A common wild rice-derived BOC1 allele reduces callus browning in indica rice transformation

Zhang, Kun; Su, Jingjing; Xu, Min; Zhou, Zongtan; Zhu, Xiaoyang; Ma, Xin; Hou, Jingjing; Tan, Lubin; Zhu, Zuofeng; Cai, Hongwei; Liu, Fengxia; Sun, Hongying; Gu, Ping; Li, Chen; Liang, Yuntao; Zhao, Wensheng; Sun, Chuanqing; Fu, Yongcai

doi:10.1038/s41467-019-14265-0

Cited by 47 publications

(36 citation statements)

References 75 publications

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“…Under abiotic stress, plants have evolved an antioxidant defence mechanism that protects them against oxidative stress-induced damage by regulating enzymatic and non-enzymatic antioxidant systems (Apel and Hirt, 2004;Gill and Tuteja, 2010;Liu et al, 2014). Previous studies have shown that RCD1 and OsSRO1c are involved in MV stress by influencing the activity of enzymatic antioxidant systems (You et al, 2013;Hiltscher et al, 2014;Zhang et al, 2020b). In this study, we found that the constitutive expression or overexpression of ZmSRO1e accentuated the sensitivity of plants to abiotic stress and promoted the accumulation of ROS (Figures 2 and 3).…”

Section: Function Of Sros In the Abiotic Stress Responsesupporting

confidence: 52%

“…In Oryza sativa (rice), the loss-of-function of OsSRO1c results in plants exhibiting pronounced drought sensitivity, as well as a greater tolerance to oxidative stress (You et al, 2013(You et al, , 2014. Appropriate upregulation of OsSRO1c reduces callus browning and improves the efficiency of genetic transformation; this may be inextricably linked to decreased cell senescence and death caused by oxidative stress (Zhang et al, 2020b). The Triticum aestivum (bread wheat) protein TaSRO1 contributes to abiotic stress tolerance by modulating redox homeostasis and maintaining genomic integrity (Liu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Maize SRO1e represses anthocyanin synthesis through regulating the MBW complex in response to abiotic stress

et al. 2020

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Plants experiencing abiotic stress react by generating reactive oxygen species (ROS), compounds that, if allowed to accumulate to excess, repress plant growth and development. Anthocyanins induced by abiotic stress are strong antioxidants that neutralize ROS, whereas their over-accumulation retards plant growth. Although the mechanism of anthocyanin synthesis has been revealed, how plants balance anthocyanin synthesis under abiotic stress to maintain ROS homeostasis is unknown. Here, ROS-related proteins, SIMILAR TO RCD-ONEs (SROs), were analysed in Zea mays (maize), and all six SRO1 genes were inducible by a variety of abiotic stress agents. The constitutive expression of one of these genes, ZmSRO1e, in maize as well as in Arabidopsis thaliana increased the sensitivity of the plant to abiotic stress, but repressed anthocyanin biosynthesis and ROS scavenging activity. Loss-of-function mutation of ZmSRO1e enhanced ROS tolerance and anthocyanin accumulation. We showed that ZmSRO1e competed with ZmR1 (a core basic helix-loop-helix subunit of the MYB-bHLH-WD40 transcriptional activation complex) for binding with ZmPL1 (a core MYB subunit of the complex). Thus, during the constitutive expression of ZmSRO1e, the formation of the complex was compromised, leading to the repression of genes, such as ZmA4 (encoding dihydroflavonol reductase), associated with anthocyanin synthesis. Overall, the results have revealed a mechanism that allows the products of maize SRO1e to participate in the abiotic stress response.

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Section: Function Of Sros In the Abiotic Stress Responsesupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Maize SRO1e represses anthocyanin synthesis through regulating the MBW complex in response to abiotic stress

et al. 2020

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“…Os01g0182600 encodes the OsGIGANTEA (OsGI) protein, which negatively regulates osmotic stress in rice [ 38 ]. The study revealed that mutation of OsGI led to tolerance to osmotic stress due to increased proline and sucrose contents and accelerated stomatal movement [ 36 ]. These results indicated that decreases in the transcript levels of genes involved in the responses to osmotic stress and phytohormone inactivation were mechanisms that reduced the effects of the stress, thus contributing to the greater tolerance of Dongdao-4 plants.…”

