1985
DOI: 10.1016/0166-0934(85)90084-9
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A combined enzyme immunoassay for rotavirus and adenovirus (EIARA)

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Cited by 94 publications
(62 citation statements)
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“…Considering the last population census carried out by Instituto Brasileiro de Geografia e Estatística (IBGE 2000), the sampling analyzed in relation to the infantile population was regarded as representative in both cities (95% confidence interval). All samples were primarily screened for rotavirus and adenovirus utilizing a combined enzyme immunoassay for Rotavirus A and adenovirus -EIARA - (Pereira et al 1985) and also by polyacrylamide gel electrophoresis -PAGE (Pereira et al 1983). Samples were also screened for calicivirus by RT-PCR using primers designed for the RNA polymerase region (Green et al 1995, Vinjé et al 1997.…”
Section: Methodsmentioning
confidence: 99%
“…Considering the last population census carried out by Instituto Brasileiro de Geografia e Estatística (IBGE 2000), the sampling analyzed in relation to the infantile population was regarded as representative in both cities (95% confidence interval). All samples were primarily screened for rotavirus and adenovirus utilizing a combined enzyme immunoassay for Rotavirus A and adenovirus -EIARA - (Pereira et al 1985) and also by polyacrylamide gel electrophoresis -PAGE (Pereira et al 1983). Samples were also screened for calicivirus by RT-PCR using primers designed for the RNA polymerase region (Green et al 1995, Vinjé et al 1997.…”
Section: Methodsmentioning
confidence: 99%
“…The samples with incompatible results in relation to the two techniques were tested by IEARA, a immunoenzimatic assay for detection of rotavirus and adenovirus antigens (BioManguinhos, RJ, Brazil) (8). This assay is a double sandwich method, in which the antigens are captured by antibodies in goat hiperimune serum, attached to a solid phase.…”
Section: Ferreira Tl Et Almentioning
confidence: 99%
“…All the samples had previously been tested at the Federal University of Piauí by enzyme immunoassay (EIARA-FIOCRUZ) 29 and were found to be positive for rotavirus. Children's Hospital, Melbourne, Australia, and were cultivated in MA104 cells 31 .…”
Section: Methodsmentioning
confidence: 99%