A Color Translating Ultraviolet Microscope

Land, Edwin H.; Blout, E. R.; Grey, David S.; Flower, M.; Husek, Helen; Jones, R. Clark; Matz, C. H.; Merrill, Dennis

doi:10.1126/science.109.2833.371

Cited by 53 publications

(5 citation statements)

References 9 publications

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“…Microscopy was carried out with the aid of a color-translating ultraviolet microscope, model UY 91, of the Scientific Specialties Corp., Boston Massachusetts (Land et al, 1949;Land, 1952;Shurcliff, 1952). The machine makes automati-cally controlled exposures on 35-mm spectrumanalysis film at each of three selected wave lengths, processes the film, and then projects the three images through red, green, and blue filters in superposition upon a translucent screen to produce a color-translated image.…”

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confidence: 99%

LOCALIZATION OFS-ADENOSYLMETHIONINE INCANDIDA UTILISBY ULTRAVIOLET MICROSCOPY

Svihla

Schlenk

1959

J Bacteriol

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Supplementation of the culture medium of Candida utilis (Torulopsis utilis) and of Saccharomyces cerevisiae with L-methionine results in greatly increased formation and accumulation of S-adenosylmethionine (Schlenk and DePalma, 1957a), a sulfonium compound whose structure comprises adenosine and methionine in the following way: Adenine-ribose-S-CH2 CH2 CH(NH2) C OOH &H3 Under suitable conditions, a concentration of 40 to 60 Amoles per gram of yeast cells on a dry weight basis may be obtained. Because the molecular weight of the sulfonium ion is 399, this corresponds to a concentration of 1.6 to 2.4 per cent. S-Adenosylmethionine has the typical absorption spectrum of an adenosine compound with Em 15,400 at 260 m,u. Its concentration remains essentially constant after growth and metabolism have ceased and the quantity of nucleic acids has declined. It appeared possible, therefore, to determine the location of S-adenosylmethionine within the cells by ultraviolet microscopy. As a preliminary to the optical studies and for correlation, the earlier chemical studies had to be amplified. The present report is a detailed account of our observations which have been summarized earlier (Svihla and Schlenk, 1958). MATERIALS AND METHODS Candida utilis (Torulopsis utilis) strain ATCC 9950 was used in the present experiments. The composition of the growth medium, culture conditions, and harvesting procedure have been described earlier (Schlenk and DePalma, 1957b). L-Methionine in a concentration of 4 to 6 ,umoles per ml, or a combination of S-methyl-L-methionine and L-homocysteine (4 ,umoles of each) was used as organic sulfur supplement. For

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confidence: 99%

LOCALIZATION OFS-ADENOSYLMETHIONINE INCANDIDA UTILISBY ULTRAVIOLET MICROSCOPY

Svihla

Schlenk

1959

J Bacteriol

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“…The instrument used for ultraviolet microscopy in these investigations was a modified color-translating ultraviolet microscope Model UV-91 built by Scientitle Specialties Corporation of Boston (23,24,29). Its important characteristic is rapid access to projected images of photographic negatives obtained automatically at three selected wavelengths.…”

Section: Methodsmentioning

confidence: 99%

LOCALIZATION OF CYTOPLASMIC NUCLEIC ACID DURING GROWTH AND ENCYSTMENT OF ENTAMOEBA INVADENS

Barker

Svihla

1964

The Journal of Cell Biology

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With a modified color-translating ultraviolet microscope, the distribution of material showing an absorption maximum at 265 m~t was studied in samples from whole cultures of Entamoeba invadens at intervals during growth and from cysts allowed to mature under controlled conditions. Absorption by the cytoplasm in general gradually increased as trophozoites approached the period of maximum encystment. In late trophozoites and precystic forms, the absorbing material was concentrated into small bodies which coalesced to form large crystalloids of very high specific absorption. Maximum crystallization occurred in early cysts, where cytochcmical tests have shown the large crystalloids to be ribonucleoprotein. Electron micrographs show that the crystalloids are composed of particles 200 to 300 A in diameter. During cyst maturation the amount of absorbing material per cyst is not visibly reduced, but the large bodies fragment into smaller units until finally there is only a very high diffuse absorption over the entire cyst. From these and other results the hypothesis is advanced that the large crystalloids ("chromatoid bodies") are a manifestation of a special parasite-host adaptive mechanism; ribonucleoprotein is synthesized under favorable conditions, crystallized in the resistant cyst stage, and dispersed in the newly excysted amebae thereby enabling them to establish themselves in a new host by a period of quick growth.

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“…This subject differs from fluorescence microscopy in that ultraviolet light is used for differentiation of specimen detail rather than for greater resolving power. The most interesting development in this field is the possibility of a color translating ultraviolet microscope (52,220). The use of ultraviolet phase microscopy is discussed by Bennett et al ( 41), and both Foster and Thiel (117) and Johnson (186, 187) discuss achromatic ultraviolet objectives.…”

Section: Ultraviolet Microscopymentioning

confidence: 99%

Chemical Microscopy

McCrone¹

1954

Anal. Chem.

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A Color Translating Ultraviolet Microscope

Cited by 53 publications

References 9 publications

LOCALIZATION OFS-ADENOSYLMETHIONINE INCANDIDA UTILISBY ULTRAVIOLET MICROSCOPY

LOCALIZATION OFS-ADENOSYLMETHIONINE INCANDIDA UTILISBY ULTRAVIOLET MICROSCOPY

LOCALIZATION OF CYTOPLASMIC NUCLEIC ACID DURING GROWTH AND ENCYSTMENT OF ENTAMOEBA INVADENS

Chemical Microscopy

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