A Common Regulator of Cold and Radiation Response in Escherichia coli

Mangoli, Suhas H.; Sanzgiri, Vibhav R.; Mahajan, Surabhi

doi:10.1615/jenvironpatholtoxicoloncol.v20.i1.40

Cited by 15 publications

(5 citation statements)

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“…In our view their data also suggest that the mean oriC positioning remains essentially unchanged upon migS deletion. It is believed that multiple mechanisms contribute to chromosome segregation: localization to a replication factory (Koppes et al ., 1999), replication which may orient and push the origins in opposite directions (Dingman, 1974), sequestration of the origin by SeqA and binding of SeqA to newly replicated hemimethylated DNA near replication forks (Slater et al ., 1995), condensation of the chromosome by negative superhelicity, MukB and CspE (Hu et al ., 1996; Mangoli et al ., 2001; Nordstrom and Dasgupta, 2001), transertion which is the co‐transcription/translation/insertion of proteins into the membrane (Norris, 1995; Woldringh et al ., 1995), and bipolar migration mediated by migS (Yamaichi and Niki, 2004). A defect in any one step may therefore not have strong effects on chromosome segregation and cell viability, while combining defects may.…”

Section: Discussionmentioning

confidence: 99%

A cis‐acting sequence involved in chromosome segregation in Escherichia coli

Fekete¹,

Chattoraj²

2004

Molecular Microbiology

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SummaryEukaryotic chromosomes contain a locus, the centromere, at which force is applied to separate replicated chromosomes. A centromere analogue is also found in some bacterial plasmids and chromosomes, although not yet identified in the well-studied Escherichia coli chromosome. We aimed to identify centromere-like sequences in E. coli with the premise that such sequences would be the first to migrate towards the cell poles, away from the cell centre where DNA replication is believed to occur. We have labelled different loci on the chromosome by integrating arrays of binding sites for LacI-EYFP and phage l l l l cI-ECFP and supplying these fusion proteins in trans . Comparison of such pairs of loci suggests the presence of a centromere-like site close to the origin of replication. Polar migration of the site was dependent on migS , a locus recently implicated in chromosome migration, thus providing strong support for migS being the E. coli centromere.

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Section: Discussionmentioning

confidence: 99%

A cis‐acting sequence involved in chromosome segregation in Escherichia coli

Fekete¹,

Chattoraj²

2004

Molecular Microbiology

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“…In addition, CspA homologues are involved in diverse phenomena, such as response to freezing conditions, stationary phase, osmotic stress, starvation, antibiotic biosynthesis, resistance to antimicrobial peptides, inhibition of replication, heat resistance of the spores, UV sensitivity etc. [ 171 , 172 , 173 , 174 ]. L. monocytogenes possesses three small, highly homologous protein members of the cold shock protein (Csp) family.…”

Section: Hurdle Technologies and Cross Protectionmentioning

confidence: 99%

Bacterial Stressors in Minimally Processed Food

Capozzi

Fiocco

Amodio

et al. 2009

IJMS

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Stress responses are of particular importance to microorganisms, because their habitats are subjected to continual changes in temperature, osmotic pressure, and nutrients availability. Stressors (and stress factors), may be of chemical, physical, or biological nature. While stress to microorganisms is frequently caused by the surrounding environment, the growth of microbial cells on its own may also result in induction of some kinds of stress such as starvation and acidity. During production of fresh-cut produce, cumulative mild processing steps are employed, to control the growth of microorganisms. Pathogens on plant surfaces are already stressed and stress may be increased during the multiple mild processing steps, potentially leading to very hardy bacteria geared towards enhanced survival. Cross-protection can occur because the overlapping stress responses enable bacteria exposed to one stress to become resistant to another stress. A number of stresses have been shown to induce cross protection, including heat, cold, acid and osmotic stress. Among other factors, adaptation to heat stress appears to provide bacterial cells with more pronounced cross protection against several other stresses. Understanding how pathogens sense and respond to mild stresses is essential in order to design safe and effective minimal processing regimes.

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“…A balanced level of these proteins seems to be important for cold adaptation (48). Among the nine members of this family, CspE performs functions which are important during both cold shock and diverse physiological conditions, including chromosome partitioning/condensation (24,50), growth in cold (30), UV sensitivity (29), and the regulation of various cold-induced genes (36).…”

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confidence: 99%

Cyclic AMP Receptor Protein Regulates cspE , an Early Cold-Inducible Gene, in Escherichia coli

et al. 2011

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cspE, a member of the cspA family of cold shock proteins in Escherichia coli, is an early cold-inducible protein.The nucleic acid melting ability and transcription antiterminator activity of CspE have been reported to be critical for growth at low temperature. Here, we show that the cyclic AMP receptor protein (CRP), a global regulator involved in sugar metabolism, upregulates cspE in E. coli. Sequence analysis of the cspE upstream region revealed a putative CRP target site centered at ؊61.5 relative to the transcription start. The binding of CRP to this target site was demonstrated using electrophoretic mobility shift assays. The presence of this site was shown to be essential for P cspE activation by CRP. Mutational analysis of the binding site indicated that the presence of an intact second core motif is more important than the first core motif for CRP-P cspE interaction. Based on the promoter architecture, we classified P cspE as a class I CRP-dependent promoter. This was further substantiated by our data demonstrating the involvement of the AR1 domain of CRP in P cspE transcription. Furthermore, the substitutions in the key residues of the RNA polymerase ␣-subunit C-terminal domain (␣-CTD), which are important for class I CRP-dependent transcription, showed the involvement of 265 and 287 determinants in P cspE transcription. In addition, the deletion of crp led to a growth defect at low temperature, suggesting that CRP plays an important role in cold adaptation.Escherichia coli K-12 contains nine paralogs of CspA, CspA to CspI, collectively known as the CspA family of cold shock proteins (CSPs). Members of this family are small proteins consisting of a nucleic acid binding domain called the cold shock domain (CSD), one of the most evolutionarily conserved domains found in various life forms, including bacteria, plants, and animals (19,47). In spite of the high degree of similarity among them, only five (cspA, cspB, cspE, cspG, and cspI) are induced during cold shock (20,42). cspC is constitutively expressed at 37°C, while cspD is induced during starvation (49).CspA and some of its homologues share nucleic acid melting ability (25) and transcription antitermination activity (3), two functions that are important for cold adaptation (35). A balanced level of these proteins seems to be important for cold adaptation (48). Among the nine members of this family, CspE performs functions which are important during both cold shock and diverse physiological conditions, including chromosome partitioning/condensation (24, 50), growth in cold (30), UV sensitivity (29), and the regulation of various cold-induced genes (36). cspE expression has been found to be regulated in a growth phase-dependent manner (2). Earlier reports suggested that interplay exists between the cellular metabolic status and cold shock response (13,26,43). Additionally, global gene expression profiling has indicated that many cold-induced genes are subject to catabolite repression (18,34). Cyclic AMP (cAMP) receptor protein (CRP), also known as cata...

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A Common Regulator of Cold and Radiation Response in Escherichia coli

Cited by 15 publications

References 0 publications

A cis‐acting sequence involved in chromosome segregation in Escherichia coli

A cis‐acting sequence involved in chromosome segregation in Escherichia coli

Bacterial Stressors in Minimally Processed Food

Cyclic AMP Receptor Protein Regulates cspE , an Early Cold-Inducible Gene, in Escherichia coli

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