A Community-Curated DokuWiki Resource on Diagnostics, Diversity, Pathogenicity, and Genetic Control of Xanthomonads

Costa, Joana; Pothier, Joël F.; Bosis, Eran; Boch, Jens; Kölliker, Roland; Koebnik, Ralf

doi:10.1094/mpmi-11-23-0184-fi

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…Type III Secreted Effector Profile Analysis A T3S effector database was curated using amino acid sequences of 66 Xanthomonas effectors 101 (Extended Data Table 4). When available, functional annotations were retrieved from NCBI and Pfam databases 102 .…”

Section: Core Gene Phylogenymentioning

confidence: 99%

Intercontinental dissemination and diversification ofXanthomonas perforansin tomato production

Timilsina,

Iruegas-Bocardo,

Jibrin

et al. 2024

Preprint

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Tomato is a high value crop that requires quality seed each season. Tomato production often relies on international breeding and production chains with a global network to deliver seeds to growers. Xanthomonas perforans, one of the causal agents of bacterial spot of tomato, is seedborne and has rapidly emerged and displaced other bacterial spot xanthomonads in tomato production regions around the world. The objectives of this work were to determine if different tomato production regions contain genetically distinct X. perforans populations, examine genetic relatedness of strains collected in tomato seed production areas in East Asia and other production regions, estimate the timing of X. perforans population expansion relative to its first report in 1991, and evaluate variation in type III effectors, which are critical pathogenicity and virulence factors. Our results showed extensive genetic diversity in the pathogen and highly similar strains in distant tomato production regions, which is consistent with global dissemination. Furthermore, we found genetically similar strains in tomato seed production and fruit production regions, as would be expected if the pathogen was being moved through tomato production chains from a common origin. We found waves of diversification of populations in the United States, where our sampling was more extensive, before and since its first detection, consistent with emergence of new X. perforans genotypes from diverse source populations. Variation in gene content, including effector genes, within and between core genome lineages suggests the potential for rapid changes to the accessory genome upon dissemination. Evolution of the X. perforans pangenome, including the acquisition and loss of type III secreted effectors, will continue to challenge sustainable management of tomato bacterial spot.

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Section: Core Gene Phylogenymentioning

confidence: 99%

Intercontinental dissemination and diversification ofXanthomonas perforansin tomato production

Timilsina,

Iruegas-Bocardo,

Jibrin

et al. 2024

Preprint

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Focus on the Effectors at the Interface of Plant–Microbe Interactions

Figueroa,

Coaker,

Kanyuka

2024

MPMI

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Comparative transcriptomics reveal a highly polymorphicXanthomonasHrpG virulence regulon

Quiroz Monnens,

Roux,

Cunnac

et al. 2024

Preprint

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Bacteria of the genusXanthomonascause economically significant diseases in various crops. Their virulence is dependent on the translocation of type III effectors (T3Es) into plant cells by the type III secretion system (T3SS), a process regulated by the master response regulator HrpG. Although HrpG has been studied for over two decades, its regulon across diverseXanthomonasspecies, particularly beyond type III secretion, remains understudied. In this study, we conducted transcriptome sequencing to explore the HrpG regulons of 17Xanthomonasstrains, encompassing six species and nine pathovars, each exhibiting distinct host and tissue specificities. We employed constitutive expression of plasmid-bornehrpG*, which encodes a constitutively active form of HrpG, to induce the regulon. Our findings reveal substantial inter- and intra-specific diversity in the HrpG* regulons across the strains. Besides 21 genes directly involved in the biosynthesis of the T3SS, the core HrpG* regulon is limited to only five additional genes encoding the transcriptional activator HrpX, the two T3E proteins XopR and XopL, a major facility superfamily (MFS) transporter, and the phosphatase PhoC. Interestingly, genes involved in chemotaxis and genes encoding enzymes with carbohydrate-active and proteolytic activities are variably regulated by HrpG*. The diversity in the HrpG* regulon suggests that HrpG-dependent virulence inXanthomonasmight be achieved through several distinct strain-specific strategies, potentially reflecting adaptation to diverse ecological niches. These findings enhance our understanding of the complex role of HrpG in regulating various virulence and adaptive pathways, extending beyond T3Es and the T3SS.

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A Community-Curated DokuWiki Resource on Diagnostics, Diversity, Pathogenicity, and Genetic Control of Xanthomonads

Cited by 3 publications

References 38 publications

Intercontinental dissemination and diversification ofXanthomonas perforansin tomato production

Intercontinental dissemination and diversification ofXanthomonas perforansin tomato production

Focus on the Effectors at the Interface of Plant–Microbe Interactions

Comparative transcriptomics reveal a highly polymorphicXanthomonasHrpG virulence regulon

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