A common organization of the T-DNA genes expressed in plant hairy roots induced by different plasmids of Agrobacterium rhizogenes

Combard, Arlette; Baucher, Marie-France

doi:10.1007/bf00033605

Cited by 9 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1) -were individually cloned in Bin 19 [22] and recombinant plasmids were transferred via triparental mating to the host strain LBA4404 [24]. Data from the only available transcript map [25] and, subsequently, from partial DNA sequencing (J. Brevet, personal communication) allowed us to assign the relative transcript(s) to the cloned T-DNA segments (Fig. 1).…”

Section: Cloning Of Pri 2659 T-dna Segments In Bin19mentioning

confidence: 99%

The non-conserved region of cucumopine-type Agrobacterium rhizogenes T-DNA is responsible for hairy root induction

et al. 1990

View full text Add to dashboard Cite

The T-DNA regions of three strains of Ri plasmids 1855, 8196, 2659 (agropine, mannopine and cucumopine type respectively) share two highly conserved regions flanking a non-homologous central part. We have cloned segments of the cucumopine Ri plasmid 2659 T-DNA in the binary vector system Bin 19 and infected carrot discs with recombinant Agrobacterium strains. We show here that the central non-conserved region is crucial in hairy root induction as it is sufficient to induce rooting on the apical (auxin-rich) surface of carrot discs; in order to observe rooting on the basal (auxin-depleted) side of the discs, a longer T-DNA fragment, also encompassing part of the right conserved region, had to be utilized in conjunction with a Agrobacterium strain carrying aux genes. Differences of growth properties in culture are exhibited by roots transformed with different fragments of pRi 2659 T-DNA, although all transformed roots show the plagiotropic behaviour typical of hairy roots.

show abstract

Section: Cloning Of Pri 2659 T-dna Segments In Bin19mentioning

confidence: 99%

The non-conserved region of cucumopine-type Agrobacterium rhizogenes T-DNA is responsible for hairy root induction

et al. 1990

View full text Add to dashboard Cite

show abstract

“…Agropine and mannopine are also produced by some strains of A. tumefadens (Lahners et al 1984). Formtilae and/or names of a range of opines are given by Combard and Baucher (1988), Petit et al (19S3), Ryder et al (1984) and Szegedi et al (1988).…”

Section: Organization Of Ti and Ri Plasmidsmentioning

confidence: 99%

Structure and function of root‐inducing (Ri) plasmids and their relation to tumor‐inducing (Ti) plasmids

Petersen

Stummann

Olesen³

et al. 1989

Physiologia Plantarum

View full text Add to dashboard Cite

and function of root-inducing (Ri) plasmids and their relation to tumor-inducing (Ti) plasmids. -Physiol. Plant. 77: 427-435.The abilities of Agrobacterium tumefadens and A. rhizogenes to transform dicotyledons and cause erown gall and hairy root disease are caused by the presence of tumor inducing (Ti) and root inducing (Ri) plasmids. During transformation piasmid T-DNA (transferred DNA) is inserted into the plant genome. The T-region is flanked by 25 bp direct repeats, which are essential for transfer. The T-regions contain oncogenes that are expressed in the plants. Some of these code for enzymes that synthesize auxin or cytokinin. Another type, present in Ri plasmids only, appears to impose a high hormone sensitivity on the infected tissue. The T-DNA also contains genes for enzymes synthesizing opines, which the bacteria catabolize. The T-DNA transfer is initiated by the induction of genes in the virulence (vir) region of the piasmid by phenolic compounds secreted by wounded tissue. The products of the W7--genes and of chromosomal genes mediate transfer of T-DNA to the plant cells. Crown gall disease is caused by production of auxin and cytokinin by the transferred T-DNA. The T-DNA of Ri plasmids codes for at least three genes that each can induce root formation, and that together cause hairy root formation from plant tissue. Current results indicate that the products of these genes induce a potential for increased auxin sensitivity that is expressed when the transformed cells are subjected to a certain level of auxin, After this stage the transformed roots can be grown in culture without exogenous supply of hormones.

show abstract

“…The TR-DNA of the agropine-type Ri plasmid contains genes that are homologous to the iaaM and iaaH genes of the Ti T-DNA (Huffman et al 1984). TL-DNA is considered to be essential for the induction of hairy roots, since cucumopine-and mannopine-type Ri plasmids have a single T-DNA that is homologous to the TL-DNA of the agropine-type Ri plasmid (Combard and Baucher 1988). Eighteen open reading frames (ORF) have been identified on the TL-DNA (Slightom et al 1986).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium rhizogenes -mediated DNA transfer to Aesculu s hippocastanum L. and the regeneration of transformed plants

et al. 2004

View full text Add to dashboard Cite

Hairy roots were induced from androgenic embryos of horse chestnut (Aesculus hippocastanum L.) by infection with Agrobacterium rhizogenes strain A4GUS. Single roots were selected according to their morphology in the absence of antibiotic or herbicide resistance markers. Seventy-one putative transformed hairy root lines from independent transformation events were established. Regeneration was induced in MS liquid medium supplemented with 30 microM 6-benzylaminopurine (BA), and the regenerants were multiplied on MS solid medium containing 10 microM BA. Following elongation on MS medium supplemented with 1 microM BA and 500 mg/l polyvinylpyrrolidone, the shoots were subjected to a root-inducing treatment. Stable integration of TL-DNA within the horse chestnut genome was confirmed by Southern hybridization. The copy number of transgenes was estimated to be from two to four.

show abstract

A common organization of the T-DNA genes expressed in plant hairy roots induced by different plasmids of Agrobacterium rhizogenes

Cited by 9 publications

References 26 publications

The non-conserved region of cucumopine-type Agrobacterium rhizogenes T-DNA is responsible for hairy root induction

The non-conserved region of cucumopine-type Agrobacterium rhizogenes T-DNA is responsible for hairy root induction

Structure and function of root‐inducing (Ri) plasmids and their relation to tumor‐inducing (Ti) plasmids

Agrobacterium rhizogenes -mediated DNA transfer to Aesculu s hippocastanum L. and the regeneration of transformed plants

Contact Info

Product

Resources

About