A comparative and functional genomics analysis of the genus<i>Romboutsia</i>provides insight into adaptation to an intestinal lifestyle

Gerrritsen, Jacoline; Hornung, Bastian; Ritari, Jarmo; Paulín, Lars; Rijkers, Ger T.; Schaap, P.J.; Vos, Willem M. de; Smidt, Hauke

doi:10.1101/845511

Cited by 69 publications

(52 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the genome analysis of Romboutsia spp. showed that this genus contains a versatile array of metabolic capabilities related to carbohydrate utilization and fermentation of single amino acids (Gerritsen, 2015). Moreover, E. coprostanoligenes is documented to have the capability of removing the cholesterol (Freier et al, 1994), and Acetobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Comparing the Microbial Community in Four Stomach of Dairy Cattle, Yellow Cattle and Three Yak Herds in Qinghai-Tibetan Plateau

et al. 2019

View full text Add to dashboard Cite

Yak ( Bos grunniens ) is an unique ruminant species in the Qinghai-Tibetan Plateau (QTP). The ruminant gastrointestinal tract (GIT) microbiota is not only associated with the nutrients metabolism, but also contributes to the host’s local adaptation. Examining the microbiota between cattle and yak in different geography could improve our understanding about the role of microbiota in metabolism and adaptation. To this end, we compared the microbiota in rumen, reticulum, omasum, and abomasum of dairy cattle, yellow cattle, and three yak herds (WQ yak, SZ yak, and ZB yak) lived in different altitude, based on sequencing the bacterial 16S rRNA gene on Illumina Miseq. The bacterial diversity was significantly different among five breeds, whereas the difference among four stomach regions is limited. The phyla Bacteroidetes and Firmicutes were the dominated bacteria regardless of breeds and regions. The nonmetric multidimensional scaling (NMDS) results showed that the microbiota in dairy cattle, yellow cattle and WQ yak significantly differed from that in SZ yak and ZB yak for all four stomach compartments. Canonical correlation analysis revealed that Prevotella and Succiniclasticum spp. were abundant in dairy cattle, yellow cattle and WQ yak, whereas the Christensenellaceae R7 group and the Lachnospiraceae UCG 008 group were prevalent in SZ yak and ZB yak. Moreover, the microbiota in WQ yak was significantly different from that in SZ yak and ZB yak, which were characterized by the higher relative abundance Romboutsia spp., Eubacterium coprostanoligenes , Acetobacter spp., Mycoplasma spp., and Rikenellaceae RC9 group. Overall, these results improves our knowledge about the GIT microbiota composition of QTP ruminant.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparing the Microbial Community in Four Stomach of Dairy Cattle, Yellow Cattle and Three Yak Herds in Qinghai-Tibetan Plateau

et al. 2019

View full text Add to dashboard Cite

show abstract

“…As work on these species has not been conducted in poultry, mapping of the human intestinal microbiome demonstrates that Romboutsia spp. is present in nutrient-rich environments where exogenous sources of amino acids and vitamins are abundantly available [ 27 ]. Whilst there is limited literature on this species functionality in poultry, the carbon sources it utilizes include D-arabinose, L-fucose, D-galactose, D-glucose, raffinose, and sucrose and it can produce acetate and formate [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Multienzyme Super-Dosing in Broiler Chicken Diets: The Implications for Gut Morphology, Microbial Profile, Nutrient Digestibility, and Bone Mineralization

