A combined pyrolysis mass spectrometric and light microscopic study of peatified Calluna wood isolated from raised bog peat deposits

Heijden, E. van der; Boon, Jaap J.

doi:10.1016/0146-6380(94)90028-0

Cited by 81 publications

(35 citation statements)

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“…Therefore, oxidation and depolymerization is a major process of the lignin degradation. It has been shown before [36 ], however, that demethoxylation (or selective degradation [47 ]) occurs as well, even to such an extent that only guaiacyl lignin remains. The fungal lignin degradation process appeared less severe after bacterization (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Degradation of lignin by demethoxylation is visualized in the PyMS spectra by a change of the syringyl lignin markers such as m\z 154, 167, 168, 182, 194, 208 and 210 into the guaiacyl ones (m\z 124, 137, 150, 152, 164, 178 and 180) [36 ]. Such a shift, however, can also be explained by selective removal of syringyl-rich secondary cell wall material and retention of guaiacyl-rich middle lamella [47 ]. Oxidation results in a comparative increase of the aldehydes m\z 178 (coniferaldehyde) and 208 (sinapyl aldehyde) and a decrease of the corresponding alcohols m\z 180 and 210.…”

Section: Carnationmentioning

confidence: 99%

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Changes in chemical composition related to fungal infection and induced resistance in carnation and radish investigated by pyrolysis mass spectrometry

Steijl

Niemann

Boon

1999

Physiological and Molecular Plant Pathology

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Pseudomonas fluorescens WCR 417r induces systemic resistance in radish roots challenged by Fusarium oxysporum f. sp. raphani and, incidentally, in carnation stems challenged by Fusarium oxysporum f. sp. dianthi. The induced systemic resistance is not associated with accumulation of pathogenesis-related proteins (PRs) or, at least in case of radish, with increased peroxidase activity. We tested whether the induced systemic resistance might be associated with changes in the host cell wall composition. Cell wall degradation, using pyrolysis mass spectrometry, was demonstrated on fungal infection in carnation and probably in radish as well. Lignin was found to be demethoxylated, oxidized and depolymerized. In carnation both syringyl and guaiacyl lignin were broken down. In radish root probably mainly syringyl lignin was degraded. Cellulose and hemicellulose were degraded in carnation and possibly in radish. Bacterization with P. fluorescens WCS 417r prior to fungal infection reduced such cell wall degradation, consistent with its resistance inducing action. In the case of the radish roots it seems very improbable that this reduction is a necessary component of the induced fungal resistance. For carnation it remains to be investigated.

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Section: Discussionmentioning

confidence: 99%

Section: Carnationmentioning

confidence: 99%

Changes in chemical composition related to fungal infection and induced resistance in carnation and radish investigated by pyrolysis mass spectrometry

Steijl

Niemann

Boon

1999

Physiological and Molecular Plant Pathology

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“…Klap 1997. The utility of this approach has been demonstrated in various studies on the chemical composition of plant tissues (Boon & Haverkamp 1982, Brock et al 1985, Scheijen & Boon 1989, Van der Hage et al 1993, Van der Heijden & Boon 1994, Klap et al 1998.…”

Section: Methodsmentioning

confidence: 99%

Retention of lignin in seagrasses:angiosperms that returned to the sea

Klap¹,

Hemminga²,

Boon³

2000

Mar. Ecol. Prog. Ser.

101

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Using Curie-point Pyrolysis Gas Chromatography Mass Spectrometry [Py-GCMS) and Direct Temperature-resolved Mass Spectrometry (DT-bfS), lignin was detected In highly purified preparations (Milled Wood Lignin = hlWL) of various tissues of the seagrasses Zostera rnarina and Posidonia oceanica. The results ind~cate that P. oceanica contains more lign~n than Z. manna and that roots and rhizomes generally contain more Lignin than leaves. It is concluded that the ability to produce lignin is not lost by the angiosperm ancestors of extant seagrasses upon their colonization of the marine environment. Relative lignin abundances in the different tissues appear to be posltlvely correlated with life span. It is suggested that lignification contributes to the longevity of a tissue by protecting lt against microbial attack, but that deposition of lignin in seagrasses is restricted to tissues that show limited growth.

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“…The ions appearing in the PYMS spectrum were identified by the PYGC/MS analyses of DHPs and by comparing with published data. 3,4,8,11,15,18 Analyses were routinely performed in triplicate.…”

Section: Pyrolysis-mass Spectrometrymentioning

confidence: 99%

Pyrolysis-mass spectrometry analysis of dehydrogenation lignin polymers with various syringyl/guaiacyl ratios

Izumi

Kuroda

1997

Rapid Commun. Mass Spectrom.

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The present paper describes the application of pyrolysis-mass spectrometry (PYMS) to the analyses of lignocellulosic materials. Dehydrogenation lignin polymers (DHPs) with various syringyl/guaiacyl (S/G) ratios, and a standard, milled wood lignin from Aesculus turbinata Blume, were pyrolyzed at 500°C for 4 s and the volatile products were ionized by low-voltage (20 eV) electron impact. The PYMS spectra of the lignins are shown with identification of most mass peaks. Of the observed mass peaks, the intensities of the monomeric peaks corresponding to monomethoxyphenols (m/z 124, 137, 138 and 180) and dimethoxyphenols (m/z 154, 167, 182 and 210) were sensitive to the S/G ratio shifts of the DHPs. Changes in the monomeric mass peak intensities reflected those in the yields of the corresponding products determined by pyrolysis-gas chromatography (PYGC). The S/G pyrolytic ratios, calculated from the summed mass peak intensities of syringyl and guaiacyl monomeric products, showed a linear correlation with syringaldehyde/vanillin (S/V) ratios determined by nitrobenzene oxidation. This finding suggested that the S/V ratios of lignocellulosic materials are predicted from the S/G pyrolytic ratios obtained by PYMS. Similar linear correlations were observed also between the S/G pyrolytic ratios determined by PYGC and the S/V ratios, for the DHPs and for Japanese hardwoods. Unlike the PYGC method, however, the PYMS method does not need time-consuming determinations of the response factor of each product to a flame ionization detector. This advantage may outweigh the inherent problems of PYMS, such as the ambiguous structural assignment of the mass peaks in the quantitative PYMS analyses of lignocellulosic materials. The dimeric mass peaks, which were hardly detected in an analytical system with a gas chromatograph, also contributed to the mass spectra.

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A combined pyrolysis mass spectrometric and light microscopic study of peatified Calluna wood isolated from raised bog peat deposits

Cited by 81 publications

References 50 publications

Changes in chemical composition related to fungal infection and induced resistance in carnation and radish investigated by pyrolysis mass spectrometry

Changes in chemical composition related to fungal infection and induced resistance in carnation and radish investigated by pyrolysis mass spectrometry

Retention of lignin in seagrasses:angiosperms that returned to the sea

Pyrolysis-mass spectrometry analysis of dehydrogenation lignin polymers with various syringyl/guaiacyl ratios

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