A Color Reaction of Panaxadiol With Vanillin and Sulfuric Acid

Hiai, Susumu; Oura, Hikokíchi; Hamanaka, Hiroyoshi; Odaka, Yuka

doi:10.1055/s-0028-1097841

Cited by 49 publications

(28 citation statements)

References 2 publications

(3 reference statements)

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“…The extract was stored in the desiccator at 4°C until use. Contents of total saponins [17], total flavonoids [18], total iridoids [19], and total polysaccharides [20] in BDT extract were measured by modified methods, using ginsenoside Re, quercetin, 8-acetylharpagide, and dextran as standards, respectively. The results indicated that BDT contained saponins, flavonoids, total iridoids, and total polysaccharides at concentrations of 0.91%, 0.52%, 0.66%, and 4.36% (w/w), respectively.…”

Section: Methodsmentioning

confidence: 99%

Involvement of the Cerebral Monoamine Neurotransmitters System in Antidepressant-Like Effects of a Chinese Herbal Decoction, Baihe Dihuang Tang, in Mice Model

Chen¹,

Gao²,

Xin-rong³

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Baihe Dihuang Tang (BDT) is a renowned Chinese herbal formula which is commonly used for treating patients with mental instability, absentmindedness, insomnia, deficient dysphoria, and other psychological diseases. These major symptoms closely associated with the depressive disorders. BDT was widely popular use for treating emotion-thought disorders for many years in China. In the present study, the antidepressant-like effect of BDT in mice was investigated by using the forced swim test (FST) and the tail suspension test (TST). The underlying mechanism was explored by determining the effect of BDT on the level of cerebral monoamine neurotransmitters. BDT (9 and 18 g/kg, p.o. for 14 days) administration significantly reduced the immobility time in both the FST and the TST without changing locomotion in the open field-test (OFT). Moreover, BDT treatment at the dose of 18 g/kg inhibited reserpine-induced ptosis. Meanwhile, BDT enhanced 5-HT and NA levels in mouse cerebrum as well as decreased the ratio of 5-HT compared to its metabolite, 5-HIAA, (turnover, 5-HIAA/5-HT) after TST. The results demonstrated that the antidepressant-like effect of BDT is mediated, at least partially, via the central monoaminergic neurotransmitter system.

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Section: Methodsmentioning

confidence: 99%

Involvement of the Cerebral Monoamine Neurotransmitters System in Antidepressant-Like Effects of a Chinese Herbal Decoction, Baihe Dihuang Tang, in Mice Model

Chen¹,

Gao²,

Xin-rong³

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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“…Their phytochemical groups were qualitatively and quantitatively determined by methods previously described (Dominguez, 1973;Farnsworth, 1966). Triterpenes were assayed by colorimetric analysis using ursolic acid as standard as previously described (Hiai et al, 1975). Phenolic acids were determined according to the method of Nichiforesco and Coucou (1965).…”

Section: Methodsmentioning

confidence: 99%

Antihepatotoxic activity ofRosmarinus tomentosus in a model of acute hepatic damage induced by thioacetamide

et al. 2000

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R. tomentosus is a vegetal species closely related to the culinary rosemary (R. officinalis), a plant reported to contain antihepatotoxic agents. A dried ethanol extract of the aerial parts of Rosmarinus tomentosus (Lamiaceae) and its major fraction separated by column chromatography (fraction F19) were evaluated for antihepatotoxic activity in rats with acute liver damage induced by a single oral dose of thioacetamide. Silymarin was used as a reference antihepatotoxic substance.Pre-treatment with R. tomentosus ethanol extract, fraction F19 or silymarin significantly reduced the impact of thioacetamide toxicity on plasma protein and urea levels as well as on plasma aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase and gamma-glutamyl transpeptidase activities compared with thioacetamide-treated animals (group T). Pre-treatment with R. tomentosus ethanol extract significantly reduced the impact of thioacetamide damage on alkaline phosphatase and gamma-glutamyl transpeptidase activities compared with group T. Silymarin administration significantly reduced alkaline phosphatase and gamma-glutamyl transpeptidase activities compared with group T. Fraction F19 administration reduced only alkaline phosphatase activity compared with group T.According to these data, R. tomentosus extract shows promising antihepatotoxic activity, suggesting the need to isolate the chemical principles responsible for this activity and to study this activity in a model of thioacetamide-induced cirrhosis.

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“…The assay, based on the fact that the hydrolysis of the saponins yields panaxadiol, panaxatriol, and oleanolic acid genins, is incapable of quantitatively or qualitatively evaluating the individual panaxosides. A colorimetric reaction with vanillin-sulfuric acid was proposed for the spectrophotometric determination of total saponin content (10). Methods developed using rod-TLC (11) and countercurrent chromatography (12) for the quantitative and qualitative evaluation of individual panaxosides are reliable but not readily available for the large-scale screening of commercial products.…”

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confidence: 99%