A colorimetric assay for the determination of 5′-nucleotidase activity

Perez, Sonia A.; Courtis, Nelly; Kokkinopoulos, D.; Papamichail, Michael; Tsiapalis, C.M.; Trangas, Theoni

doi:10.1016/0022-1759(87)90218-3

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…G-6-Pase, acid phosphatase and 5'-nucleotidase were determined by the assessment of the release of inorganic phosphorus colorimetrically at 660 nm [20][21][22].…”

Section: Specific Biomarker Enzymes For Cell Organelles and Total Promentioning

confidence: 99%

In vitro produced glycoalkaloids from Solanum nigrum L. and evaluation of their potential role as antibilharziasis

Al-Ashaal¹,

Abduallah²,

Farghaly³

et al. 2017

APJTD

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“…G-6-Pase, acid phosphatase and 5'-nucleotidase were determined by the assessment of the release of inorganic phosphorus colorimetrically at 660 nm [20][21][22].…”

Section: Specific Biomarker Enzymes For Cell Organelles and Total Promentioning

confidence: 99%

In vitro produced glycoalkaloids from Solanum nigrum L. and evaluation of their potential role as antibilharziasis

Al-Ashaal¹,

Abduallah²,

Farghaly³

et al. 2017

APJTD

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Potentiometric determination of serum 5′‐nucleotidase

Rechnitz

Chen³

1988

Clinical Laboratory Analysis

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An ammonia gas sensor was used to de-corrected for by using the inhibitor concantermine 5'-nucleotidase in serum through avalin A. The present method is fast and the ammonia produced from the coupled simple, and offers considerable improvereactions of 5'-nucleotidase and adeno-ments over existing methods. Results obsine deaminase. Nonspecific hydrolysis of tained using the present method compare the substrate 5'-adenosine rnonophos-well with the spectrophotornetric method phate by serum alkaline phosphatase was and are not affected by sample turbidity.

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Patterns of adenosine deaminase, ecto-5?-nucleotidase, poly(A)polymerase and surface light chain expression in chronic lymphocytic leukemias

Trangas¹,

Courtis²,

Gounaris³

et al. 1989

Blut

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The levels of activity of three enzymes have been measured in the circulating malignant lymphocytes of 47 patients with B chronic lymphocytic leukemia (CLL). These were the purine degradative enzymes, adenosine deaminase (ADA) and ecto-5'-nucleotidase (5'NT) and the enzyme responsible for the polyadenylation of mRNA, poly(A) polymerase. The patterns of activity of the above enzymes and the expression of surface immunoglobulin light chains were examined. A heterogeneity in the specific activity of the enzymes was observed which could not be attributed to variations of the percentage of B lymphocytes. A positive correlation was found between ADA and poly(A)polymerase activity (r = 0.383, p less than 0.01). Furthermore, the expression of immunoglobulin light chain phenotype was inversely related to 5'NT specific activity; CLL cases in which less than 20% of the cells expressed lambda chain phenotype, presented 5'NT specific activity of 16.7 +/- 3.3 (S.E.) nmol/h/10(6) cells, whereas in CLL cases with more than 20% of the cells expressing this phenotype the enzyme specific activity was 4.8 +/- 1.6 (S.E.) nmol/h/10(6) cells (p less than 0.02). These findings suggest that the simultaneous determination of enzymatic activities and immunological markers, might be useful in defining subsets in CLL and the subsequent clinical treatment.

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A colorimetric assay for the determination of 5′-nucleotidase activity

Cited by 4 publications

References 11 publications

In vitro produced glycoalkaloids from Solanum nigrum L. and evaluation of their potential role as antibilharziasis

In vitro produced glycoalkaloids from Solanum nigrum L. and evaluation of their potential role as antibilharziasis

Potentiometric determination of serum 5′‐nucleotidase

Patterns of adenosine deaminase, ecto-5?-nucleotidase, poly(A)polymerase and surface light chain expression in chronic lymphocytic leukemias

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