A Compact Manually Actuated Micromanipulator

Chan, Bin-Da; Mateen, Farrukh; Chang, Chun‐Li; İçöz, Kutay; Savran, Cagri A.

doi:10.1109/jmems.2011.2174431

Cited by 13 publications

(10 citation statements)

References 13 publications

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“…To further investigate the physical composition of the two types, we weighed the biomass of individual spheres by cantilever based resonator to identify the effective density of spheres (Fig. C) . Inflamed spheres showed an average density of 1.29 g/cm 3 , which was more similar to the density of protein (1.22–1.43 g/cm 3 ) than to the density of mammalian cells (1.04–1.1 g/cm 3 ) .…”

Section: Resultsmentioning

confidence: 99%

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Characterization of autoimmune inflammation induced prostate stem cell expansion

Wang

Jerde

et al. 2015

Prostate

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Background The presence of inflammation in prostate cancer (PCa) and benign prostate hyperplasia (BPH) has been well described but the cellular mechanisms by which inflammation modulates the prostate are currently unclear. Prostate stem cells (PSC) not only maintain prostate homeostasis but also are considered to be the cell of origin of PCa and an important contributor to BPH. However, the impact of inflammation on PSC is not well understood. Therefore, we initiated studies to evaluate the effect of inflammation on PSC. Method Ovalbumin specific CD8+ T cells were intravenously delivered to intact and castrated prostate ovalbumin expressing transgenic-3 (POET-3) mice to induce inflammation. Lin (CD45/CD31)−Sca1+CD49f+ cells (LSC) and progenitor cells within LSC were determined by flow cytometry. Sorted LSC were subjected to a prostate sphere forming assay to evaluate PSC clonal propagation, proliferation, immediate differentiation, and self-renewal ability. Density of individual spheres was measured by a cantilever-based resonator weighing system. Morphology and characterization of prostate spheres was determined by hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC). Finally, immediate PSC differentiation in sphere formation was determined by immunofluorescence for epithelial cytokeratin markers cytokeratin (CK) 5 and CK8. Result Data presented here demonstrate a significant expansion of the proliferative (BrdU+) LSC population, including CK5+, p63+, CK18+ cells, as well as intermediate cells (CK5+/CK8+) in inflamed prostates. Histological images reveal that PSC from inflamed prostates produce significantly larger spheres, indicating that the enhanced proliferation observed in LSC is sustained in vitro in the absence of inflammatory mediators. In addition, cultures from inflamed PSC yielded increased number of tubule-like spheres. These tube-like spheres grown from PSCs isolated from inflamed mice exhibited stratification of a CK8+ luminal-like layer and a CK5+ basal-like layer. Notably, the numbers of spheres formed by inflamed and non-inflamed PSC were equal, suggesting that even though proliferation is enhanced by inflammation, the homeostatic level of PSC is maintained. Conclusion Induction of inflammation promotes PSC expansion and immediate differentiation through highly proliferative progenitor cells while the homeostasis of PSC is maintained.

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Section: Resultsmentioning

confidence: 99%

“…Paraformaldehyde‐fixed spheres were first air‐dried on a glass slide. Individual spheres were picked up by a micromanipulator under a microscope and placed on a cantilever‐based resonator for weighing. The experimental configurations and measurement principles pertaining to mass detection were listed in a previous study .…”

Section: Methodsmentioning

confidence: 99%

Characterization of autoimmune inflammation induced prostate stem cell expansion

Wang

Jerde

et al. 2015

Prostate

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“…1a). 38 Once the target microparticle is located on the tip of the cantilever, the resulting change in the resonance frequency of the loaded cantilever is observed. This change in the resonance frequency reveals the mass of the loaded entity.…”

Section: Introductionmentioning

confidence: 99%

On-demand weighing of single dry biological particles over a 5-order-of-magnitude dynamic range

et al. 2014

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We report a simple and highly versatile system to select and weigh individual dry biological particles. The system is composed of a microtweezer to pick and place individual particles and a cantilever-based resonator to weigh them. The system can weigh entities that vary from a red blood cell (~10(-11) g) to the eye-brain complex of an insect (~10(-6) g), covering a 5-order-of-magnitude mass range. Due to its versatility and ease of use, this weighing method is highly compatible with established laboratory practices. The system can provide complementary mass information for a wide variety of individual particles imaged using scanning electron microscopy and determine comparative weights of individual biological entities that are attached to microparticles as well as weigh fractions of individual biological entities that have been subjected to focused ion beam milling.

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“…The operation mechanism of the micro-tweezer is reported elsewhere. 24,25 The stem cell is fixed in a 2.0% Gultaraldehyde in 0.1 M Cacodylate buffer pH 7.4 solution prior to transferring. Fig.…”

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confidence: 99%

Flexible complementary metal oxide semiconductor microelectrode arrays with applications in single cell characterization

Pajouhi

Jou

Jain

et al. 2015

Applied Physics Letters

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A highly flexible microelectrode array with an embedded complementary metal oxide semiconductor (CMOS) instrumentation amplifier suitable for sensing surfaces of biological entities is developed. The array is based on ultrathin CMOS islands that are thermally isolated from each other and are interconnected by meandered nano-scale wires that can adapt to cellular surfaces with micro-scale curvatures. CMOS temperature sensors are placed in the islands and are optimally biased to have high temperature sensitivity. While no live cell thermometry is conducted, a measured temperature sensitivity of 0.15 C in the temperature range of 35 to 40 C is achieved by utilizing a low noise CMOS lock-in amplifier implemented in the same technology. The monolithic nature of CMOS sensors and amplifier circuits and their versatile flexible interconnecting wires overcome the sensitivity and yield limitations of microelectrode arrays fabricated in competing technologies.

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A Compact Manually Actuated Micromanipulator

Cited by 13 publications

References 13 publications

Characterization of autoimmune inflammation induced prostate stem cell expansion

Characterization of autoimmune inflammation induced prostate stem cell expansion

On-demand weighing of single dry biological particles over a 5-order-of-magnitude dynamic range

Flexible complementary metal oxide semiconductor microelectrode arrays with applications in single cell characterization

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