A combined treatment of UV-assisted TiO2 photocatalysis and high hydrostatic pressure to inactivate internalized murine norovirus

Kim, Sun Hyoung; Shahbaz, Hafiz Muhammad; Park, Daseul; Chun, So‐Young; Lee, Wooseong; Oh, Jisun; Lee, Dong Un; Park, Jiyong

doi:10.1016/j.ifset.2016.11.015

Cited by 22 publications

(15 citation statements)

References 46 publications

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“…The failure of both dyes to estimate infectious HAdV after UV disinfection is consistent with studies with PMA/EMA-qPCR for UV-treated bacterial assays [35,57,58] and most likely due to the different mode of virus inactivation (UV breaking down di-hydrogen bonds between nucleotides rather than affecting the virus capsid as described by Beck et al [10]). As for bacteria, similar observations have been described for a variety of enteric viruses (such as MNV, Poliovirus, Norwalk virus, Rotavirus, HAdV, and Coxsackievirus B1) and bacteriophages (like MS2 and PhiX174) after exposure to UV light by [38,[59][60][61]. While culture methods indicated a loss of virus and phage infectivity, the addition of PMA and/or EMA failed to represent this.…”

Section: Resultssupporting

confidence: 68%

Capsid Integrity qPCR—An Azo-Dye Based and Culture-Independent Approach to Estimate Adenovirus Infectivity after Disinfection and in the Aquatic Environment

Leifels

Shoults

Wiedemeyer

et al. 2019

Water

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Recreational, reclaimed and drinking source waters worldwide are under increasing anthropogenic pressure, and often contain waterborne enteric bacterial, protozoan, and viral pathogens originating from non-point source fecal contamination. Recently, the capsid integrity (ci)-qPCR, utilizing the azo-dyes propidium monoazide (PMA) or ethidium monoazide (EMA), has been shown to reduce false-positive signals under laboratory conditions as well as in food safety applications, thus improving the qPCR estimation of virions of public health significance. The compatibility of two widely used human adenovirus (HAdV) qPCR protocols was evaluated with the addition of a PMA/EMA pretreatment using a range of spiked and environmental samples. Stock suspensions of HAdV were inactivated using heat, UV, and chlorine before being quantified by cell culture, qPCR, and ci-qPCR. Apparent inactivation of virions was detected for heat and chlorine treated HAdV while there was no significant difference between ci-qPCR and qPCR protocols after disinfection by UV. In a follow-up comparative analysis under more complex matrix conditions, 51 surface and 24 wastewater samples pre/post UV treatment were assessed for enteric waterborne HAdV to evaluate the ability of ci-qPCR to reduce the number of false-positive results when compared to conventional qPCR and cell culture. Azo-dye pretreatment of non-UV inactivated samples was shown to improve the ability of molecular HAdV quantification by reducing signals from virions with an accessible genome, thereby increasing the relevance of qPCR results for public health purposes, particularly suited to resource-limited low and middle-income settings.

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Section: Resultssupporting

confidence: 68%

Capsid Integrity qPCR—An Azo-Dye Based and Culture-Independent Approach to Estimate Adenovirus Infectivity after Disinfection and in the Aquatic Environment

Leifels

Shoults

Wiedemeyer

et al. 2019

Water

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“…Seventy percent of the articles we researched used PMA or the derived PMAxx, and a third of the studies compared them to EMA and/or the derivate PEMAX ( Table 1 ). In general, PMA and PMAxx were found to be more suitable for capsid integrity qPCR, most likely due to their higher ability to enter thermally and chemically inactivated virions while not penetrating intact capsids or showing microbicidal effects ( Jeong et al., 2017 ; Kim et al., 2017 ; Moreno et al., 2015 ), when compared to infectious virus titers determined by cell culture ( Leifels et al., 2019 ).…”

Section: Azo Dye Type and Concentration Rangementioning

confidence: 99%

Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications – A systematic review

et al. 2021

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“…High hydrostatic pressure (HHP), a non-thermal food processing technique, applies extreme high pressure instead of heat to inactive microorganisms and enzymes while not inducing any chemical changes in flavour and nutrients (Chai et al, 2014;Kim et al, 2017;Shahbaz et al, 2018a). Because HHP is utilised fundamentally by using water as the intermediate substance to transfer the high pressure, it was not regarded as a processing technique for powder products.…”

Section: Introductionmentioning

confidence: 99%

High hydrostatic pressure treatment for manufacturing of garlic powder with improved microbial safety and antioxidant activity

Park

Kim

Shahbaz

et al. 2018

Int J of Food Sci Tech

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Summary The traditional method of manufacturing garlic powder (GP) that includes simple grinding of air‐dried garlic slices has problems of microbial safety and a pungent flavour for this product. Microbiologically safe GP with a less pungent flavour and better antioxidant activities was manufactured using high hydrostatic pressure (HHP), wet grinding and freeze‐drying process. The numbers of total aerobic bacteria and yeasts and molds in untreated (without HHP) GP were 3.64 and 2.47 log CFU/g respectively. Garlic powder treated with 600 MPa HHP for 5 min exhibited a total aerobes count of 1.62 CFU/g and a yeasts and molds count of 1.43 log CFU/g. The diallyl disulfide content, which is responsible for the pungent odour of garlic, was also significantly reduced by HHP due to a decrease in the alliinase activity. Hence, a novel process using HHP can help to produce GP with improved microbial safety, flavour and nutrition.

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A combined treatment of UV-assisted TiO2 photocatalysis and high hydrostatic pressure to inactivate internalized murine norovirus

Cited by 22 publications

References 46 publications

Capsid Integrity qPCR—An Azo-Dye Based and Culture-Independent Approach to Estimate Adenovirus Infectivity after Disinfection and in the Aquatic Environment

Capsid Integrity qPCR—An Azo-Dye Based and Culture-Independent Approach to Estimate Adenovirus Infectivity after Disinfection and in the Aquatic Environment

Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications – A systematic review

High hydrostatic pressure treatment for manufacturing of garlic powder with improved microbial safety and antioxidant activity

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