A comparative assessment of culture and serology in the diagnosis of brucellosis in dairy cattle

O'Grady, Don; Byrne, William J.; Kelleher, Peter; O’Callaghan, H.; Kenny, Kevin; Heneghan, Terry; Power, Seamus; Egan, John; Ryan, F. B.

doi:10.1016/j.tvjl.2014.01.008

Cited by 25 publications

(19 citation statements)

References 28 publications

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“…Out of 626 test samples, 221(35.30%) and 247 (39.45%) were found positive by the RBPT and i-ELISA, respectively (Şahin et al 2008). O'Grady et al (2014) tested 248 bovine sera in Ireland and they found 38.7% and 16.1% positivity rates by i-ELISA and the RBPT, respectively. In another study conducted in 36 sheep flocks with history of abortion in Elazığ and its surrounding area, 500 serum samples were tested by i-ELISA, RBPT and CFT using B. abortus S99 as antigen and positivity rates were 20.6%, 11% and 17.8%, respectively (Öngör et al 2001).…”

Section: Discussionmentioning

confidence: 99%

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Comparative evaluation of three serological tests for the detection of Brucella antibodies from infected cattle herds

Tel

Keskin

2016

Journal of Applied Animal Research

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Three serological methods used in the diagnosis of bovine brucellosis were conducted to assess the diagnostic sensitivity of the tests in brucellosis-infected herds. A total of 487 unvaccinated bovine serum samples collected from infected herds in the southeast region of Turkey having history of abortion with a known bacteriological status for the last three years were tested for anti-Brucella antibodies detection using the Rose Bengal plate test (RBPT), the complement fixation test (CFT) and indirect enzyme-linked immunosorbent assay (i-ELISA). A protein A/G conjugate was used in i-ELISA in order to reduce background reactivity and to use the test in multispecies if required. The serum samples were found as positive in 74.3%, 83.7% and 81.3% when we employed the CFT, i-ELISA and RBPT, respectively. i-ELISA could be used as a single test or combined with the RBPT for serologic diagnosis for brucellosis in infected herds. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

“…i-ELISA has been shown to be a highly sensitive and suitable test for large-scale screening for bovine brucellosis. Different i-ELISA techniques were the results of different antigens used and conjugates chosen for the test (Sutherland et al 1986;Wright et al 1990;Jacques et al 1998;Garcia-Bocanegra et al 2014;O'Grady et al 2014). …”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of three serological tests for the detection of Brucella antibodies from infected cattle herds

Tel

Keskin

2016

Journal of Applied Animal Research

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“…When the contaminant colony counts were taken into account, the media's inhibition ability was listed by focusing on the range of the contaminant burden. We have classified the ranges into 4 groups; namely, 1 total inhibition group without any contaminant colonies and 3 partial inibition groups with less than 10, ones between 10 and 100, and ones with more than 100 colonies (26,28,29). Pearson Chi-Square Test in SPSS18.0 program was used to evaluate the results of this study.…”

Section: Methodsmentioning

confidence: 99%

The Evaluation of Brucella Spp. Isolation Rates in Ruminant Abortion Cases by Using Different Selective Media

Karagül

İkiz

2018

Macedonian Veterinary Review

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The aim of this study is to evaluate the success of Brucella spp. isolation in ruminant abortion cases by using different selective media. To this end, 58 samples from ruminant abortion cases were utilized. 4 selective media; namely, Farrell Medium (FM), CITA Medium (CM), Modified Thayer Martin (MTM) and Jones & Morgan (JM) were preferred for isolation. In addition to these, one medium with antibiotics was used to extend the range of the results. Suspensions prepared from organ and fetal stomach contents were inoculated to media plates and incubated at 37C° for 5-8 days in 5-10% CO2 condition. Conventional biotyping method was used to identify Brucella isolates within the level of species and biovar. MTM (67.2%) and Farrell (65.5%) outperformed the other media with regards to isolation rate. However, regarding the inhibition ability against contaminant microrganisms, Farrell (86.2%) and CITA (72%) have the highest and second highest percentages respectively. The media’s inhibition ability was examined in the samples in which Brucella spp. isolation occurred to be able to investigate the correlations between isolation and inhibition. Lower isolation percentage was observed in the samples in which the media displayed the lowest inhibition ability against contaminants. In this context, using two different selective media with high inhibition ability against contaminants may be recommended to enhance the isolation rate. Moreover, the components stimulating the growth of Brucella strains might be added to the media to obtain better results.

