A colorimetric, ratiometric and water-soluble fluorescent probe for simultaneously sensing glutathione and cysteine/homocysteine

Dai, Xi; Wang, Zhao-Yang; Du, Zhi-Fang; Cui, Jie; Jiang, Miao; Zhao, Bao‐Xiang

doi:10.1016/j.aca.2015.10.023

Cited by 93 publications

(27 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering the protonationdeprotonation of the NRPCNSs and the high pH can lead to the formation of the insoluble Ag 2 O from Ag(OH) which formed under strong alkaline conditions, thus leading to incomplete FL quenching and restoration, the mild pH at 7.4 of PBS solution was recommended for use in this work. , which is lower or comparable to those reported from other fluorescence-based methods [28,[44][45][46][47][48]. e high sensitivity could be attributed to the formation of the robust Ag-S bond.…”

Section: Optimization Of the Biosensorcontrasting

confidence: 48%

“Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging

Meng

Qiao

Zhao

et al. 2019

Journal of Chemistry

View full text Add to dashboard Cite

In this work, a sensitive and selective turn-on fluorimetric method has been developed for the determination of biothiols based on blocking Ag+-induced fluorescence quenching of nitrogen-rich polymer carbon nanostrips (NRPCNSs). Ag+ ion can induce the fluorescence quenching of NRPCNSs due to the formation of nonfluorescent coordination complexes via robust Ag-N interaction. Once addition of biothiols, such as cysteine (Cys) and glutathione (GSH), Ag+ ions prefer to interact with biothiols rather than NRPCNSs, which could be attribute to the formation of Ag-S bond, thus leading to effective fluorescent recovery of NRPCNSs. Under the optimized conditions, excellent linear relationships existed between the recovery degree of the NRPCNSs and the concentrations of Cys and GSH in the range of 0.05 μM to 10 μM and 0.2 μM to 30 μM, respectively. And, the limits of detection (LODs) for Cys and GSH are 16.5 nM and 65.1 nM, respectively. The detection system also shows high selectivity against other non-thiol amino acids. Moreover, the potential in practical applications of this proposed method has been demonstrated by detecting biothiols in human serum and fluorescence imaging of biothiols in living cells.

show abstract

Section: Optimization Of the Biosensorcontrasting

confidence: 48%

“Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging

Meng

Qiao

Zhao

et al. 2019

Journal of Chemistry

View full text Add to dashboard Cite

show abstract

“…Using this strategy, probes have been reported with characteristic responses to different transition metals, 63,64 ROS 65 or biothiols. [66][67][68][69][70][71][72] Theoretically, if the probe concentration, kinetics and thermodynamics of the reactions with the targets do not lead to saturation of the probe with one analyte alone, these systems will be capable of reporting on the presence of one or more analytes.…”

Section: Probes That Rely On Competition Between Analytesmentioning

confidence: 99%

Fluorescent probes for the simultaneous detection of multiple analytes in biology

2018

View full text Add to dashboard Cite

Many of the key questions facing cellular biology concern the location and concentration of chemical species, from signalling molecules to metabolites to exogenous toxins. Fluorescent sensors (probes) have revolutionised the understanding of biological systems through their exquisite sensitivity to specific analytes. Probe design has focussed on selective sensors for individual analytes, but many of the most pertinent biological questions are related to the interaction of more than one chemical species. While it is possible to simultaneously use multiple sensors for such applications, data interpretation will be confounded by the fact that sensors will have different uptake, localisation and metabolism profiles. An alternative solution is to instead use a single probe that responds to two analytes, termed a dual-responsive probe. Recent progress in this field has yielded exciting probes, some of which have demonstrated biological application. Here we review work that has been carried out to date, and suggest future research directions that will harness the considerable potential of dual-responsive fluorescent probes.

show abstract

“…The sensing mechanisms involved are different due to the different probe structures, including conjugate addition‐ cyclization reaction, sulfonamide and sulfonate bond cleavage, cyclization with aldehydes, metal complex related oxidation‐reduction, Michael addition reaction, and thiol‐ halogen nucleophilic substitution . However, most of the probes for detecting Hcy are limited in practice due to poor sensitivity, insufficient selectivity, insufficient anti‐interference ability, complex synthesis, and complicated structure. The explicit comparison between probe APTC and these representative probes was generalized in Table S1.…”

Section: Background and Originality Contentmentioning

confidence: 99%

An Intramolecular Charge Transfer and Aggregation Induced Emission Enhancement Fluorescent Probe Based on 2‐Phenyl‐1,2,3‐triazole for Highly Selective and Sensitive Detection of Homocysteine and Its Application in Living Cells

et al. 2019

View full text Add to dashboard Cite

Summary of main observation and conclusion In this work, a new simple and readily synthesized turn‐on probe 2‐(4‐anthracene‐9‐yl‐phenyl)‐ 2H‐[1,2,3]triazole‐4‐carbaldehyde (APTC) was legitimately designed towards homocysteine (Hcy). Moreover, APTC has excellent optical properties such as intramolecular charge transfer (ICT) and aggregation induced emission enhancement (AIEE) characteristics, indicating its extensive application potentiality. What's more, APTC displayed rapid, high selectivity and specificity towards homocysteine over cysteine/glutathione. The detection limit of APTC for Hcy was as low as 2.198×10–8 mol·L–1, and the response time was only 5 min. APTC has been successfully applied to detect Hcy in silica gel plates and living cells, which indicates that APTC has good stability and biocompatibility as a selective probe for Hcy. Finally, the mechanism was studied using 1H NMR titration experiments and mass spectrometry.

show abstract

A colorimetric, ratiometric and water-soluble fluorescent probe for simultaneously sensing glutathione and cysteine/homocysteine

Cited by 93 publications

References 48 publications

“Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging

“Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging

Fluorescent probes for the simultaneous detection of multiple analytes in biology

An Intramolecular Charge Transfer and Aggregation Induced Emission Enhancement Fluorescent Probe Based on 2‐Phenyl‐1,2,3‐triazole for Highly Selective and Sensitive Detection of Homocysteine and Its Application in Living Cells

Contact Info

Product

Resources

About