A Combined Method for the Rapid Fixation and Adhesion of Ciliates and Flagellates

Nissenbaum, Gerald

doi:10.1126/science.118.3053.31-a

Cited by 40 publications

(14 citation statements)

References 2 publications

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“…Other stains used were HE, Weigert's iron-haematoxylin, oil red 0 or fettrot 7B (Ciba) for neutral fat, and the periodic acid-Schiff technique for polysaccharides. Water suspensions of cysts from agar culture, allowed to settle on slides, were fixed with formalin vapour or Nissenbaum's (1953) fixative and stained similarly.…”

Section: Description Of the Amoebaementioning

confidence: 99%

Description of a Naegleria sp. isolated from two cases of primary amoebic meningo‐encephalitis, and of the experimental pathological changes induced by it

Carter

1970

The Journal of Pathology

237

134

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Section: Description Of the Amoebaementioning

confidence: 99%

Description of a Naegleria sp. isolated from two cases of primary amoebic meningo‐encephalitis, and of the experimental pathological changes induced by it

Carter

1970

The Journal of Pathology

237

134

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“…Nissenbaum's fixative (Nissenbaum 1953) was poured over the droplets containing amoebae, and later the amoebae were stained with Weigert's iron haematoxylin (Conn and Darrow 1943) or Kernechtrot (Romeis 1948). Ordinary slides could only be used with difficulty as the organisms adhered poorly to the glass even after 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Chaos zoochlorellaesp. nov. (Gymnamoebia, Amoebidae) from a Danish freshwater pond

Willumsen¹

1982

Journal of Natural History

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“…At this stage, the presence of abundant amoebae can again be checked by microscopy. of the block, quickly reversed and flooded with fixative (Nissenbaum, 1953). When, as occasionally happens, the block adheres to the upper slide, it is gently detached with the scalpel blade.…”

Section: Preparation Of Agar Blocksmentioning

confidence: 99%

A simple method for studying nuclear division in free-living soil amoebae.

Jamieson¹,

Anderson²

1972

J Clin Pathol

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Technical methods alteration in membrane permeability (Jacob, Bischel, and Herschler, 1964). Reversible alteration in membrane protein structure and/or the removal of fatty acids from the cell membrane may occur. These findings imply facilitation of movement of some substances by changes in membrane porosity.As with most staining techniques, it is important that solutions be changed after frequent use. It is also helpful to the interpreter to have a control specimen with known P. carinii stained simultaneously with the test specimen. We found that necropsy lung sections from P. carinnii pneumonitis can be maintained in refrigeration several months for this purpose.The procedure has been carried out with excellent results by technicians after brief orientation.

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A Combined Method for the Rapid Fixation and Adhesion of Ciliates and Flagellates

Cited by 40 publications

References 2 publications

Description of a Naegleria sp. isolated from two cases of primary amoebic meningo‐encephalitis, and of the experimental pathological changes induced by it

Description of a Naegleria sp. isolated from two cases of primary amoebic meningo‐encephalitis, and of the experimental pathological changes induced by it

Chaos zoochlorellaesp. nov. (Gymnamoebia, Amoebidae) from a Danish freshwater pond

A simple method for studying nuclear division in free-living soil amoebae.

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