A Comparative Analysis of Invasive and Non-Invasive Method of Bone Marrow Stromal Cell Isolation

Abstract

The conventional method of Bone Marrow Stromal Cell (BMSC) isolation from live subjects are complex due to involvement of lot of expert personnel and pre and post operational medication and cares. Moreover, the concerning ethical issues also pose lots of restrictions for isolation of BMSC's making stem cells research restricted to certain elite laboratories only. This study aims to compare between the regular aspiration (invasive) method and an alternative, straight forward and non-invasive method of BMSC harvest. The BMSCs were harvested by both invasive and non-invasive methods and cultured in MSC (Mesenchymal Stem Cell) medium. The cells were undergone visual assessment of growth dynamics as well as identification and characterization of MSC cells, based on microscopic examination. Both of these tested methods successfully yielded significant amount of BMSC that were found to be identical in morphology, growth dynamics and in vitro cultural properties. Unlike the invasive method that requires a live animal, the non-invasive method relies on post-slaughtered bone and therefore obviates the requirement of skill personnel and setup. Eventually, the used bone from already dead animal no longer becomes the issue of conflict with animal ethics and welfare. The ease in cell harvest and lack of ethical barrier would definitely make BMSC harvest convenient and therefore, anticipated to be well accepted in resource poor laboratories around the globe.

Adipogenic differentiation of culture-expanded bone marrow derived porcine mesenchymal stem cells

“…Therefore, we have isolated mesenchymal stem cells from bone marrow (Fig. 1A), as per protocols published previously (Santra et al 2015(Santra et al , 2017. These cells were propagated in MSC culture medium.…”

“…For the present study, porcine mesenchymal stem cells previously isolated and characterized earlier (Santra et al 2017(Santra et al , 2015 were used.…”

“…Present study was conceived to establish a porcine mesenchymal stem cell line for adipogenesis in culture. Earlier, we had demonstrated the harvest of bone marrow derived MSCs following either invasive method from live animal or from long bones of dead/slaughtered animal (Santra et al 2015(Santra et al , 2017. In the current study, we demonstrated these isolated stromal MSCs are suitable for induced adipogenic differentiation.…”