A colorimetric zinc(II) assay based on the use of hairpin DNAzyme recycling and a hemin/G-quadruplex lighted DNA nanoladder

Zhu, Longjiao; Gui-shan, LI; Shao, Xiaofeng; Huang, Kunlun; Luo, Yunbo; Xu, Wentao

doi:10.1007/s00604-019-3996-2

Cited by 24 publications

(10 citation statements)

References 32 publications

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“…The G-rich sequences associate with hemin to form massive peroxidase-mimicking DNAzymes [31], which can catalyze the oxidation of the colorless ABTS 2− to green-colored ABTS 2+ in the presence of H 2 O 2 . The concentration of hemin, the G-4/hemin incubation time, and the incubation temperature wielded great influence on the formation of the G-4/hemin complex, thereby affecting the catalytic oxidation of ABTS.…”

Section: Optimization Of G-4/hemin Colorimetric Conditionsmentioning

confidence: 99%

A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach

Chen

Zhang

Liu

et al. 2021

Foods

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The detection of aflatoxin B1 (AFB1) has recently garnered much attention on the issue of food safety. In this study, a novel and sensitive aptasensor towards AFB1 is proposed using an Exonuclease III (Exo III)-integrated signal amplification strategy. This reported sensing strategy is regulated by aptamer-functionalized nanobeads that can target AFB1; furthermore, complementary DNA (cDNA) strands can lock the immobilized aptamer strands, preventing the signal amplification function of Exo III in the absence of AFB1. The presence of AFB1 triggers the displacement of cDNA, which will then activate the Exo III-integrated signal amplification procedure, resulting in the generation of a guanine (G)-rich sequence to form a G-4/hemin DNAzyme, which can catalyze the substrate of ABTS to produce a green color. Using this method, a practical detection limit of 0.0032 ng/mL and a dynamic range of detection from 0.0032 to 50 ng/mL were obtained. Additionally, the practical application of the established sensing method for AFB1 in complex matrices was demonstrated through recovery experiments. The recovery rate and relative standard deviations (RSD) in three kinds of cereal samples ranged from 93.83% to 111.58%, and 0.82% to 7.20%, respectively, which were comparable with or better than previously reported methods.

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Section: Optimization Of G-4/hemin Colorimetric Conditionsmentioning

confidence: 99%

A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach

Chen

Zhang

Liu

et al. 2021

Foods

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“…A detection of 3.5 nM Zn 2 + was achieved and lake water was used as a real sample matrix, although no comparison was made with potential interference from Pb 2 + . [78]…”

Section: Biosensors For Zn 2 +mentioning

confidence: 99%

“…Recently, the same group also coupled the cleavage reaction to the production of a peroxidase‐mimicking DNAzyme with a colorimetric output. A detection of 3.5 nM Zn 2+ was achieved and lake water was used as a real sample matrix, although no comparison was made with potential interference from Pb 2+ [78] …”

Section: Introductionmentioning

confidence: 99%

Zn²⁺‐Dependent DNAzymes: From Solution Chemistry to Analytical, Materials and Therapeutic Applications

2020

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Since 1994, deoxyribozymes or DNAzymes have been in vitro selected to catalyze various types of reactions. Metal ions play a critical role in DNAzyme catalysis, and Zn2+ is a very important one among them. Zn2+ has good biocompatibility and can be used for intracellular applications. Chemically, Zn2+ is a Lewis acid and it can bind to both the phosphate backbone and the nucleobases of DNA. Zn2+ undergoes hydrolysis even at neutral pH, and the partially hydrolyzed polynuclear complexes can affect the interactions with DNA. These features have made Zn2+ a unique cofactor for DNAzyme reactions. This review summarizes Zn2+‐dependent DNAzymes with an emphasis on RNA‐/DNA‐cleaving reactions. A key feature is the sharp Zn2+ concentration and pH‐dependent activity for many of the DNAzymes. The applications of these DNAzymes as biosensors for Zn2+, as therapeutic agents to cleave intracellular RNA, and as chemical biology tools to manipulate DNA are discussed. Future studies can focus on the selection of new DNAzymes with improved performance and detailed biochemical characterizations to understand the role of Zn2+, which can facilitate practical applications of Zn2+‐dependent DNAzymes.

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“…DNAzymes are DNA-based catalysts; all known DNAzymes have been selected through in vitro selection [ 8 , 21 , 22 ]. There are many DNAzymes that require divalent metal ions such as Pb 2+ [ 23 , 24 , 25 ], Zn 2+ [ 26 , 27 ], Cu 2+ [ 28 , 29 ], and UO 2 2+ [ 30 , 31 ], for activity. DNAzymes have several advantages compared to enzymes commonly used within biosensing: they can easily be grafted onto surfaces, are more stable at ambient conditions, and can amplify detection due to their catalytic nature [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

DNAzyme Sensor for the Detection of Ca2+ Using Resistive Pulse Sensing

Heaton

Platt

2020

Sensors

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DNAzymes are DNA oligonucleotides that can undergo a specific chemical reaction in the presence of a cofactor. Ribonucleases are a specific form of DNAzymes where a tertiary structure undergoes cleavage at a single ribonuclease site. The cleavage is highly specificity to co-factors, which makes them excellent sensor recognition elements. Monitoring the change in structure upon cleavage has given rise to many sensing strategies; here we present a simple and rapid method of following the reaction using resistive pulse sensors, RPS. To demonstrate this methodology, we present a sensor for Ca2+ ions in solution. A nanoparticle was functionalised with a Ca2+ DNAzyme, and it was possible to follow the cleavage and rearrangement of the DNA as the particles translocate the RPS. The binding of Ca2+ caused a conformation change in the DNAzyme, which was monitored as a change in translocation speed. A 30 min assay produced a linear response for Ca2+ between 1–9 μm, and extending the incubation time to 60 min allowed for a concentration as low as 0.3 μm. We demonstrate that the signal is specific to Ca2+ in the presence of other metal ions, and we can quantify Ca2+ in tap and pond water samples.

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A colorimetric zinc(II) assay based on the use of hairpin DNAzyme recycling and a hemin/G-quadruplex lighted DNA nanoladder

Cited by 24 publications

References 32 publications

A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach

A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach

Zn²⁺‐Dependent DNAzymes: From Solution Chemistry to Analytical, Materials and Therapeutic Applications

DNAzyme Sensor for the Detection of Ca2+ Using Resistive Pulse Sensing

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A colorimetric zinc(II) assay based on the use of hairpin DNAzyme recycling and a hemin/G-quadruplex lighted DNA nanoladder

Cited by 24 publications

References 32 publications

A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach

A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach

Zn2+‐Dependent DNAzymes: From Solution Chemistry to Analytical, Materials and Therapeutic Applications

DNAzyme Sensor for the Detection of Ca2+ Using Resistive Pulse Sensing

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Product

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Zn²⁺‐Dependent DNAzymes: From Solution Chemistry to Analytical, Materials and Therapeutic Applications