A Combinational Therapy of Articular Cartilage Defects: Rapid and Effective Regeneration by Using Low-Intensity Focused Ultrasound After Adipose Tissue-Derived Stem Cell Transplantation

Song, Byeong‐Wook; Park, Jun‐Hee; Kim, Bomi; Lee, Seahyoung; Lim, Soyeon; Kim, Sang Woo; Choi, Jung-Won; Lee, Jiyun; Kang, Mi-Sun; Hwang, Ki‐Chul; Chae, Dong-Sik; Kim, Il-Kwon

doi:10.1007/s13770-020-00256-6

Cited by 11 publications

(3 citation statements)

References 37 publications

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“…Furthermore, they did not explore any US stimulation. On the other hand, low-intensity pulsed ultrasound stimulation (LIPUS) has been proposed as a tool to promote the chondrogenic differentiation of mesenchymal stem cells (MSCs). , However, it has never been combined with piezoelectric nanomaterials to synergistically direct stem cell fate. It is also worth mentioning that, in general, US stimulations (alone or in synergy with piezoelectric materials) are typically poorly controlled in the state-of-art: in most of the in vitro studies, the setups used for stimulating cells are affected by poorly standardized configurations, lack of proper calibration, and lack of control on US wave reflections/attenuations.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation in Vitro, in Both a Normal and Inflammatory Milieu

Ricotti,

Cafarelli,

Manferdini

et al. 2024

ACS Nano

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The use of piezoelectric nanomaterials combined with ultrasound stimulation is emerging as a promising approach for wirelessly triggering the regeneration of different tissue types. However, it has never been explored for boosting chondrogenesis. Furthermore, the ultrasound stimulation parameters used are often not adequately controlled. In this study, we show that adipose-tissue-derived mesenchymal stromal cells embedded in a nanocomposite hydrogel containing piezoelectric barium titanate nanoparticles and graphene oxide nanoflakes and stimulated with ultrasound waves with precisely controlled parameters (1 MHz and 250 mW/cm 2 , for 5 min once every 2 days for 10 days) dramatically boost chondrogenic cell commitment in vitro. Moreover, fibrotic and catabolic factors are strongly down-modulated: proteomic analyses reveal that such stimulation influences biological processes involved in cytoskeleton and extracellular matrix organization, collagen fibril organization, and metabolic processes. The optimal stimulation regimen also has a considerable anti-inflammatory effect and keeps its ability to boost chondrogenesis in vitro, even in an inflammatory milieu. An analytical model to predict the voltage generated by piezoelectric nanoparticles invested by ultrasound waves is proposed, together with a computational tool that takes into consideration nanoparticle clustering within the cell vacuoles and predicts the electric field streamline distribution in the cell cytoplasm. The proposed nanocomposite hydrogel shows good injectability and adhesion to the cartilage tissue ex vivo, as well as excellent biocompatibility in vivo, according to ISO 10993. Future perspectives will involve preclinical testing of this paradigm for cartilage regeneration.

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Section: Introductionmentioning

confidence: 99%

Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation in Vitro, in Both a Normal and Inflammatory Milieu

Ricotti,

Cafarelli,

Manferdini

et al. 2024

ACS Nano

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“… 2 Research has shown that a lack of blood supply and neuromodulation lead to insufficient self-repair of cartilage joints. 3 Other research has shown that when the diameter of the cartilage injury is >6 mm, the bone wall and articular cartilage around the injury are subsequently damaged. 4 Such damage is observed in patients with severe conditions, eventually leading to osteoarthritis (OA).…”

Section: Introductionmentioning

confidence: 99%

Expression and related mechanisms of miR-330-3p and S100B in an animal model of cartilage injury

Liang²

2021

J Int Med Res

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Objective To investigate the roles of and relationship between microRNA (miR)-330-3p and S100 calcium-binding protein B (S100B) in an animal model of cartilage injury. Methods This study included 30 New Zealand male rabbits randomly divided into three groups: an intervention group, a model group and a sham surgery control group. Modelling was performed in the intervention and model groups, but in the sham surgery group, only the skin was cut. After modelling, the intervention and model groups were injected with the miR-330-3p overexpression vector GV268-miR-330-3p or the control GV268-N-ODN vector, respectively, twice a week for 7 weeks. Results Levels of interleukin-1β and tumour necrosis factor-α in the synovial fluid were significantly higher in the model group than in the intervention and control groups. The level of miR-330-3p in the cartilage tissue was significantly higher in the control group than in the model group but it was significantly lower compared with the intervention group. Levels of S100B, fibroblast growth factor receptor 1 and fibroblast growth factor-2 in the cartilage tissue of rabbits in the model group were significantly higher compared with the control and intervention groups. Conclusion These findings demonstrate that the upregulation of miR-330-3p can inhibit the expression of S100B.

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“…Ultrasound is a non-invasive therapy with significant data supporting its regenerative efficacy in preclinical studies [21,28,[32][33][34]. The effectiveness of ultrasound is dependent on multiple factors such as duty cycle, intensity, duration, frequency, and energy [21,[35][36][37].…”

mentioning

confidence: 99%

Untitled

2020

GJOR

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Context: Musculoskeletal injuries are prevalent in sports, and the application of Sustain Acoustic Medicine (SAM) as a home-use add-on therapy to reduce pain and to increase the probability of athletes returning to sports was evaluated in a case series. Objectives:To examine the improvements in pain and return to function of athletes using SAM in conjunction with traditional therapies after sustaining sports-related musculoskeletal injuries.Introduction: Traditional treatments such as rest, physical therapy, manual therapy, a combination of rest, ice compression, and elevation (RICE) are standard of care for musculoskeletal injuries and do not provide adequate accelerated healing to return athletes to activity. SAM is an FDA-approved bio-regenerative technology, which can provide mechanotransductive and thermal stimuli to accelerate tissue healing and reduction in pain daily. Interventions: A case series of 18 athletes who showed little or no improvement with traditional therapies where prescribed SAM treatment as an add-on daily home-use intervention. The study included athletes with sports musculoskeletal injuries, including the arm/shoulder, upper leg/glutes/hips, knees, back, and foot/ankle. Clinical outcomes were recorded along with the ability of athletes' ability to go back to sports, and satisfaction and usability measures of the home treatment. Results: All athletes were satisfied with the usability and comfort of the therapy and 93%reported the therapy was sufficiently discrete. Clinical outcomes indicate all athletes showed an average pain decrease of 3.33±0.82 (p≤0.05) numerical rating scales (NRS), improvement in function, and quality of life. 87% of the athletes documented an improvement in function, and 55% were able to return to sports after conservative intervention failed. Conclusion: The results of this study indicate that SAM improves athletes' clinical outcomes. Over 50% of athletes were able to return to sports and resume normal daily function after conservative intervention had failed with addition of daily SAM treatment.

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A Combinational Therapy of Articular Cartilage Defects: Rapid and Effective Regeneration by Using Low-Intensity Focused Ultrasound After Adipose Tissue-Derived Stem Cell Transplantation

Cited by 11 publications

References 37 publications

Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation in Vitro, in Both a Normal and Inflammatory Milieu

Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation in Vitro, in Both a Normal and Inflammatory Milieu

Expression and related mechanisms of miR-330-3p and S100B in an animal model of cartilage injury

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