A Combination of Methylation and Protein Markers is Capable of Detecting Gastric Cancer&amp;#x00C2;&amp;#x00A0;Detection by Combined Markers

Xu, Jing; Song, Jianlin; Wang, Tongmin; Zhu, Wenchuan; Zuo, Liangyu; Wu, Jinzhi; Guo, Jun; Yang, Xiaochun

doi:10.2217/epi-2021-0080

Cited by 14 publications

(15 citation statements)

References 45 publications

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“…For the initial review, 95 relevant pieces of literature were obtained. Following the layer-by-layer screening, 9 studies on diagnostic accuracy with 1531 subjects were finally included (2,11,22,(24)(25)(26)(27)(28)(29). Figure 1 depicts the screening process and results of the literature.…”

Section: Search Resultsmentioning

confidence: 99%

Diagnostic value of plasma RNF180 gene methylation for gastric cancer: A systematic review and meta-analysis

Zhang

Wang

2023

Front. Oncol.

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ObjectiveA systematic evaluation of the diagnostic value of Ring finger protein 180 (RNF180) gene methylation as a novel tumor marker for gastric cancer (GC) is required to improve the early diagnosis of gastric cancer patients.MethodsComputer searches of PubMed, Web of Science, Embase, The Cochrane Library, CNKI, CBM, WanFang Data, National Research Register, Cclinical Controlled Trials, Opengrey and VIP databases were conducted from the database’s inception to September 1, 2022. Two researchers independently screened the literature, extracted information, and assessed the risk of bias in studies that were included. The meta-analysis was carried out using RevMan 5.3 and Stata 16.0 software.ResultsA total of 9 studies with a total of 1531 subjects were included. A random-effects meta-analysis revealed that the combined sensitivity (SEN), specificity (SPE), positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) of plasma RNF180 gene methylation for the diagnosis of GC were: 0.54 [95% CI (0.45, 0.62)], 0.80 [95% CI (0.72, 0.87)], 2.73 [95% CI (2.09, 3.57)], 0.58 [95% CI (0.51, 0.65)], 4.74 [95% CI (3.59, 6.62)], respectively.ConclusionThe detection of RNF180 gene methylation in plasma has a high diagnostic value for GC and is expected to be a potential biomarker for the diagnosis of gastric cancer, according to current evidence. Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=370903, identifier CRD42022370903.

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Section: Search Resultsmentioning

confidence: 99%

Diagnostic value of plasma RNF180 gene methylation for gastric cancer: A systematic review and meta-analysis

Zhang

Wang

2023

Front. Oncol.

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“…Moreover, the panel developed in our study also displayed a comparable sensitivity for each UGC type. For example, Xu et al reported a combined methylation assay for GC detection with a sensitivity of 60.3% and a specificity less than 88% [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

A Novel Plasma-Based Methylation Panel for Upper Gastrointestinal Cancer Early Detection

Peng

Zhao

Pei

et al. 2022

Cancers

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Background: Upper gastrointestinal cancer (UGC) is an important cause of cancer death in China, with low five-year survival rates due to the majority of UGC patients being diagnosed at an advanced stage. Therefore, there is an urgent need to develop cost-effective, reliable and non-invasive methods for the early detection of UGC. Methods: A novel plasma-based methylation panel combining simultaneous detection of three methylated biomarkers (ELMO1, ZNF582 and TFPI2) and an internal control gene were developed and used to examine plasma samples from 186 UGC patients and 190 control subjects. Results: The results indicated excellent PCR amplification efficiency and reproducibility of ELMO1, ZNF582 and TFPI2 in the range of 10–100,000 copies per PCR reaction of fully methylated genomic DNA. The methylation levels of ELMO1, ZNF582 and TFPI2 were significantly higher in UGC samples than those in control subjects. The sensitivities of ELMO1, ZNF582 and TFPI2 alone for UGC detection were 32.3%, 61.3% and 30.6%, respectively; when three markers were combined, the sensitivity was improved to 71.0%, with a specificity of 90.0%, and the area under the curve (AUC) was 0.870 (95% CI: 0.832–0.902). Conclusion: Methylated ELMO1, ZNF582 and TFPI2 were specific for UGC and the three-methylated gene panel provided an alternative non-invasive choice for UGC early detection.

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“…Yongzhan Nie 1,# Xianchun Gao 1,# Xiqiang Cai 2,# Zhen Wu 3,# Qiaoyi Liang 4 Guobing Xu 5 Na Liu 6 Peng Gao 7 Jingyu Deng 8 Hongzhi Xu 9 Zhanlong Shen 10 Changqi Cao 5 Fenrong Chen 6 Nannan Zhang 1 Yongxi Song 7 Mingjun Sun 11 Chengyin Liu 3 Guangpeng Zhou 3 Weili Han 1 Jianhua Dou 1 Huahong Xie 1 Liping Yao 1 Zhiguo Liu 1 Gang Ji 1 Xin Wang 2 Qingchuan Zhao 1 Lei Shang 12 Daiming Fan 1 Xiaoliang Han 3 Jianlin Ren 9 Han Liang 8 Zhenning Wang 7 Jinhai Wang…”

Section: E T H I C S a P P R O Va L A N D C O N S E N T T O Pa R T I ...mentioning

confidence: 99%

Combining methylated SEPTIN9 and RNF180 plasma markers for diagnosis and early detection of gastric cancer

Nie

Gao

Cai

et al. 2023

Cancer Communications

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Dear Editor, Early diagnosis is critical for successful treatment of gastric adenocarcinoma (GA). However, the sensitivities of tumor markers carcinoembryonic antigen (CEA), cancer antigen 19-9 (CA19-9) and CA72-4 for GA detection are approximately 20% [1], and the sensitivities of all markers combined for early gastric cancer detection is still very low [2]. DNA methylation plays a major role in tumorigenesis and therefore has obvious potential as a non-invasive biomarker for cancer detection [3]. Through genome-wide methylation analysis and histological verification, we previously identified ring finger protein 180 (RNF180) as a novel preferentially methylated gene in GA [4,5].To increase the sensitivity for detecting GA, we combined mRNF180 and other methylated DNA markers. According to previous studies, the sensitivity and specificity of circulating methylated SEPTIN9 (mSEPT9) are estimated to be 50%-70% and ≥ 90%, respectively, to detect colorectal cancer (CRC) [6,7]. GA and CRC share similar biological features. Notably, GA and CRC share many similar aberrant promoter DNA methylations, resulting in sharing many consistent gene methylation biomarkers [8]. Therefore, we attempted to establish a panel of mRNF180 and mSEPT9 (RS9 panel) for the early detection of GA. The study protocols are included in the Supplementary Materials.To assess the diagnostic potential of the RS9 panel, we prospectively examined 324 plasma specimens from 195 GA patients and 129 controls in the training cohort

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A Combination of Methylation and Protein Markers is Capable of Detecting Gastric CancerÂ Detection by Combined Markers

Cited by 14 publications

References 45 publications

Diagnostic value of plasma RNF180 gene methylation for gastric cancer: A systematic review and meta-analysis

Diagnostic value of plasma RNF180 gene methylation for gastric cancer: A systematic review and meta-analysis

A Novel Plasma-Based Methylation Panel for Upper Gastrointestinal Cancer Early Detection

Combining methylated SEPTIN9 and RNF180 plasma markers for diagnosis and early detection of gastric cancer

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