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“…However, the OIE does not recommend nowadays the use of SAT for international trade in cattle [59] due to its lower specificity compared to CFT [59]. Nevertheless, SAT (and also the FPA) has demonstrated a higher sensitivity compared with the CFT for the detection of recently infected animals [100]. In swine, SAT has also been widely used [101,102], and it is currently referred as a confirmatory technique in the EU international trade in pigs, although the OIE highlights that it may show a limited specificity due to the presence of nonspecific IgM antibodies in swine serum [61].…”

Section: Other Indirect Diagnostic Tests Based On the Humoral Immune mentioning

confidence: 99%

“…For this reason, negative culture result should not be considered as definitive evidence to rule out Brucella infection in an animal/herd [29,120]. In fact, the sensitivity of culture is highly variable, depending on the stage of infection, the specimens analyzed, and the number of samples cultured [100,102,118,[121][122][123]].…”

Section: Bacteriologymentioning

confidence: 99%

Control of Animal Brucellosis — The Most Effective Tool to Prevent Human Brucellosis

García‐Seco¹,

Domı́nguez²,

Álvarez³

2015

Updates on Brucellosis

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The World Health Organization classifies brucellosis as one of the seven neglected endemic zoonosis which contribute to the perpetuation of poverty in developing countries. Although most of the developed countries are free from this important zoonosis, brucellosis has still a widespread distribution in the Mediterranean region, the Middle East, Central Asia, and parts of Latin America, making it a global problem. Nearly half a million of new cases of human brucellosis are reported each year around the world, in which animals (or products of animal origin) are the most likely source of infection. Brucella melitensis, the main etiologic agent of small ruminant brucellosis, is the most prevalent specie involved in cases of human disease in most parts of the world. Additionally, Brucella abortus (main responsible of bovine brucellosis) and Brucella suis (the most common etiological agent of porcine brucellosis) are often associated with human brucellosis. In animal production, brucellosis has a strong economic impact due not only to its direct consequences (e.g., reproductive failures) but also to indirect loses (e.g., trade restrictions). The problem of brucellosis could be considered a clear example of the need for a "One World, One Health" strategy, given that the only approach to achieve its control and subsequent eradication is the cooperation between public and animal health authorities. The prevention of human brucellosis cannot be achieved without the control of the disease in the animals, as exemplified by the impact that the early measures adopted in the beginning of the 20th century forbidding the consumption of goat milk had on the prevalence of the disease in the British soldiers in Malta. When the prevalence of the disease in the animal population is high or when eradication cannot be achieved due to other factors (e.g., lack of economic resources), its control in livestock must be the first objective. When deciding the optimal approach to tackle the disease, the prevalence of animal brucellosis is not the only parameter to consider by the decision makers since other epidemiological and economic aspects should be considered in order to implement the most adequate control strategy in each region. Cooperation between all stakeholders involved is a cornerstone in the success of any control strategy. Strict biosafety and management measures, vaccination, and test-and-slaughter strategy are recognized as the most ef

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A comparative assessment of culture and serology in the diagnosis of brucellosis in dairy cattle

Cited by 25 publications

References 28 publications

Comparative evaluation of three serological tests for the detection of Brucella antibodies from infected cattle herds

Comparative evaluation of three serological tests for the detection of Brucella antibodies from infected cattle herds

The Evaluation of Brucella Spp. Isolation Rates in Ruminant Abortion Cases by Using Different Selective Media

Control of Animal Brucellosis — The Most Effective Tool to Prevent Human Brucellosis

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