A Comparative Analysis of Metabolic Profiles of Embryonic Skeletal Muscle from Lantang and Landrace Pigs

Cai, Shaohui; Duo, Tianqi; Wang, Xiaoyu; Tong, Xiao Yan; Luo, Cheng; Chen, Yaosheng; Li, Jianhao; Mo, Delin

doi:10.3390/ani12040420

Cited by 3 publications

(3 citation statements)

References 65 publications

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“…Metabolomic studies should be carried out to characterize cultivated meat cell lines because the requirements of different cell types vary by species, breed, and cell line. Several studies have shown that different breeds of pigs have different metabolic profiles (Cai et al., 2022), which in turn affects cellular metabolic processes like proliferative capacity (X. ‐Q.…”

Section: Studying Metabolismmentioning

confidence: 99%

“…However, this may require creating alternate genome‐scale models because different breeds of the same organism have different metabolic requirements. For example, it is possible to differentiate between Lantang and Landrace pigs using metabolomics because 40 metabolites in Lantang pigs have a higher abundance than in Landrace pigs (Cai et al., 2022), and the cells derived from these two pig breeds have different proliferative capacities (X.‐Q. Wang et al., 2012).…”

Section: Gaps In Knowledge and Future Directionsmentioning

confidence: 99%

“…Interestingly, it has been shown that different breeds of pigs have different metabolic profiles. Cai et al found that Lantang pigs have a higher abundance of 40 metabolites as compared to Landrace pigs (Cai et al, 2022). This difference seems to be related to cellular metabolic processes, as cells derived from Lantang pigs have higher proliferative capacity than cells derived from Landrace pigs (X.…”

Section: Pigs (Sus Scrofa Domesticus)mentioning

confidence: 99%

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Systems biology and metabolic modeling for cultivated meat: A promising approach for cell culture media optimization and cost reduction

Gomez Romero,

Boyle

2023

Comp Rev Food Sci Food Safe

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The cultivated meat industry, also known as cell-based meat, cultured meat, lab-grown meat, or meat alternatives, is a growing field that aims to generate animal tissues ex-vivo in a cost-effective manner that achieves price parity with traditional agricultural products. However, cell culture media costs account for 55%-90% of production costs. To address this issue, efforts are aimed at optimizing media composition. Systems biology-driven approaches have been successfully used to improve the biomass and productivity of multiple bioproduction platforms, like Chinese hamster ovary cells, by accelerating the development of cell line-specific media and reducing research and development and production costs related to cell media and its optimization. In this review, we summarize systems biology modeling approaches, methods for cell culture media and bioprocess optimization, and metabolic studies done in animals of interest to the cultivated meat industry. More importantly, we identify current gaps in knowledge that prevent the identification of metabolic bottlenecks. These include the lack of genome-scale metabolic models for some species (pigs and ducks), a lack of accurate biomass composition studies for different growth conditions, and 13 C-metabolic flux analysis (MFA) studies for many of the species of interest for the cultivated meat industry (only shrimp and duck cells have been subjected to 13 C-MFA). We also highlight the importance of characterizing the metabolic requirements of cells at the organism, breed, and cell line-specific levels, and we outline future steps that this nascent field needs to take to achieve price parity and production efficiency similar to those of other bioproduction platforms. Practical Application: Our article summarizes systems biology techniques for cell culture media design and bioprocess optimization, which may be used to significantly reduce cell-based meat production costs. We also present the results of experimental studies done on some of the species of interest to the cultivated meat industry and highlight why modeling approaches are required for multiple species, cell-types, and cell lines.

