A compact planar positron imaging system

Uchida, Hiroshi; Okamoto, Takashi; Ohmura, T.; Shimizu, Katsuji; Satoh, N.; Koike, Tatsuki; Yamashita, T.

doi:10.1016/j.nima.2003.08.165

Cited by 63 publications

(56 citation statements)

References 25 publications

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“…Mice with an acrylic plate were placed at the center position between two planar positron whole-body imaging system (PPIS) detectors and scans with 10-min time frame were performed for 10 h with 60 3 10-min frame using a planar positron imaging system (PPIS-4800; Hamamatsu Photonics, Hamamatsu, Japan) (21). PPIS data were analyzed by using ImageJ software and expressed as counts per pixel.…”

Section: Animalsmentioning

confidence: 99%

In Vivo Molecular Imaging Analysis of a Nasal Vaccine That Induces Protective Immunity against Botulism in Nonhuman Primates

Yuki

Nochi

Harada

et al. 2010

The Journal of Immunology

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Section: Animalsmentioning

confidence: 99%

In Vivo Molecular Imaging Analysis of a Nasal Vaccine That Induces Protective Immunity against Botulism in Nonhuman Primates

Yuki

Nochi

Harada

et al. 2010

The Journal of Immunology

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“…animation) of the two-dimensional distribution of a radiotracer within a living organism without contact. Its principle is the same as that of positron emission tomography, which has been widely used for medical diagnosis, but PETIS was specially designed for studying plants and has been applied to many studies on plant nutrition over the last decade (Uchida et al, 2003;Fujimaki, 2007;Fujimaki et al, 2010). Recently, the transport of metals, including iron (Ishimaru et al, , 2007Tsukamoto et al, 2009), zinc , and manganese , in intact graminaceous plants has been visualized using PETIS.…”

mentioning

confidence: 99%

Tracing Cadmium from Culture to Spikelet: Noninvasive Imaging and Quantitative Characterization of Absorption, Transport, and Accumulation of Cadmium in an Intact Rice Plant

et al. 2010

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We characterized the absorption and short-term translocation of cadmium (Cd) in rice (Oryza sativa 'Nipponbare') quantitatively using serial images observed with a positron-emitting tracer imaging system. We fed a positron-emitting 107 Cd (halflife of 6.5 h) tracer to the hydroponic culture solution and noninvasively obtained serial images of Cd distribution in intact rice plants at the vegetative stage and at the grain-filling stage every 4 min for 36 h. The rates of absorption of Cd by the root were proportional to Cd concentrations in the culture solution within the tested range of 0.05 to 100 nM. It was estimated that the radial transport from the culture to the xylem in the root tissue was completed in less than 10 min. Cd moved up through the shoot organs with velocities of a few centimeters per hour at both stages, which was obviously slower than the bulk flow in the xylem. Finally, Cd arrived at the panicles 7 h after feeding and accumulated there constantly, although no Cd was observed in the leaf blades within the initial 36 h. The nodes exhibited the most intensive Cd accumulation in the shoot at both stages, and Cd transport from the basal nodes to crown root tips was observed at the vegetative stage. We conclude that the nodes are the central organ where xylem-to-phloem transfer takes place and play a pivotal role in the half-day travel of Cd from the soil to the grains at the grain-filling stage.

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“…Recently, a positron-emitting tracer imaging system (PETIS) that observes annihilation γ-rays produced from positron-emitting radionuclides was developed (Uchida et al, 2004). PETIS can obtain two-dimensional serial images of the distribution of tracers in real time with a wide view-range and high resolution.…”

mentioning

confidence: 99%

“…The PETIS scanner has two opposing planar detectors consisting of Bi 4 Ge 3 O 12 scintillator arrays that detect γ-rays (Nagai et al, 1996;Uchida et al, 2004). When each head of the detector detects an annihilation γ-ray at the same moment (within 20 ns), the emission point is determined as the point mid-way between the two detected points.…”

mentioning

confidence: 99%

Real-time Analysis of Photoassimilate Translocation in Intact Eggplant Fruit using 11CO2 and a Positron-emitting Tracer Imaging System

Kikuchi

Ishii

Fujimaki

et al. 2008

J. Japan. Soc. Hort. Sci.

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A recently developed positron-emitting tracer imaging system (PETIS) noninvasively produces quantitative realtime images of the movement of various compounds in plants. To clarify the mechanism of the growth and development of the fruit of eggplant (Solanum melongena L.), we fed 11 CO 2 to a leaf and monitored the translocation of 11 C-labeled photoassimilate into the fruit by PETIS. Continuous images of the translocation of [11 C]photoassimilate from the leaf to the fruit and inside the fruit were produced, with a shorter time resolution than reported previously. 11 C signal intensity in the fruit increased gradually, and its distribution was not uniform. The fruits were sliced after PETIS measurement and exposed to the imaging plates of a bio-imaging analyzer system. The resultant images showed the localization of 11 C activity inside the fruit, and indicated that translocation of photoassimilate within the fruit was not uniform. The velocity of photoassimilate translocation in the peduncle and changes in the rate of translocation of photoassimilate in the fruit were estimated by analysis of PETIS data. The velocity of photoassimilate translocation through the peduncle was estimated as 1.17 cm·min −1. About 60 min after the start of 11 CO 2 feeding, the 11 C activity of the fruit began to increase, and by 120 min it had reached about 8% of feeding 11 CO 2 . These results indicate that it took about 60 min for the first [11 C]photoassimilates to reach the fruit. Real time observation of photoassimilate translocation within a fruit has never been reported. PETIS may be a powerful tool for revealing the mechanisms of fruit development and maturity.

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A compact planar positron imaging system

Cited by 63 publications

References 25 publications

In Vivo Molecular Imaging Analysis of a Nasal Vaccine That Induces Protective Immunity against Botulism in Nonhuman Primates

In Vivo Molecular Imaging Analysis of a Nasal Vaccine That Induces Protective Immunity against Botulism in Nonhuman Primates

Tracing Cadmium from Culture to Spikelet: Noninvasive Imaging and Quantitative Characterization of Absorption, Transport, and Accumulation of Cadmium in an Intact Rice Plant

Real-time Analysis of Photoassimilate Translocation in Intact Eggplant Fruit using 11CO2 and a Positron-emitting Tracer Imaging System

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