A compact pulse-modulation air plasma jet for the inactivation of chronic wound bacteria: Bactericidal effects &amp; host safety

Thana, Phuthidhorn; Kuensaen, Chakkrapong; Poramapijitwat, Pipath; Sarapiroma, Sureeporn; Yu, L.D.; Boonyawan, Dheerawan

doi:10.1016/j.surfcoat.2020.126229

Cited by 15 publications

(4 citation statements)

References 46 publications

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“…The previous report related to plasma generation was based on argon or helium gas as a carrier gas to generate plasma; however, our study provided safety information of plasma devices which use air as a carrier gas. Previous studies by Thana in 2019 and 2020 [ 20 , 21 ] demonstrated the generation of reactive oxygen and nitrogen species (RONS) such as nitric oxide, hydroxyl radical, atomic oxygen, and ozone using a compact low-temperature plasma jet device similar to a CAPJ, with a generated plasma temperature of approximately 35 °C. These studies also observed antibacterial activity and noncytotoxicity to dermal fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

A Nonclinical Safety Evaluation of Cold Atmospheric Plasma for Medical Applications: The Role of Genotoxicity and Mutagenicity Studies

Yarangsee,

Khacha-ananda,

Pitchakarn

et al. 2024

Life

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Atmospheric nonthermal plasma (ANTP) has rapidly evolved as an innovative tool in biomedicine with various applications, especially in treating skin diseases. In particular, the formation of reactive oxygen species (ROS) and nitrogen species (RNS), which are generated by ANTP, plays an important role in the biological signaling pathways of human cells. Unfortunately, excessive amounts of these reactive species significantly result in cellular damage and cell death induction. To ensure the safe application of ANTP, preclinical in vitro studies must be conducted before proceeding to in vivo or clinical trials involving humans. Our study aimed to investigate adverse effects on genetic substances in murine fibroblast cells exposed to ANTP. Cell viability and proliferation were markedly reduced after exposing the cells with plasma. Both extracellular and intracellular reactive species, especially RNS, were significantly increased upon plasma exposure in the culture medium and the cells. Notably, significant DNA damage in the cells was observed in the cells exposed to plasma. However, plasma was not classified as a mutagen in the Ames test. This suggested that plasma led to the generation of both extracellular and intracellular reactive species, particularly nitrogen species, which affect cell proliferation and are also known to induce genetic damage in fibroblast cells. These results highlight the genotoxic and mutagenic effects of ANTP, emphasizing the need for the cautious selection of plasma intensity in specific applications to avoid adverse side effects resulting from reactive species production.

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Section: Introductionmentioning

confidence: 99%

A Nonclinical Safety Evaluation of Cold Atmospheric Plasma for Medical Applications: The Role of Genotoxicity and Mutagenicity Studies

Yarangsee,

Khacha-ananda,

Pitchakarn

et al. 2024

Life

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“…This makes it a promising technology for a number of applications that competes against low-pressure plasma. Among the applications, surface functionalization [1][2][3][4], film growth [5,6], medical application [7][8][9][10][11] and water purification [12,13]. So far, many sources at atmospheric pressure generating non-equilibrium plasma have been developed as localized and extended plasma among them the most common are corona discharge and dielectric barrier discharge (DBD).…”

Section: Introductionmentioning

confidence: 99%

Surface modification of polyethylene using argon and argon/methane DBD atmospheric pressure plasma jet

Bedrouni,

Ouchabane,

Almabouada

et al. 2023

Phys. Scr.

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This article investigates the surface modification of polyethylene (PE) using an atmospheric pressure plasma jet of the DBD type (APPJ) maintained with pure argon (Ar) and with a premixed gas containing 90% argon and 10 % of CH4. First and for both plasma jets, the jet length and the optical emissions coming from the plasma species were studied in terms of flow rate and applied voltage. Comparing to Ar, it has been shown that when the premixed gas is used, the jet length gets smaller, the transition point from laminar to turbulent regime was shifted to a low flow rate, and the emission of CH, H2 and C2 appeared, while O and N2 emission coming from ambient air was quenching. The PE surface treatment in both laminar and turbulent regime has been evaluated using water contact angle (WCA) measurement and attenuated total reflection (ATR). The obtained results showed that the hydrophilicity of PE has been improved in both plasma jets due to oxygen group (C=O) incorporation, but Ar plasma jet was observed to induce surface degradation compared to Ar/CH4 plasma due to the higher excited species.

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“…The influence of cold plasma depends on the action of the generation of chemically reactive species including reactive oxygen and nitrogen species like OH, O, O 3 , N 2 , charged particles, and UV radiation 17 – 19 . The interaction between the produced active species in plasma with surrounding moisture and H 2 O molecule leads to the production of H 2 O 2 , NO 2 − , and NO 3 − in liquid phase of the targeted surface 20 . Some of them possess high reduction potential that can eventually lead to the cell death in micro-organisms.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of cold atmospheric-pressure argon plasma combined with chicory (Cichorium intybus L.) extract against P. aeruginosa and E. coli biofilms

Shabani

Dezhpour

Jafari

et al. 2023

Sci Rep

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The present study reports a significant combined antibacterial activity of Cichorium intybus L. (known as Chicory) natural extract with cold atmospheric-pressure argon plasma treatment against multi-drug resistant (MDR) Gram-negative bacteria. To detect reactive species that are generated in the argon plasma, optical emission spectra were recorded. The molecular bands were allocated to the hydroxyl radicals (OH) and neutral nitrogen molecules (N2). Moreover, the atomic lines form the emitted spectra were determined to argon atoms (Ar) and the oxygen atoms (O), respectively. The results revealed that Chicory extract treatment at a concentration of 0.043 g/ml reduced the metabolic activity of P. aeruginosa cells by 42%, while, a reduced metabolic activity of 50.6% was found for E. coli biofilms. Moreover, the combination of Chicory extract with 3 min Ar-plasma introduced a synergistic effect, so that it exhibited a significantly reduced metabolic activity of P. aeruginosa to 84.1%, and E. coli ones to 86.7%, respectively. The relationship between cell viability and membrane integrity of P. aeruginosa and E. coli biofilms treated with Chicory extract and argon plasma jet were also analyzed by CLSM. It was found that after the combined treatment, a noticeable membrane disruption was formed. Besides, it was concluded that E. coli biofilms showed a higher sensitivity to Ar-plasma than P. aeruginosa biofilm at longer plasma exposure times. This study suggests that the anti-biofilm therapy based on a combined effect of Chicory extract and cold argon plasma treatment can serve as a considerable green method for treatment of antimicrobial MDR bacteria.

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A compact pulse-modulation air plasma jet for the inactivation of chronic wound bacteria: Bactericidal effects & host safety

Cited by 15 publications

References 46 publications

A Nonclinical Safety Evaluation of Cold Atmospheric Plasma for Medical Applications: The Role of Genotoxicity and Mutagenicity Studies

A Nonclinical Safety Evaluation of Cold Atmospheric Plasma for Medical Applications: The Role of Genotoxicity and Mutagenicity Studies

Surface modification of polyethylene using argon and argon/methane DBD atmospheric pressure plasma jet

Antimicrobial activity of cold atmospheric-pressure argon plasma combined with chicory (Cichorium intybus L.) extract against P. aeruginosa and E. coli biofilms

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