Madigan-Stretton

Mikkelsen

Soumeh

2020

Animals

View full text Add to dashboard Cite

Optimizing gut health has a large impact on nutrient digestibility and bioavailability, and super-dosing feed enzymes may be one solution to achieve this. A 42-day grow-out trial was conducted using 192 Ross 308 broilers to determine if super-dosing Natuzyme at 0 g/t, 350 g/t, 700 g/t, and 1000 g/t dose rates could improve the gut morphology, alter the cecal microbial profile, enhance bone mineralization, and improve nutrient digestibility of a wheat–corn–soybean diet (six replicates per treatment, eight birds per pen). One bird per pen was slaughtered at day 42 and gut morphology, cecal microbial profile, and nutrient digestibility were studied. The addition of enzymes tended to increase the villus height in the duodenum, villus height, width, and crypt depth in the jejunum, and villus width and the number of goblet cells in the ileum. Microbial profiling revealed diverse communities; however, they did not significantly differ between treatment groups. Yet, 700 g/t Natuzyme promoted microbes belonging to the genus Romboutsia and Ruminococcus gauvreauii, while 1000 g/t Natuzyme promoted Barnesiella species. The nutrient digestibility demonstrated a significant improvement in all enzyme doses compared to the control. In conclusion, based on the outcomes of this study, a dose rate of 700 g/t Natuzyme is recommended to improve gut morphology and nutrient digestibility, and promote unique microbes which aid in feed efficiency.

show abstract

“…Romboutsia (OTU5) had a high abundance in the jejunum across age compared to the colon. The genus Romboutsia associates with carbohydrate utilization, fermentation of amino acids and anaerobic respiration [43]. Clostridiales (OTU20), which had high abundances across all ages in the jejunum, is a saprophytic organism that ferments plant polysaccharides.…”

Section: Biogeography Dissimilarities Of Gut Microbiotamentioning

confidence: 99%

Longitudinal Investigation of the Gut Microbiota in Goat Kids from Birth to Postweaning

et al. 2020

View full text Add to dashboard Cite

Early microbial colonization in the gut impacts animal performance and lifelong health. However, research on gut microbial colonization and development in young ruminants, especially after weaning, is currently limited. In this study, next-generation sequencing technology was performed to investigate the temporal dynamic changes of the microbial community in the jejunum and colon of goats at 1, 7, 14, 28, 42, 56, 70, and 84 days (d) of age. As age increased, significant increases in microbial diversity, including the number of Observed OTUs and the Shannon Index, were observed in both the jejunum and colon. Regarding beta diversity, significant shifts in community membership and structure from d1 to d84 were observed based on both Bray–Curtis and Jaccard distances. With increasing age, dominant genera in the jejunum shifted from Lactobacillus to unclassified Ruminococcaceae, unclassified Lachnospiraceae and unclassified Clostridiales through starter supplementation, whereas colonic dominant genera changed from Lactobacillus and Butyricicoccus, within d1–d28, to unclassified Ruminococcaceae, unclassified Clostridiales and Campylobacter after solid diet supplementation. The linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed bacterial features that are stage-specific in the jejunum and colon, respectively. In the jejunum and colon, a significantly distinct structure and membership of the microbiota was observed across all ages. The growth stage-associated microbiota in each gut compartment was also identified as a marker for biogeography. Our data indicate the temporal and spatial differences of the gut microbiota in goats are important for their performance and health. Early microbial colonization can influence microbial composition in later life (e.g., post-weaning phase). This study provides insights that the temporal dynamics of gut microbiota development from newborn to post-weaning can aid in developing feeding strategies to improve goat health and production.

show abstract

A comparative and functional genomics analysis of the genusRomboutsiaprovides insight into adaptation to an intestinal lifestyle

Cited by 69 publications

References 111 publications

Comparing the Microbial Community in Four Stomach of Dairy Cattle, Yellow Cattle and Three Yak Herds in Qinghai-Tibetan Plateau

Comparing the Microbial Community in Four Stomach of Dairy Cattle, Yellow Cattle and Three Yak Herds in Qinghai-Tibetan Plateau

Multienzyme Super-Dosing in Broiler Chicken Diets: The Implications for Gut Morphology, Microbial Profile, Nutrient Digestibility, and Bone Mineralization

Longitudinal Investigation of the Gut Microbiota in Goat Kids from Birth to Postweaning

Contact Info

Product

Resources

About