A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome

Clarke, Louise; Carbon, John

doi:10.1016/0092-8674(76)90055-6

Cited by 1,288 publications

(545 citation statements)

References 13 publications

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“…This plasmid had been reported to carry the arglF gene [41. The M r measured in [4] using the electron microscope was in good accord with that estimated by us. Accordingly, the plasmid must carry the ppc ÷ and argH* genes, both of which are known to be located at the same map position of 89 min on E. coli chromosome [15].…”

Section: Discussionsupporting

confidence: 89%

“…We report that JA20O/pLC20-IO is a fit strain for our purpose among the gene bank of [4]. The enzyme of this strain showed a higher level (--lo-fold) than that of the wild strain.…”

Section: Introductionmentioning

confidence: 90%

“…The authors are grateful to Dr K. Ueda and Dr O. Hayaishi for the use of their copy of the gene bank prepared by Clarke and Carbon [4]. They are greatly indebted also to Dr S. Nakanishi, Dr S. Numa, Dr S. Hiraga, Dr T. Ikemura and Mrs C. Wada for valuable advice and discussion.…”

Section: Acknowledgementsmentioning

confidence: 96%

“…As a starting organism we used the series of 2100 cloned E. coli strains prepared in [4]. Each clone contains a ColE1 plasmid into which a fragment ofE.…”

Section: Identification Of Plasmid Carrying the Ppc ÷ Genementioning

confidence: 99%

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Increased synthesis of phosphoenolpyruvate carboxylase in a strain of Escherichia coli bearing a ColE1‐ppc⁺ hybrid plasmid

1981

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Section: Discussionsupporting

confidence: 89%

“…We report that JA20O/pLC20-IO is a fit strain for our purpose among the gene bank of [4]. The enzyme of this strain showed a higher level (--lo-fold) than that of the wild strain.…”

Section: Introductionmentioning

confidence: 90%

Section: Acknowledgementsmentioning

confidence: 96%

“…As a starting organism we used the series of 2100 cloned E. coli strains prepared in [4]. Each clone contains a ColE1 plasmid into which a fragment ofE.…”

Section: Identification Of Plasmid Carrying the Ppc ÷ Genementioning

confidence: 99%

See 2 more Smart Citations

Increased synthesis of phosphoenolpyruvate carboxylase in a strain of Escherichia coli bearing a ColE1‐ppc⁺ hybrid plasmid

1981

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“…Considering the above percentage, a 131-kb average insert size and assuming that the genome size of durum wheat is 13,000 Mb, we estimated the coverage of the library to be 5.1-fold genome equivalents. This coverage provides a 99.4% probability of recovering any gene present in each of the two genomes of durum wheat (Clarke and Carbon 1976).…”

Section: Characterization Of the Durum Wheat Bac Librarymentioning

confidence: 99%

Construction and characterization of a half million clone BAC library of durum wheat (Triticum turgidum ssp. durum)

et al. 2003

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Durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, genomes AB) is an economically important cereal used as the raw material to make pasta and semolina. In this paper we present the construction and characterization of a bacterial artificial chromosome (BAC) library of tetraploid durum wheat cv. Langdon. This variety was selected because of the availability of substitution lines that facilitate the assignment of BACs to the A and B genome. The selected Langdon line has a 30-cM segment of chromosome 6BS from T. turgidum ssp. dicoccoides carrying a gene for high grain protein content, the target of a positional cloning effort in our laboratory. A total of 516,096 clones were organized in 1,344 384-well plates and blotted on 28 high-density filters. Ninety-eight percent of these clones had wheat DNA inserts (0.3% chloroplast DNA, 1.4% empty clones and 0.3% empty wells). The average insert size of 500 randomly selected BAC clones was 131 kb, resulting in a coverage of 5.1-fold genome equivalents for each of the two genomes, and a 99.4% probability of recovering any gene from each of the two genomes of durum wheat. Six known copy-number probes were used to validate this theoretical coverage and gave an estimated coverage of 5.8-fold genome equivalents. Screening of the library with 11 probes related to grain storage proteins and starch biosynthesis showed that the library contains several clones for each of these genes, confirming the value of the library in characterizing the organization of these important gene families. In addition, characterization of fingerprints from colinear BACs from the A and B genomes showed a large differentiation between the A and B genomes. This library will be a useful tool for evolutionary studies in one of the best characterized polyploid systems and a source of valuable genes for wheat. Clones and high-density filters can be requested at

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Construction and Application of Genomic DNA Libraries

Kim¹,

Yang²,

Kudrna³

et al. 2004

Handbook of Plant Biotechnology

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Crop failures, pathogenic outbreaks and famine are serious problems facing society. Understanding an organism's genome will help provide the genetic tools needed to solve these complex problems in a shorter time and with less effort. To efficiently study an organism's genome, it can be partitioned into a permanent and stable collection of DNA fragments, called a library. Such libraries provide convenient access to a genome for both laboratory and breeding applications. Genomic libraries can be used as substrates to physically map and sequence entire genomes, clone agriculturally important genes and to investigate gene expression patterns. Further, genomic libraries also provide powerful tools and resources for evaluating germplasm conservation stocks and biological diversity. The ongoing explosion of genetic data and molecular clone resources has opened new scientific possibilities to researchers venturing across all avenues of applied science. In this chapter we will present the details for small and large insert library construction and their applications.

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A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome

Cited by 1,288 publications

References 13 publications

Increased synthesis of phosphoenolpyruvate carboxylase in a strain of Escherichia coli bearing a ColE1‐ppc⁺ hybrid plasmid

Increased synthesis of phosphoenolpyruvate carboxylase in a strain of Escherichia coli bearing a ColE1‐ppc⁺ hybrid plasmid

Construction and characterization of a half million clone BAC library of durum wheat (Triticum turgidum ssp. durum)

Construction and Application of Genomic DNA Libraries

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A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome

Cited by 1,288 publications

References 13 publications

Increased synthesis of phosphoenolpyruvate carboxylase in a strain of Escherichia coli bearing a ColE1‐ppc+ hybrid plasmid

Increased synthesis of phosphoenolpyruvate carboxylase in a strain of Escherichia coli bearing a ColE1‐ppc+ hybrid plasmid

Construction and characterization of a half million clone BAC library of durum wheat (Triticum turgidum ssp. durum)

Construction and Application of Genomic DNA Libraries

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Increased synthesis of phosphoenolpyruvate carboxylase in a strain of Escherichia coli bearing a ColE1‐ppc⁺ hybrid plasmid

Increased synthesis of phosphoenolpyruvate carboxylase in a strain of Escherichia coli bearing a ColE1‐ppc⁺ hybrid plasmid