Section: Discussionmentioning

confidence: 99%

“…For the common upregulated genes in roots of both rice genotypes, there were 97 genes with expression levels higher in Dongdao-4 than in Jigeng-88. Among these genes, Os03g0230300, encoding OsSRO1c, confers greater tolerance to drought stress in overexpressing lines through interaction with SNAC1 to promote stomatal closure and reduce water loss [34][35][36].…”

Section: Plos Onementioning

confidence: 99%

Comparative transcriptome analysis of two rice genotypes differing in their tolerance to saline-alkaline stress

Tai

et al. 2020

PLoS ONE

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Saline-alkaline stress is an abiotic stress that suppresses rice plant growth and reduces yield. However, few studies have investigated the mechanism by which rice plants respond to saline-alkaline stress at a global transcriptional level. Dongdao-4 and Jigeng-88, which differ in their tolerance to saline-alkaline stress, were used to explore gene expression differences under saline-alkaline stress by RNA-seq technology. In seedlings of Dongdao-4 and Jigeng-88, 3523 and 4066 genes with differential levels of expression were detected, respectively. A total of 799 genes were upregulated in the shoots of both Dongdao-4 and Jigeng-88, while 411 genes were upregulated in the roots of both genotypes. Among the downregulated genes in Dongdao-4 and Jigeng-88, a total of 453 and 372 genes were found in shoots and roots, respectively. Gene ontology (GO) analysis showed that upregulated genes were enriched in several GO terms such as response to stress, response to jasmonic acid, organic acid metabolic process, nicotianamine biosynthetic process, and iron homeostasis. The downregulated genes were enriched in several GO terms, such as photosynthesis and response to reactive oxygen species. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that Dongdao-4 seedlings were specifically enriched in the biosynthesis of secondary metabolites such as diterpenoids and phenylpropanoids. The upregulated genes that were involved in secondary metabolite biosynthesis, amino acid biosynthesis, betalain biosynthesis, organic acid metabolic process, and iron homeostasis pathways may be central to saline-alkaline tolerance in both rice genotypes. In contrast, the genes involved in the diterpenoid and phenylpropanoid biosynthesis pathways may contribute to the greater tolerance to saline-alkaline stress in Dongdao-4 seedlings than in Jigeng-88. These results suggest that Dongdao-4 was equipped with a more efficient mechanism involved in multiple biological processes to adapt to saline-alkaline stress.

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“…Fourteen additional candidates were put forward based on p -values, annotations, and expression patterns, including a putative thioredoxin, two AP2-domain-containing proteins, and OsIAA10, RNAi knockdown of which impeded callus induction and auxin responsivity [ 102 ]. Lastly, an allele of BROWNING OF CALLUS 1 ( BOC1 ) from wild rice was found to reduce callus browning in indica cultivars by decreasing cell death and senescence in response to oxidative stress [ 103 ].…”

Section: Mapping Natural Variation In the Organogenic Potential Atmentioning

confidence: 99%

Natural Variation in Plant Pluripotency and Regeneration

Lardon

Geelen

2020

Plants

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Plant regeneration is essential for survival upon wounding and is, hence, considered to be a strong natural selective trait. The capacity of plant tissues to regenerate in vitro, however, varies substantially between and within species and depends on the applied incubation conditions. Insight into the genetic factors underlying this variation may help to improve numerous biotechnological applications that exploit in vitro regeneration. Here, we review the state of the art on the molecular framework of de novo shoot organogenesis from root explants in Arabidopsis, which is a complex process controlled by multiple quantitative trait loci of various effect sizes. Two types of factors are distinguished that contribute to natural regenerative variation: master regulators that are conserved in all experimental systems (e.g., WUSCHEL and related homeobox genes) and conditional regulators whose relative role depends on the explant and the incubation settings. We further elaborate on epigenetic variation and protocol variables that likely contribute to differential explant responsivity within species and conclude that in vitro shoot organogenesis occurs at the intersection between (epi) genetics, endogenous hormone levels, and environmental influences.

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A common wild rice-derived BOC1 allele reduces callus browning in indica rice transformation

Cited by 47 publications

References 75 publications

Maize SRO1e represses anthocyanin synthesis through regulating the MBW complex in response to abiotic stress

Maize SRO1e represses anthocyanin synthesis through regulating the MBW complex in response to abiotic stress

Comparative transcriptome analysis of two rice genotypes differing in their tolerance to saline-alkaline stress

Natural Variation in Plant Pluripotency and Regeneration

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