A combination of scGOS/lcFOS with Bifidobacterium breve M-16V protects suckling rats from rotavirus gastroenteritis

Rigo-Adrover, Maria Del Mar; Saldaña-Ruíz, Sandra; Limpt, Kees van; Knipping, Karen; Garssen, Johan; Knol, Jan; Franch, Àngels; Castell, Margarida; Pérez-Cano, Francisco J.

doi:10.1007/s00394-016-1213-1

Cited by 42 publications

(104 citation statements)

References 88 publications

(115 reference statements)

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“…Diarrhea was studied in terms of severity and incidence variables ( Figure 2 and Table 2). RV infection resulted in a mild diarrhea in suckling rats, with DI scores around 2-3, showing the peak of severity (MSd) and incidence (MId) on day 8 and an overall prevalence of diarrhea (%DA) of 88.9%, similarly to previous studies [13,56]. The B. breve M-16V, L. acidophilus NCFM, and L. helveticus R0052 groups displayed clinical amelioration of severity (DI) and incidence (%DA) during the peak of The analysis of morphometric variables was performed at the end of the study (Table 1).…”

Section: Clinical Evaluation Of Diarrheasupporting

confidence: 88%

“…Suckling rats received oral administration once daily, as previously described [13], with the same normalized volume/body weight of vehicle or probiotics (5.5 µL/g/day), from days 2-14 of life, corresponding to the strict lactation period. The probiotics were administered at a dose of 1 × 10 9 CFU/100 g/day.…”

Section: Experimental Designmentioning

confidence: 99%

“…The disease burden of RV is substantial in both developed and developing countries; in fact, almost every child has been infected by the age of 5, and probably more than once, because reinfections are frequent [10][11][12]. Although RV vaccines are available to prevent the infection, they are not globally implemented due to their high cost, cold storage requirements, and the lower protection they offer in developing countries as a consequence of malnutrition, micronutrient deficiencies, and suboptimal breastfeeding [13,14]. Treatment of RV diarrhea mainly relies on oral rehydration solutions to replace fluid loss.…”

Section: Introductionmentioning

confidence: 99%

“…However, before moving to clinical trials, the safety and efficacy of probiotics need to be proven in preclinical models. In this sense, preclinical evidence has been accumulated for the following species: Lactobacillus casei [32][33][34], Lactobacillus plantarum [31], Lactobacillus rhamnosus GG [5,14,26,35], Bifidobacterium adolescentis [34], Bifidobacterium bifidum [29], Bifidobacterium infantis [29], Bifidobacterium breve [13,36], and Bifidobacterium lactis [35]. In addition, these previous studies led to the further study of the clinical evidence for the following species: Lactobacillus acidophilus [16,20,32,37], L. casei [26], Lactobacillus reuteri [38,39], L. rhamnosus GG [16,17,20,22,[40][41][42][43], Lactobacillus sporogenes [44,45], B. lactis [46][47][48], and Bifidobacterium longum [16,20,37].…”

Section: Introductionmentioning

confidence: 99%

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Strain-Specific Probiotic Properties of Bifidobacteria and Lactobacilli for the Prevention of Diarrhea Caused by Rotavirus in a Preclinical Model

et al. 2020

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Probiotic supplementation with different lactobacilli and bifidobacterial strains has demonstrated beneficial effects in infectious diarrhea caused by rotavirus (RV) in young children. Preclinical models of RV infection might be a good strategy to screen for the efficacy of new probiotic strains or to test their comparative efficacy. Neonatal Lewis rats were supplemented with Bifidobacterium breve M-16V, Lactobacillus acidophilus NCFM, Lactobacillus helveticus R0052, or Lactobacillus salivarius PS2 from days 2–14 of life. On day five, animals received RV SA-11 orally. Fecal samples were collected daily, weighed, and scored for the calculation of severity and incidence of diarrhea. In addition, fecal pH and fecal viral shedding were measured. Animals were sacrificed at the end of the study and their blood was obtained for the quantification of RV-specific immunoglobulins. RV infection was induced in ~90% of the animals. All probiotics caused a reduction of several clinical variables of severity and incidence of diarrhea, except L. salivarius PS2. L. acidophilus NCFM, B. breve M-16V, and L. helveticus R0052 seemed to be very effective probiotic strains. In addition, all Lactobacillus strains reduced the viral elimination one day post-inoculation. No differences were detected in the specific anti-RV humoral response. The present study highlights the strain-specific effects of probiotics and identifies promising probiotics for use in ameliorating and preventing RV-induced diarrhea in children, for example by including them in infant formulas.

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Section: Clinical Evaluation Of Diarrheasupporting

confidence: 88%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Strain-Specific Probiotic Properties of Bifidobacteria and Lactobacilli for the Prevention of Diarrhea Caused by Rotavirus in a Preclinical Model

et al. 2020

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“…This probiotic seems to ameliorate allergy symptoms, often in combination with prebiotics [26–29], and rotavirus diarrhoea [30]. The aim of the present study was to evaluate the impact of B .…”

Section: Introductionmentioning

confidence: 99%

Preclinical Immunomodulation by the Probiotic Bifidobacterium breve M-16V in Early Life

et al. 2016

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This study aimed to investigate the effect of supplementation with the probiotic Bifidobacterium breve M-16V on the maturation of the intestinal and circulating immune system during suckling. In order to achieve this purpose, neonatal Lewis rats were supplemented with the probiotic strain from the 6th to the 18th day of life. The animals were weighed during the study, and faecal samples were obtained and evaluated daily. On day 19, rats were euthanized and intestinal wash samples, mesenteric lymph node (MLN) cells, splenocytes and intraepithelial lymphocytes (IEL) were obtained. The probiotic supplementation in early life did not modify the growth curve and did not enhance the systemic immune maturation. However, it increased the proportion of cells bearing TLR4 in the MLN and IEL, and enhanced the percentage of the integrin αEβ7+ and CD62L+ cells in the MLN and that of the integrin αEβ7+ cells in the IEL, suggesting an enhancement of the homing process of naïve T lymphocytes to the MLN, and the retention of activated lymphocytes in the intraepithelial compartment. Interestingly, B. breve M-16V enhanced the intestinal IgA synthesis. In conclusion, supplementation with the probiotic strain B. breve M-16V during suckling improves the development of mucosal immunity in early life.

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Prebiotics and their Role in Functional Food Product Development

Panwar¹,

Panesar²,

Saini³

2022

Probiotics, Prebiotics and Synbiotics

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A combination of scGOS/lcFOS with Bifidobacterium breve M-16V protects suckling rats from rotavirus gastroenteritis

Abstract: A daily supplement of a scGOS/lcFOS 9:1 prebiotic mixture, Bifidobacterium breve M-16V or a combination of both is highly effective in modulating RV-induced diarrhoea in this preclinical model.

Cited by 42 publications

References 88 publications

Strain-Specific Probiotic Properties of Bifidobacteria and Lactobacilli for the Prevention of Diarrhea Caused by Rotavirus in a Preclinical Model

Strain-Specific Probiotic Properties of Bifidobacteria and Lactobacilli for the Prevention of Diarrhea Caused by Rotavirus in a Preclinical Model

Preclinical Immunomodulation by the Probiotic Bifidobacterium breve M-16V in Early Life

Prebiotics and their Role in Functional Food Product Development

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