A combination of resveratrol and quercetin induces browning in white adipose tissue of rats fed an obesogenic diet

Abstract

Objective: To analyze whether a combination of quercetin (Q) and resveratrol (RSV) would induce a white adipose tissue (WAT) browning effect. Methods: Thirty-six rats were fed an obesogenic diet and divided into four groups: control, treated with RSV (15 mg/kg body weight/day; RSV group), treated with Q (30 mg/kg body weight/day; Q group), or treated with both polyphenols (RSV 1 Q group). Results: After 6 weeks, body and WAT weights were significantly reduced in the RSV 1 Q group. In perirenal WAT of the control, RSV, and Q groups, white unilocular adipocytes appeared in the majority of cells, while in the RSV 1 Q group numerous multilocular adipocytes with positive immunostaining for UCP1 were observed. The presence of UCP1 was confirmed by Western blot. This group also revealed increased mRNA levels of Cidea, Hocx9, Bmp4, Slc27a1, Pat2, Atgl, and Atp5d. Interscapular brown adipose tissue weight showed no differences between groups, but the Cidea mRNA level was increased in the RSV group, the Cox-2 mRNA level in the RSV 1 Q group, and UCP1 protein expression in the RSV and the RSV 1 Q groups. Conclusions: This study demonstrated that the RSV 1 Q combination produces a brown-like remodeling effect in perirenal WAT, as well as increased UCP1 protein expression in interscapular brown adipose tissue.

Resveratrol promotes white adipocytes browning and improves metabolic disorders in Sirt1‐dependent manner in mice

“…Res improves insulin sensitivity and ameliorates insulin resistance in KKAy mice, which is likely associated with upregulation of Sirt1 protein in liver and soleus muscles and consequent AMPK activation . Finally, several reports reveal that Res is able to induce beiging in WATs …”

“…15,16 Finally, several reports reveal that Res is able to induce beiging in WATs. [17][18][19] However, how Res regulates the metabolism of adipose tissue and WAT browning remains mostly elusive, and whether its metabolites are involved in this process is poorly understood. The current study first illustrated that Res reduced fat accumulation in 3T3-L1 adipocytes with insulin resistance via Sirt1 pathway.…”

Synergistic Effect of Bupleuri Radix and Scutellariae Radix on Adipogenesis and AMP‐Activated Protein Kinase: A Network Pharmacological Approach

“…Zhao et al used HFD-induced rats to show synergism between quercetin and resveratrol [38]. Another group has also reported the synergistic effect of this pair “browning” of white adipose tissue [39]. In this study, we first analyzed the possible synergy of BR-SR pair by a network pharmacological approach.…”

Antiobesity effects of resveratrol: which tissues are involved?

“…These results were accompanied by increased protein expression of PRDM16 and cytochrome c, two markers of brown adipogenesis . By contrast, in a study carried out in our laboratory, resveratrol was not able to induce browning in inguinal or perirenal white adipose depots from rats fed a high‐fat diet and treated with resveratrol at a dose of 15 mg/kg/day . An important factor that can explain, at least in part, the discrepancy between these two studies is that the mouse may be a more sensitive responder than the rat.…”

“…An important factor that can explain, at least in part, the discrepancy between these two studies is that the mouse may be a more sensitive responder than the rat. Recently, several cases of white adipose tissue browning induced by resveratrol derivatives, such as BTM‐0512, or resveratrol combinations, such as resveratrol and quercetin, have been reported. Nevertheless, this review focuses only on resveratrol.…”