A Collagenous Cementum-Derived Attachment Protein Is a Marker for Progenitors of the Mineralized Tissue-Forming Cell Lineage of the Periodontal Ligament

Liu, Hong Wei; Yacobi, Rinat; Savion, Naphtali; Narayanan, A. Sampath; Pitaru, S.

doi:10.1359/jbmr.1997.12.10.1691

Cited by 107 publications

(104 citation statements)

References 37 publications

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“…Osteogenic, chondrogenic, and adipocytic lineages represented the mesodermal germ layer. Nodular mineralized deposits characteristic to human osteoblastic cultures derived from bone marrow stromal progenitor cells [30,41] were identified with Alizarin Red S staining in 5 weeks old cultures as described elsewhere [41] (Fig. 3A).…”

Section: Differentiation Capacity Of Homscmentioning

confidence: 91%

“…hOMSC were generated by explantation as described by us elsewhere [30] and expanded in low-glucose Dulbecco's modified Eagle's medium (LG-DMEM) and 10% fetal calf serum (FCS); (expansion medium). Human embryonic stem cells (hESCs) (HES-1 cell line [31]) with a stable normal karyotype (46XX) were maintained as described in [32] and served as positive controls in assays described below.…”

Section: Cell Culturementioning

confidence: 99%

“…To assess the number of clonogenic cells cultures at P 9 to P 11 obtained from two donors (aged 21 and 61 years) were plated by limited dilution in 96-well plates in expansion medium [30] at a density of 0.33, 1, 2, and 4 cells per well. Ninety-six wells were tested for each cell density.…”

Section: Cloning Efficiencymentioning

confidence: 99%

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The Lamina Propria of Adult Human Oral Mucosa Harbors a Novel Stem Cell Population

et al. 2010

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The highly regenerative capacity of the human adult oral mucosa suggests the existence of a robust stem cell (SC) population in its lamina propria (OMLP). The purpose of this study was to characterize the availability, growth, immunophenotype, and potency of this presumable SC population. Cells positive for the embryonic stem cell transcription factors Oct4 and Sox2 and for p75 formed distinct cord-like structure in the OMLP. Regardless of donor age, trillions of cells, termed human oral mucosa stem cells (hOMSC), 95% of which express mesenchymal stromal cell markers, were simply, and reproducibly produced from a biopsy of 3-4 3 2 3 1 mm 3 . A total of 40-60% of these cells was positive for Oct4, Sox2, and Nanog and 60-80% expressed constitutively neural and neural crest SC markers. hOMSC differentiated in culture into mesodermal (osteoblastic, chondroblastic, and adipocytic), definitive endoderm and ectodermal (neuronal) lineages. Unexpectedly, hOMSC treated with dexamethasone formed tumors consisting of two germ layer-derived tissues when transplanted in severe combined immune deficiency mice. The tumors consisted of tissues produced by neural crest cells during embryogenesis-cartilage, bone, fat, striated muscle, and neural tissue. These results show that the adult OMLP harbors a primitive SC population with a distinct primitive neural-crest like phenotype and identifies the in vivo localization of putative ancestors for this population. This is the first report on ectodermal-and mesodermal-derived mixed tumors formation by a SC population derived from a nonmalignant somatic adult human tissue. STEM CELLS 2010;28:984-995 Disclosure of potential conflicts of interest is found at the end of this article.

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Section: Differentiation Capacity Of Homscmentioning

confidence: 91%

Section: Cell Culturementioning

confidence: 99%

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The Lamina Propria of Adult Human Oral Mucosa Harbors a Novel Stem Cell Population

et al. 2010

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“…Approximately 42 % of cultured adult human periodontal ligament cells are clonogenic which could be expanded by over 222 doublings [33]; therefore, these clones could represent cultured stem cell population of the periodontal ligament. About 20 % of these putative stem cells do not differentiate into mineralized tissue in the presence of dexamethasone unless they were first induced with BMP 2 [34].…”

