A colorimetric method for the determination of thiosulfate

Characterization and Interaction Studies of Two Isoforms of the Dual Localized 3-Mercaptopyruvate Sulfurtransferase TUM1 from Humans

“…3-Mercaptopyruvate:Cyanide Sulfurtransferase Activity-3-Mercaptopyruvate:cyanide sulfurtransferase activities of TUM1 were quantified by the method described by Sörbo (33). The reaction mixtures contained 3-MP at varying concentrations of 0.5-5 mM, and DTT at 2-20 mM in a final volume of 500 l of 100 mM CAPS, pH 10.5.…”

“…Steady-state Kinetics of TUM1-The kinetic constants of the TUM1 variants were determined as bisubstrate kinetics by quantification of produced sulfide or thiocyanate (33,42). As shown in Table 2 interaction of the two human TUM1-Iso1 and TUM1-Iso2 isoforms with the L-cysteine desulfurase NFS1 and the rhodaneselike protein MOCS3 from humans.…”

Dual Role of the Molybdenum Cofactor Biosynthesis Protein MOCS3 in tRNA Thiolation and Molybdenum Cofactor Biosynthesis in Humans

“…Thiosulfate Sulfurtransferase Activity of MOCS3-The sulfurtransferase activity of MOCS3 was measured according to the procedure described by Sörbo (29) in 100 mM Hepes (pH 8.0). For determination of the K m and k cat values, each reaction contained 130 nM MOCS3, 5-500 mM sodium thiosulfate, and 0.1-50 mM potassium cyanide in a total volume of 500 l. Thiocyanate was quantified as iron complex by its absorption at 460 nm (⑀ ϭ 4200 M Ϫ1 cm Ϫ1 ).…”

Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes

“…Micromolar concentrations of thiosulfate and tetrathionate were assayed by the cyanolytic procedure of Kelly et al (1969). Elemental sulfur was analyzed by cyanolysis after 12 h extraction of the cell pellet with acetone (Sörbo 1957). Cell protein was measured colorimetrically by the Lowry method using bovine serum albumin as standard after removal of interfering sulfur compounds.…”