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Section: Studying Metabolismmentioning

confidence: 99%

Section: Gaps In Knowledge and Future Directionsmentioning

confidence: 99%

Section: Pigs (Sus Scrofa Domesticus)mentioning

confidence: 99%

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Systems biology and metabolic modeling for cultivated meat: A promising approach for cell culture media optimization and cost reduction

Gomez Romero,

Boyle

2023

Comp Rev Food Sci Food Safe

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Widely Targeted Metabolomic Analysis Revealed the Diversity in Milk from Goats, Sheep, Cows, and Buffaloes and Its Association with Flavor Profiles

Zhang,

Wang,

Liu

et al. 2024

Foods

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The milk flavor can be attributed to the presence of numerous flavor molecules and precursors. In this study, we employed widely targeted metabolomic analysis techniques to analyze the metabolic profiles of various milk samples obtained from goats, sheep, dairy cows, and buffaloes. A total of 631 metabolites were identified in the milk samples, which were further categorized into 16 distinct classes. Principal component analysis (PCA) suggested that the metabolite profiles of samples from the same species exhibit clustering, while separated patterns of metabolite profiles are observed across goat, sheep, cow, and buffalo species. The differential metabolites between the groups of each species were screened based on fold change and variable importance in projection (VIP) values. Five core differential metabolites were subsequently identified, including 3-(3-hydroxyphenyl)-3-hydroxypropanoic acid, inosine 5′-triphosphate, methylcysteine, N-cinnamylglycine, and small peptide (L-tyrosine–L-aspartate). Through multiple comparisons, we also screened biomarkers of each type of milk. Our metabolomic data showed significant inter-species differences in the composition and concentration of some compounds, such as organic acids, amino acids, sugars, nucleotides, and their derivatives, which may affect the overall flavor properties of the milk sample. These findings provided insights into the molecular basis underlying inter-species variations in milk flavor.

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Amyotrophic Lateral Sclerosis Pathoetiology and Pathophysiology: Roles of Astrocytes, Gut Microbiome, and Muscle Interactions via the Mitochondrial Melatonergic Pathway, with Disruption by Glyphosate-Based Herbicides

Anderson¹

2022

IJMS

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The pathoetiology and pathophysiology of motor neuron loss in amyotrophic lateral sclerosis (ALS) are still to be determined, with only a small percentage of ALS patients having a known genetic risk factor. The article looks to integrate wider bodies of data on the biological underpinnings of ALS, highlighting the integrative role of alterations in the mitochondrial melatonergic pathways and systemic factors regulating this pathway across a number of crucial hubs in ALS pathophysiology, namely glia, gut, and the muscle/neuromuscular junction. It is proposed that suppression of the mitochondrial melatonergic pathway underpins changes in muscle brain-derived neurotrophic factor, and its melatonergic pathway mimic, N-acetylserotonin, leading to a lack of metabolic trophic support at the neuromuscular junction. The attenuation of the melatonergic pathway in astrocytes prevents activation of toll-like receptor agonists-induced pro-inflammatory transcription factors, NF-kB, and yin yang 1, from having a built-in limitation on inflammatory induction that arises from their synchronized induction of melatonin release. Such maintained astrocyte activation, coupled with heightened microglia reactivity, is an important driver of motor neuron susceptibility in ALS. Two important systemic factors, gut dysbiosis/permeability and pineal melatonin mediate many of their beneficial effects via their capacity to upregulate the mitochondrial melatonergic pathway in central and systemic cells. The mitochondrial melatonergic pathway may be seen as a core aspect of cellular function, with its suppression increasing reactive oxygen species (ROS), leading to ROS-induced microRNAs, thereby altering the patterning of genes induced. It is proposed that the increased occupational risk of ALS in farmers, gardeners, and sportsmen and women is intimately linked to exposure, whilst being physically active, to the widely used glyphosate-based herbicides. This has numerous research and treatment implications.

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A Comparative Analysis of Metabolic Profiles of Embryonic Skeletal Muscle from Lantang and Landrace Pigs

Cited by 3 publications

References 65 publications

Systems biology and metabolic modeling for cultivated meat: A promising approach for cell culture media optimization and cost reduction

Systems biology and metabolic modeling for cultivated meat: A promising approach for cell culture media optimization and cost reduction

Widely Targeted Metabolomic Analysis Revealed the Diversity in Milk from Goats, Sheep, Cows, and Buffaloes and Its Association with Flavor Profiles

Amyotrophic Lateral Sclerosis Pathoetiology and Pathophysiology: Roles of Astrocytes, Gut Microbiome, and Muscle Interactions via the Mitochondrial Melatonergic Pathway, with Disruption by Glyphosate-Based Herbicides

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