Section: Periodontal Ligamentmentioning

confidence: 99%

“…Evidence indicates that cementum proteins in combination with BMP 2 are possible soluble factors involved in the chemotaxis and differentiation to cementoblastic lineage (Fig. 1) [33,34,66,[79][80][81][82][83].…”

Section: Stem Cell Niche and Stem Cell Differentiationmentioning

confidence: 99%

Periodontal Stem Cells: a Historical Background and Current Perspectives

Pitaru¹,

Narayanan

Etikala

et al. 2013

Curr Oral Health Rep

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In this review we discuss the historical perspective of stem cell populations from oral tissues in light of our current understanding of stem cell biology. Stem cells and their niches have been identified in the periodontium starting from the late 1970s. Applying new criteria for the identification and characterization reveals that oral tissues comprise a multipotent primitive neural crest-like stem cell population capable of differentiating into neural crest derived cell lineages of the cranial-facial zone. This population supplies cells to a more restricted stem cell type with tissue specific epigenetic memory that differentiates into cell lineages characteristic of their tissue origin. We believe that the microenvironment plays an essential role in maintaining stem cell populations and directing their migration and differentiation, and that this factor needs to be considered for utilization of stem cell-based therapy for periodontal regeneration and regenerative dental medicine.

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The role of PHF8 and TLR4 in osteogenic differentiation of periodontal ligament cells in inflammatory environment

Liu

et al. 2020

Journal of Periodontology

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Background: Histone methylation is considered to play an important role in the occurrence and development of periodontitis. Plant homeodomain finger protein 8 (PHF8), a histone demethylase, has been shown to regulate inflammation and osteogenic differentiation of bone marrow stromal cells (BMSCs). This study aimed to detect the functions of PHF8 and TLR4 in osteogenic differentiation in an inflammatory environment induced by Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) Methods: A periodontitis mouse model was established, and the mice were treated with TAK-242. Immunohistochemical staining was used to detect the expression of PHF8 in periodontal tissue. Periodontal ligament cells (PDLCs) were treated with mineralization induction medium supplemented with Pg-LPS and/or TAK-242, and a Cell Counting Kit-8 (CCK-8) assay was used to detect the proliferation of PDLCs. Real-time PCR and western blotting were used to detect the mRNA and protein expression levels, respectively, of PHF8, toll-like receptor 4 (TLR4) and the other osteogenic markers alkaline phosphatase (ALP), osteocalcin (OCN), Special AT-rich sequence-binding protein 2 (Satb2) and Runt-related transcription factor 2 (Runx2) Results: Periodontitis reduced PHF8 expression in periodontal tissue, and TAK-242 partially reversed this downregulation. An in vitro experiment revealed that the mRNA and protein expression levels of PHF8 were significantly upregulated during the osteogenic differentiation of PDLCs. Alizarin red staining showed that the mineralized nodules of PDLCs in osteogenic induction group were more than those in control group. Real-time PCR and western blot results indicated that Pg-LPS inhibited PHF8 expression and upregulated TLR4 expression in PDLCs.TAK-242 inhibited TLR4 and partially reversed the inhibition of PHF8 expression and osteogenic differentiation induced by Pg-LPS in PDLCs Conclusion: PHF8 and TLR4 play important roles in periodontitis. Pg-LPS inhibits the expression of PHF8 via upregulation of TLR4 and might further inhibit the osteogenic differentiation of PDLCs. However, the specific mechanisms involved remain to be explored.

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A Collagenous Cementum-Derived Attachment Protein Is a Marker for Progenitors of the Mineralized Tissue-Forming Cell Lineage of the Periodontal Ligament

Cited by 107 publications

References 37 publications

The Lamina Propria of Adult Human Oral Mucosa Harbors a Novel Stem Cell Population

The Lamina Propria of Adult Human Oral Mucosa Harbors a Novel Stem Cell Population

Periodontal Stem Cells: a Historical Background and Current Perspectives

The role of PHF8 and TLR4 in osteogenic differentiation of periodontal ligament cells in inflammatory environment

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