A Combination of Linalool, Vitamin C, and Copper Synergistically Triggers Reactive Oxygen Species and DNA Damage and Inhibits
 Salmonella enterica
 subsp.
 enterica
 Serovar Typhi and
 Vibrio fluvialis

Ghosh, Tapash Chandra; Srivastava, Santosh K.; Gaurav, Abhishek; Kumar, Alok; Kumar, Piyush; Yadav, A. S.; Pathania, Ranjana; Navani, Naveen Kumar

doi:10.1128/aem.02487-18

Cited by 28 publications

(23 citation statements)

References 64 publications

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“…The most probable Vit C antibacterial mechanism of action is based on oxidative stress due to the generation of ROS. According to a very recent study [33], the combination of Vit C with an essential oil and copper resulted in free radical generation through the Fenton reaction. Honey, particularly honeydew honey, is rich in minerals including transition metal ions (Fe and Cu) and polyphenols, and its combination with Vit C may trigger the intracellular production of ROS in bacterial cells.…”

Section: Discussionmentioning

confidence: 99%

Vitamin C Enhances the Antibacterial Activity of Honey against Planktonic and Biofilm-Embedded Bacteria

et al. 2020

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Multifactorial antibacterial action is an important feature of honey; however, its bactericidal efficacy against biofilm-embedded bacteria is limited. The aim of this study was to investigate the impact of vitamin C (Vit C) on the antibacterial activity of natural honeys against planktonic as well as biofilm-embedded bacterial pathogens. The antibacterial activity of four honey samples supplemented with Vit C was expressed as the minimum inhibitory concentration (MIC). At sub-MICs, Vit C significantly increased the antibacterial activity of the tested honeys against Pseudomonas aeruginosa in planktonic cultures. However, after supplementation, honeydew honey, the most active honey, was ineffective against Staphylococcus aureus. On the other hand, when 100% honeydew honey was supplemented with Vit C (100 mg/g of honey) in a multispecies wound biofilm model, complete eradication of almost all bacterial isolates, including S. aureus, was observed. Furthermore, a mixture of honey and Vit C was partially effective against Enterococcus faecalis, whereas honey alone exhibited no antibacterial activity against this bacterium. Vit C counteracted hydrogen peroxide in honey solution and, thus, eliminated the major antibacterial compound present in honey. It is likely that a combination of honey with Vit C may trigger the intracellular production of reactive oxygen species in bacterial cells, but the exact cellular mechanisms warrant further investigations.

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Section: Discussionmentioning

confidence: 99%

Vitamin C Enhances the Antibacterial Activity of Honey against Planktonic and Biofilm-Embedded Bacteria

et al. 2020

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“…Furthermore, a recent study revealed that the antibacterial effect of vitamin C against S. enterica subsp. enterica serovar Typhi and Vibrio fluvialis could be enhanced when applied in a combination with linalool and copper [113]. The effect of this triple combination on bacterial morphology was demonstrated by scanning electron microscopy using V. fluvialis , which showed severe membrane damage, whereas no toxicity could be assessed in human embryonic kidney (HEK293) cells at synergistic concentrations (16.3 μM, 8 mM, and 1.298 mM vitamin C) [113].…”

Section: Anti-salmonella Effects Of Vitamin Cmentioning

confidence: 99%

Immunomodulatory and antimicrobial effects of vitamin C

Mousavi

Bereswill

Heimesaat

2019

EuJMI

191

151

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Humans have lost their vitamin C-synthesizing capacities during evolution. Therefore, the uptake of this essential compound from external sources is mandatory in order to prevent vitamin C-deficient conditions resulting in severe morbidities such as scurvy. The potent antioxidant, immunomodulatory, and antiinfectious effects of vitamin C are known since the 1930s. We here (i) review the impact of vitamin C on innate and adaptive immune functions, (ii) provide an overview of its antimicrobial, antibacterial, antiviral, antiparasitic, and antifungal properties, and finally, (iii) discuss vitamin C as an adjunct treatment option for the combat of human infections by bacteria, particularly by emerging multidrug-resistant species.

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“…Chickens possess the ability to synthesize VC in kidneys through the glucuronatexylulose-xylulose cycle in the presence of L-gulonolactone oxidase ([EC 1.1.3.8], GLO ). Several researches were reported that VC alone or with other components could decrease SE ( Hernandez-Patlan et al., 2018 ) or other Salmonella species ( Tamoghna Ghosh et al., 2019 ) in vitro. Hernandez-Patlan et al.…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with vitamin C ameliorates the adverse effects of Salmonella Enteritidis-challenge in broilers by shaping intestinal microbiota

et al. 2020

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Salmonella Enteritidis ( SE ) infection is not only a leading cause of poor production performance and compromised animal welfare in broilers but also a potential threat to public health. Two experiments were conducted to evaluate the effects of dietary supplemental vitamin C ( VC ) on SE challenged-broilers. In experiment 1, one hundred eighty 1-day-old Arbor Acre broilers were randomly allocated into 3 treatments, with 0, 500, or 1,000 mg/kg VC included in the diet. In experiment 2, dietary VC at 0 or 500 mg/kg, with or without SE challenge was applied in a 2 × 2 factorial arrangement in 6 randomized complete blocks. In experiment 1, addition with 500 mg/kg VC increased BW and infectious bursal disease ( IBD ) titer of broilers on 35 D ( P < 0.05), whereas 1,000 mg/kg VC had no effects on the IBD titer ( P > 0.05) compared with the control group. In experiment 2, SE challenge depressed BW on 11 and 21 D ( P < 0.05 and P = 0.088, respectively), whereas increased mortality and hepatic bacterial translocation ( P < 0.05) on 21 D. Further, SE challenge resulted in lower villus height in jejunum, lower microbial richness, and diversity, whereas higher abundance of Enterobacteriaceae in cecum ( P < 0.05). Importantly, supplementation with VC increased BW on both 21 and 35 D ( P < 0.05 and P = 0.088, respectively) and enhanced the intestinal health by improving villus morphology and microbial structure as indicated by higher cecal microbial richness and Firmicutes to Bacteroidetes ratio, while lower abundance of Enterobacteriaceae ( P < 0.05). In addition, birds fed with 500 mg/kg VC in the diet had significantly increased jejunal secretory immunoglobulin A levels, T lymphocytes stimulation index, and serum total antioxidant capability compared with groups without VC ( P < 0.05). In conclusion, SE challenge induced lower production performance and higher mortality in broilers. However, dietary supplementation with VC ameliorated SE-caused damage in broilers by improving the intestinal health, partly mediated by shaping the structure of cecal microbiota.

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A Combination of Linalool, Vitamin C, and Copper Synergistically Triggers Reactive Oxygen Species and DNA Damage and Inhibits Salmonella enterica subsp. enterica Serovar Typhi and Vibrio fluvialis

Cited by 28 publications

References 64 publications

Vitamin C Enhances the Antibacterial Activity of Honey against Planktonic and Biofilm-Embedded Bacteria

Vitamin C Enhances the Antibacterial Activity of Honey against Planktonic and Biofilm-Embedded Bacteria

Immunomodulatory and antimicrobial effects of vitamin C

Dietary supplementation with vitamin C ameliorates the adverse effects of Salmonella Enteritidis-challenge in broilers by shaping intestinal microbiota

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