A comparative analysis of different methods for routine blood component preparation

Platelet Activation in Stored Platelet Concentrates: Comparision of Two Methods Preparation

“…The present paper describes an in vitro comparison of both preparations. The studies suggested that the degree of in vitro activation as evidenced by the expression of CD62P and Annexin V binding were dependent on the different preparative methods [4][5][6][7][8][9][10][11][12][13][14][15][16]17,18]. We found that the extent of activation was significantly higher in PRP-PCs than in BCs.…”

“…Heaton et al [4] compared components prepared by the PRP-PC and BC methods. The results of [5] were also similar and reported WBC contamination/unit in PRP-PC and BC.…”

“…Preparation conditions and storage of platelet for transfusion may cause platelet activation, which contributes to decreased ability of stored platelet to function and to survive in vivo after transfusion compared with that seen with freshly prepared platelets [1][2][3][4]. Platelet transfusion therapy has played an important role in the management of patients [2,4].…”

“…We here report on the difference between both platelet concentrates in functional properties of platelet in BC and PRP-PC are compared over storage of 5 days. Processing and storage may affect both platelet morphology and function and they may thus be less effective when transfused than fresh platelets [1,[3][4][8][9]. There are several methods for quality control of platelet components including cell counting, pH, swirling and markers for platelet activation [8,[10][11].…”

“…Platelet transfusion therapy has played an important role in the management of patients [2,4]. Today, platelet concentrates are routinely manufactured from whole blood by differential centrifugation buffy coat-derived platelet concentrates (BC) or by platelet rich plasma-platelet concentrates (PRP-PC) and plateletpheresis [5][6][7].…”