A Combined In Vitro and In Vivo Assessment of the Safety of the Yeast Strains Kluyveromyces marxianus A4 and A5 Isolated from Korean Kefir

Youn, Hye-Young; Kim, Dong‐Hyeon; Kim, Hyeon-Jin; Jang, Yong-Seok; Song, Kwang‐Young; Bae, Dongryeoul; Kim, Hyunsook

doi:10.1007/s12602-021-09872-7

Cited by 12 publications

(14 citation statements)

References 46 publications

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“…[5][6][7] K. marxianus, a potential probiotic, has been proven to possess anti-inflammatory, anti-oxidant and angiotensin converting enzyme (ACE) inhibitory activities. 14,28,29 There are few studies on the oral administration of K. marxianus for disease treatment. However, several studies have shown that K. marxianus intervention is a practical therapeutic strategy for obesity, type 2 diabetes mellitus (T2DM), and colitis.…”

Section: Discussionmentioning

confidence: 99%

“…† Based on previous research, ethanol-fed mice were treated with K. marxianus (2 × 10 8 CFU), whereas control mice were treated with an equivalent volume (200 µL) of PBS. 14,16,20 The dosage for mice was about 4 × 10 6 CFU per kg body weight, which is equivalent to 2.16 × 10 9 CFU (3.6 × 10 7 CFU kg −1 ) in adult humans (60 kg). It is achievable by consuming commercial dairy products, such as kefir.…”

Section: Animal Experimentationmentioning

confidence: 99%

“…12 The assessment of the safety of K. marxianus PCH 3 97 isolated from kefir was conducted in vitro and in vivo, and the results demonstrated that short-term oral consumption of K. marxianus was safe and well tolerated in a healthy animal model. 13,14 Surprisingly, K. marxianus A4 and A5 decreased the levels of pro-inflammatory cytokines, such as interleukin-1β † Electronic supplementary information (ESI) available. See DOI: https://doi.org/ 10.1039/d3fo01796f a College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Kluyveromyces marxianus supplementation ameliorates alcohol-induced liver injury associated with the modulation of gut microbiota in mice

Cui,

Guo,

Ning

et al. 2023

Food Funct.

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Section: Discussionmentioning

confidence: 99%

Section: Animal Experimentationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Kluyveromyces marxianus supplementation ameliorates alcohol-induced liver injury associated with the modulation of gut microbiota in mice

Cui,

Guo,

Ning

et al. 2023

Food Funct.

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“…Youn et al. ( 2022 ) investigated virulence attributes of two K. marxianus isolates from Korean kefir. They were negative for gelatinase and haemolytic activity but could produce pseudohyphae.…”

Section: Assessmentmentioning

confidence: 99%

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 17: suitability of taxonomic units notified to EFSA until September 2022

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2023

EFS2

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The qualified presumption of safety (QPS) approach was developed to provide a regularly updated generic pre‐evaluation of the safety of microorganisms, intended for use in the food or feed chains, to support the work of EFSA's Scientific Panels. The QPS approach is based on an assessment of published data for each agent, with respect to its taxonomic identity, the body of relevant knowledge and safety concerns. Safety concerns identified for a taxonomic unit (TU) are, where possible, confirmed at the species/strain or product level and reflected by ‘qualifications’. In the period covered by this Statement, new information was found leading to the withdrawal of the qualification ‘absence of aminoglycoside production ability’ for Bacillus velezensis. The qualification for Bacillus paralicheniformis was changed to ‘absence of bacitracin production ability’. For the other TUs, no new information was found that would change the status of previously recommended QPS TUs. Of 52 microorganisms notified to EFSA between April and September 2022 (inclusive), 48 were not evaluated because: 7 were filamentous fungi, 3 were Enterococcus faecium , 2 were Escherichia coli , 1 was Streptomyces spp., and 35 were taxonomic units (TUs) that already have a QPS status. The other four TUs notified within this period, and one notified previously as a different species, which was recently reclassified, were evaluated for the first time for a possible QPS status: Xanthobacter spp. could not be assessed because it was not identified to the species level; Geobacillus thermodenitrificans is recommended for QPS status with the qualification ‘absence of toxigenic activity’. Streptoccus oralis is not recommended for QPS status. Ogataea polymorpha is proposed for QPS status with the qualification ‘for production purposes only’. Lactiplantibacillus argentoratensis (new species) is included in the QPS list.

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“…Several studies have investigated the probiotic attributes of K. marxianus , including its adhesion to the intestinal epithelium, antagonism toward pathogenic bacteria, anti-microbial, and anti-inflammatory functions ( Lane and Morrissey, 2010 ; Liu et al, 2012 ; Romanin et al, 2016 ). Our previous study also evaluated the safety of K. marxianus by characterizing its phenotypic traits associated with virulence, such as the formation of pseudo-hyphae, production of proteinases, and hemolytic activity ( Lim et al, 2019 ; Youn et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Survivability of Kluyveromyces marxianus Isolated From Korean Kefir in a Simulated Gastrointestinal Environment

Youn

Kim

et al. 2022

Front. Microbiol.

Self Cite

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Kluyveromyces marxianus accounts for > 90% of the yeast population of kefir, and recently, its probiotic potential has been actively explored with a focus on its health benefits and safety. Herein, the survivability of five kefir-isolated K. marxianus strains (Km A1–A5) in a simulated gastrointestinal (GI) environment was evaluated and compared with those of commercial probiotic yeast, Saccharomyces boulardii MYA-796. To further explore the potential to survive in the host GI tract, biochemical activities, hydrophobicity assay, biofilm formation, auto-aggregation analysis, and phenol tolerance of the strains were assessed. K. marxianus A4 exhibited the best survivability among all tested strains, including the clinically proven probiotic yeast strain S. boulardii MYA-796 (p = 0.014) in the artificial GI tract ranging from pH 2.0 to 7.5. In addition, the five K. marxianus strains and S. boulardii MYA-796 displayed different assimilation of lactose, xylitol, D-sorbitol, and DL-lactate, indicating that K. marxianus metabolized a wide range of substances and, thus, might be more feasible to nourish themselves in the host GI tract for survival. K. marxianus strains showed a greater hydrophobicity of cell surface, abilities to biofilm formation and auto-aggregation, and phenol tolerance than S. boulardii MYA-796, suggesting greater potential for survival in the host GI tract.

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A Combined In Vitro and In Vivo Assessment of the Safety of the Yeast Strains Kluyveromyces marxianus A4 and A5 Isolated from Korean Kefir

Cited by 12 publications

References 46 publications

Kluyveromyces marxianus supplementation ameliorates alcohol-induced liver injury associated with the modulation of gut microbiota in mice

Kluyveromyces marxianus supplementation ameliorates alcohol-induced liver injury associated with the modulation of gut microbiota in mice

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 17: suitability of taxonomic units notified to EFSA until September 2022

Survivability of Kluyveromyces marxianus Isolated From Korean Kefir in a Simulated Gastrointestinal Environment

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