocus sativus has globally remained a mystery till date. The study investigated PCR based DNA amplification profile of saffron using ISSR and RAPD based primers. A total of 38 amplicons were generated by ISSR primers in the range from 7 to 12 with an average of 9.50 bands per primer. 20 bands were found to be polymorphic and 18 were monomorphic with an average percentage of polymorphism as 52.48%. RAPD based amplification revealed a total 161 amplicons, 107 as polymorphic and 54 as monomorphic with an average percentage of polymorphism as 66.44%. Cumulative results of RAPD and ISSR demonstrated that Nei-Li’s similarity index ranged between 0.70 and 0.97. The results of AMOVA has revealed 9% of variance among populations and 91% of variance within populations, Φ PT was found as 0.089, which indicates existence of genetic differences though limited. In conclusion, the results indicate that saffron accessions are minimally genetically differentiated, which could be capitalized in future breeding programmes to ameliorate this precious crop.

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Section: Discussionmentioning

confidence: 99%

Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers

Mir

Mansoor

Sugapriya

et al. 2021

Saudi Journal of Biological Sciences

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“…Both ISSR and SSR are widely used in plant breeding, e.g., for analyzing the genetic diversity of a population, for identifying the relationship of hybrids, QTL analyses, etc. [ 45 , 46 , 47 , 48 ]. Due to the fact that ISSRs are a universal marker system for species, they are widely used in loquat studies [ 49 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic Efficiency and Glyco-Metabolism Changes in Artificial Triploid Loquats Contribute to Heterosis Manifestation

Wang

MeiYan

et al. 2022

IJMS

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Previous studies indicated that extensive genetic variations could be generated due to polyploidy, which is considered to be closely associated with the manifestation of polyploid heterosis. Our previous studies confirmed that triploid loquats demonstrated significant heterosis, other than the ploidy effect, but the underlying mechanisms are largely unknown. This study aimed to overcome the narrow genetic distance of loquats, increase the genetic variation level of triploid loquats, and systematically illuminate the heterosis mechanisms of triploid loquats derived from two cross combinations. Here, inter-simple sequence repeats (ISSRs) and simple sequence repeats (SSRs) were adopted for evaluating the genetic diversity, and transcriptome sequencing (RNA-Seq) was performed to investigate gene expression as well as pathway changes in the triploids. We found that extensive genetic variations were produced during the formation of triploid loquats. The polymorphism ratios of ISSRs and SSRs were 43.75% and 19.32%, respectively, and almost all their markers had a PIC value higher than 0.5, suggesting that both ISSRs and SSRs could work well in loquat assisted breeding. Furthermore, our results revealed that by broadening the genetic distance between the parents, genetic variations in triploids could be promoted. Additionally, RNA-Seq results suggested that numerous genes differentially expressed between the triploids and parents were screened out. Moreover, KEGG analyses revealed that “photosynthetic efficiency” and “glyco-metabolism” were significantly changed in triploid loquats compared with the parents, which was consistent with the results of physiological indicator analyses, leaf micro-structure observations, and qRT-PCR validation. Collectively, our results suggested that extensive genetic variations occurred in the triploids and that the changes in the “photosynthetic efficiency” as well as “glyco-metabolism” of triploids might have further resulted in heterosis manifestation in the triploid loquats.

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Dendrobium sp.: In vitro Propagation of Genetically Stable Plants and Ethno-medicinal Uses

Tikendra¹,

Apana²,

Potshangbam³

et al. 2020

Reference Series in Phytochemistry

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A comparative assessment of DNA fingerprinting assays of ISSR and RAPD markers for molecular diversity of Saffron and other Crocus spp. in Iran

Cited by 14 publications

References 31 publications

Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers

Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers

Photosynthetic Efficiency and Glyco-Metabolism Changes in Artificial Triploid Loquats Contribute to Heterosis Manifestation

Dendrobium sp.: In vitro Propagation of Genetically Stable Plants and Ethno-medicinal Uses

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