A combined transient electric birefringence and excluded volume approach to macromolecular shape determination in solution

Harding, Stephen E.

doi:10.1042/bst0140857

Cited by 2 publications

(1 citation statement)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most general shape now available, which can be applied unambiguously and using hydration-independent shape functions, is the general triaxial ellipsoid (i.e., an ellipsoid with three unequal axes). Earlier work Rowe, 1982a, b, 1983;Harding, 1980Harding, , 1986 was concerned with developing much of the necessary methodology. A whole range of analytic expressions are now available enabling us to predict both hydration-dependent and hydration-independent hydrodynamic parameters for any given value of the two axial ratios a/b, b/c, which characterize a general triaxial ellipsoid of semi-axes a 2 b 2 c (Harding, 1982).…”

Section: Introductionmentioning

confidence: 99%

A General Method for Modeling Macromolecular Shape in Solution

Harding¹

1987

Biophysical Journal

Self Cite

View full text Add to dashboard Cite

A general method for modeling macromolecular shape in solution is described involving measurements of viscosity, radius of gyration, and the second thermodynamic virial coefficient. The method, which should be relatively straightforward to apply, does not suffer from uniqueness problems, involves shape functions that are independent of hydration, and models the gross conformation of the macromolecule in solution as a general triaxial ellipsoid. The method is illustrated by application to myosin, and the relevance and applicability of ellipsoid modeling to biological structures is discussed.

show abstract

Section: Introductionmentioning

confidence: 99%