A combination rapid and standard method for identification of Candida albicans

Schaar, Grace; Long, I.; Widra, A.

doi:10.1007/bf02198744

Cited by 9 publications

(7 citation statements)

References 17 publications

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“…The Candida strain was grown on YEPD agar plates at room temperature (38). The isolated organisms were identified as C. albicans by a germ tube test and chlamydospore production as described by Schaar et al (39).…”

Section: Methodsmentioning

confidence: 99%

Effects of Psychological Stress and Fluoxetine on Development of Oral Candidiasis in Rats

Núñez¹,

Novío²,

Suárez³

et al. 2010

Clin Vaccine Immunol

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Psychological stress has been found to suppress cell-mediated immune responses that are important for limiting the proliferation of Candida albicans. Fluoxetine has been observed to reduce negative consequences of stress on the immune system in experimental and clinical models, but there are no data on its effects on oral candidiasis. We designed experiments to evaluate the effects of fluoxetine on the development of oral candidiasis in Sprague-Dawley rats exposed to a chronic auditory stressor. Animals were submitted to surgical hyposalivation in order to facilitate the establishment and persistence of C. albicans infection. Stress application and treatment with drugs (placebo or fluoxetine) were initiated 7 days before C. albicans inoculation and lasted until the end of the experiments, on day 15 postinoculation. Establishment of C. albicans infection was evaluated on days 2 and 15 after inoculation. Tissue injury was determined by the quantification of the number and type (normal or abnormal) of papillae on the dorsal tongue per microscopic field. A semiquantitative scale was devised to assess the degree of colonization of the epithelium by fungal hyphae. Our results showed that stress exacerbates C. albicans infection in the tongues of rats. Significant increases in Candida counts, the percentage of the tongue's surface covered with clinical lesions, the percentage of abnormal papillae, and the colonization of the epithelium by hyphae were found in stressed rats compared to the nonstressed ones. Treatment with fluoxetine significantly reversed these adverse effects of stress. Besides the psychopharmacological properties of fluoxetine against stress, it has consequences for Candida infection.

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Section: Methodsmentioning

confidence: 99%

Effects of Psychological Stress and Fluoxetine on Development of Oral Candidiasis in Rats

Núñez¹,

Novío²,

Suárez³

et al. 2010

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…The Candida strains were grown on YEPD agar plates at room temperature (35). The isolated organisms were identified as C. albicans by a germ tube test and chlamydospore production as described by Schaar et al (36).…”

Section: Methodsmentioning

confidence: 99%

Effects of Psychological Stress and Alprazolam on Development of Oral Candidiasis in Rats

Núñez

Balboa

Riveiro

et al. 2002

Clin Vaccine Immunol

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Psychological stress has been found to suppress cell-mediated immune responses that are important in limiting the proliferation of Candida albicans. Since anxiolytic drugs can restore cellular immunity in rodents exposed to stress conditions, we designed experiments conducted to evaluate the effects of alprazolam (1 mg/kg of body weight/day), a central benzodiazepine anxiolytic agonist, on the development of oral candidiasis in Sprague-Dawley rats exposed to a chronic auditory stressor. Animals were submitted to surgical hyposalivation in order to facilitate the establishment and persistence of C. albicans infection. Application of stress and treatment with drugs (placebo or alprazolam) were initiated 7 days before C. albicans inoculation and lasted until the end of the experiments (day 15 postinoculation). Establishment of C. albicans infection was evaluated by swabbing the inoculated oral cavity with a sterile cotton applicator on days 2 and 15 after inoculation, followed by plating on YEPD (yeast extract-peptone-dextrose) agar. Tissue injury was determined by the quantification of the number and type (normal or abnormal) of papillae on the dorsal tongue per microscopic field. A semiquantitative scale was devised to assess the degree of colonization of the epithelium by fungal hyphae. Our results show that stress exacerbates C. albicans infection of the tongues of rats. Significant increases in Candida counts, the percentage of the tongue's surface covered with clinical lesions, the percentage of abnormal papillae, and the colonization of the epithelium by fungal hyphae were found in stressed rats compared to those found in the unstressed rats. Treatment with alprazolam significantly reversed these adverse effects of stress, showing that, besides the psychopharmacological properties of this anxiolytic drug against stress, it has consequences for Candida infection.

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“…The Candida strain was grown on YEPD agar plates at room temperature (27). The isolated organisms were identified as C. albicans by a germ tube test and chlamydospore production as described by Schaar et al (28).…”

Section: Methodsmentioning

confidence: 99%

Effects of Amphetamine on Development of Oral Candidiasis in Rats

Freire‐Garabal¹,

Núñez²,

Balboa³

et al. 1999

Clin Diagn Lab Immunol

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Experiments were conducted to evaluate the effects of amphetamine (0.4 mg/kg of body weight/day) on the development of oral candidiasis in Sprague-Dawley rats. Animals were submitted to surgical hyposalivation in order to facilitate the establishment and persistence of Candida albicans infection. Treatment with drugs (placebo or amphetamine) was initiated 7 days before C. albicans inoculation and lasted until the end of the experiments, day 15 postinoculation. Establishment of C. albicansinfection was evaluated by swabbing the inoculated oral cavity with a sterile cotton applicator on days 2 and 15 after inoculation, followed by plating on YEPD (yeast extract-peptone-dextrose) agar. Tissue injury was determined by the quantification of the number and type (normal or abnormal) of papillae on the dorsal tongue per microscopic field. A semiquantitative scale was devised to assess the degree of colonization of the epithelium by fungal hyphae. Our results show that amphetamine exacerbates C. albicans infection of the tongues of rats. Significant increases in Candida counts, the percentage of the tongue’s surface covered with clinical lesions, the percentage of abnormal papillae, and the colonization of the epithelium by fungal hyphae were found in amphetamine-treated rats compared to those found in the rats injected with a placebo. The last two parameters increased in rats treated with the placebo compared to the parameters of the untreated control rats.

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A combination rapid and standard method for identification of Candida albicans

Cited by 9 publications

References 17 publications

Effects of Psychological Stress and Fluoxetine on Development of Oral Candidiasis in Rats

Effects of Psychological Stress and Fluoxetine on Development of Oral Candidiasis in Rats

Effects of Psychological Stress and Alprazolam on Development of Oral Candidiasis in Rats

Effects of Amphetamine on Development of Oral Candidiasis in Rats

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