1953
DOI: 10.1126/science.118.3053.31-a
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A Combined Method for the Rapid Fixation and Adhesion of Ciliates and Flagellates

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Cited by 40 publications
(14 citation statements)
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“…Other stains used were HE, Weigert's iron-haematoxylin, oil red 0 or fettrot 7B (Ciba) for neutral fat, and the periodic acid-Schiff technique for polysaccharides. Water suspensions of cysts from agar culture, allowed to settle on slides, were fixed with formalin vapour or Nissenbaum's (1953) fixative and stained similarly.…”
Section: Description Of the Amoebaementioning
confidence: 99%
“…Other stains used were HE, Weigert's iron-haematoxylin, oil red 0 or fettrot 7B (Ciba) for neutral fat, and the periodic acid-Schiff technique for polysaccharides. Water suspensions of cysts from agar culture, allowed to settle on slides, were fixed with formalin vapour or Nissenbaum's (1953) fixative and stained similarly.…”
Section: Description Of the Amoebaementioning
confidence: 99%
“…Nissenbaum's fixative (Nissenbaum 1953) was poured over the droplets containing amoebae, and later the amoebae were stained with Weigert's iron haematoxylin (Conn and Darrow 1943) or Kernechtrot (Romeis 1948). Ordinary slides could only be used with difficulty as the organisms adhered poorly to the glass even after 24 hours.…”
Section: Methodsmentioning
confidence: 99%
“…At this stage, the presence of abundant amoebae can again be checked by microscopy. of the block, quickly reversed and flooded with fixative (Nissenbaum, 1953). When, as occasionally happens, the block adheres to the upper slide, it is gently detached with the scalpel blade.…”
Section: Preparation Of Agar Blocksmentioning
confidence: 99%