A Comparative Analysis of Aquatic and Polyethylene-Associated Antibiotic-Resistant Microbiota in the Mediterranean Sea

Sucato, Arianna; Vecchioni, Luca; Savoca, Dario; Presentato, Alessandro; Arculeo, Marco; Alduina, Rosa

doi:10.3390/biology10030200

Cited by 21 publications

(12 citation statements)

References 68 publications

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“…All tet genes detected in this study (tet (A), tet (B), tet (C), tet (D), tet (E), tet (G)) are implicated in the mechanism of tetracycline extrusion from the bacterial cell, that represents a common strategy of tetracycline resistance. We did not detect tet (O) and tet (W), that were recently found to be diffused in Gram-negative bacteria isolated from water samples of various origin [34]. The most frequently detected gene was tet (A), harbored by 14/25 (56%) strains; this gene encodes a subunit of the tetracycline efflux pump found in many species of Gram-negative bacteria [35].…”

Section: Discussionmentioning

confidence: 61%

Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in Salmonella spp. Strains Isolated from Animals and Food

et al. 2021

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Salmonella spp. is among the leading causes of foodborne infections in humans and a large number of animals. Salmonella spp. is a pathogen involved in the dissemination of antimicrobial resistance because it can accumulate antibiotic resistance genes (ARGs). In this study, the antibiotic resistance profile to 15 antibiotics, belonging to six different classes, of 60 strains of Salmonella spp. collected from pets, farm animals, wildlife, and food in Sicily (Italy) was investigated by the Kirby-Bauer method. Given that almost 33.3% of the Salmonella spp. strains were resistant to tetracycline, Real-Time PCR analysis was applied on all the 60 strains to detect the presence of eight selected tet resistance genes. Besides, the presence of the int1 gene, related to the horizontal gene transfer among bacteria, was also investigated in all the strains by Real-Time PCR analysis. Our data showed that 56% of the isolated strains harbored one or more tet resistance genes and that these strains were most frequently isolated from animals living in close contact with humans. Concerning int1, 17 strains (28.3%) harbored this genetic element and eight of these simultaneously contained tet genes. The results of this study highlight the importance of using a molecular approach to detect resistance genetic determinants, whose spread can increase the diffusion of multidrug-resistant strains. Besides, the study of zoonotic bacteria such as Salmonella spp. which significantly contribute to ARGs dissemination should always follow a One Health approach that considers the health of humans, animals, and the environment to be closely related.

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Section: Discussionmentioning

confidence: 61%

Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in Salmonella spp. Strains Isolated from Animals and Food

et al. 2021

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“…We could surmise that the high abundance of Proteobacteria in the ES could depend on maternal influence and environmental components (such as the mineralogical substrate of the beach, seawater, and exogenous bacterial source). Conversely, Firmicutes and Bacteroidetes are less abundant in the sand and seawater [ 64 ], thus suggesting an influence of the mother in providing eggs with these bacteria during the passage through the oviduct or cloaca.…”

Section: Discussionmentioning

confidence: 99%

Unveiling the egg microbiota of the loggerhead sea turtle Caretta caretta in nesting beaches of the Mediterranean Sea

et al. 2022

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Microbes have central roles in the development and health of animals, being the introduction of specific microbial species a potential conservation strategy to protect animals from emerging diseases. Thus, insight into the microbiota of the species and their habitats is essential. In this manuscript, we report for the first time the bacterial composition of all the components (eggshells of hatched and unhatched eggs, internal content of unhatched eggs, intestinal content of hatchling and pipping sea turtles, and sand) of three nesting beaches of Caretta caretta along the Italian coasts of the Mediterranean Sea. The analysis of 26 amplicon samples was carried out using next-generation sequencing analysis, targeting V3–V4 regions of the bacterial 16S rRNA gene. Samples featured mainly Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes, whose percentages depended on the sample type. Our results showed that, although from different sampling sites, the internal content of the unhatched eggs, intestinal content of hatchling and pipping sea turtles share the microbiota, which was yet different from that of eggshells and sand of the same nesting beach. This study suggests the maternal and environmental influence alongside a protective role of eggshells in shaping the egg microbiota of Caretta caretta sea turtles.

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“…In the last ten years, the worldwide production of plastic and plastic-based materials increased up to 20-fold, generating large amounts of waste that excessively accumulate in the environment (Gouveia, Biernacki, Castro, Goncalves, & Souza, 2019;Shivangi, Dorairaj, Negi, Shetty, & 2021). This event poses crucial ecological and toxicological problems, as several synthetic polymers used for producing plastic-based materials can cause severe health issues and can be carrier of microorganisms (Shivangi et al, 2021;Gao, He, Sun, He, & Zeng 2019;Sucato et al, 2021). Thus, we are witnessing an increased demand for safer alternatives that can replace non-biodegradable plastic-based materials.…”

Section: Introductionmentioning

confidence: 99%

Cross-linked natural IntegroPectin films from Citrus biowaste with intrinsic antimicrobial activity

Piacenza¹,

Presentato²,

Alduina³

et al. 2022

Preprint

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Pectin recovered via hydrodynamic cavitation (IntegroPectin) from lemon and grapefruit agri-food waste intrinsically containing antimicrobial bioactive substances (flavonoids, phenolic acids, terpenes, and terpenoids) was used to generate innovative and eco-compatible films that efficiently inhibit the growth of Gram-negative pathogens. Extensive characterization of films confirmed the presence of these substances, which differently interact with the polysaccharide polymer (pectin), plasticizer (glycerol), surfactant (Tween 60), and cross-linker (Ca2+), conferring to these films a unique structure. Besides, IntegroPectin-based films constitute sustained, controlled, and slow-release (up to 72h) systems for bioactive substances in an aqueous environment. This feature is crucial for the good in vitro antimicrobial activity exerted by IntegroPectin films against three Gram-negative bacteria (two indicator pathogen strains Pseudomonas aeruginosa ATCC 10145, P. aeruginosa PAO1, and the clinical isolate Klebsiella pneumoniae) that are involved in the global emergence of the antimicrobial resistance.

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A Comparative Analysis of Aquatic and Polyethylene-Associated Antibiotic-Resistant Microbiota in the Mediterranean Sea

Cited by 21 publications

References 68 publications

Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in Salmonella spp. Strains Isolated from Animals and Food

Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in Salmonella spp. Strains Isolated from Animals and Food

Unveiling the egg microbiota of the loggerhead sea turtle Caretta caretta in nesting beaches of the Mediterranean Sea

Cross-linked natural IntegroPectin films from Citrus biowaste with intrinsic antimicrobial activity

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